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Association of Laccase from Bacillus cereus O2-B and Pseudomonas aeruginosa O1-P with the bio-degradation of polymers: an in vitro to in silico approach

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Abstract

Plastic accumulation has become a serious environmental threat. Mitigation of plastic is important to save the ecosystem of our planet. With current research being focused on microbial degradation of plastics, microbes with the potential to degrade polyethylene were isolated in this study. In vitro studies were performed to define the correlation between the degrading capability of the isolates and laccase, a common oxidase enzyme. Instrumental analyses were used to evaluate morphological and chemical modifications in polyethylene, which demonstrated a steady onset of the degradation process in case of both isolates, Pseudomonas aeruginosa O1-P and Bacillus cereus O2-B. To understand the efficiency of laccase in degrading other common polymers, in silico approach was employed, for which 3D structures of laccase in both the isolates were constructed via homology modeling and molecular docking was performed, revealing that the enzyme laccase can be exploited to degrade a wide range of polymers.

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Data availability

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article.

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Acknowledgements

We acknowledge the Nanotechnology Research Centre (NRC) and SRM Central Instrumentation Facility (SCIF), SRMIST for providing the research facilities.

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Authors and Affiliations

  1. Department of Genetic Engineering, College of Engineering & Technology (CET), SRM Institute of Science and Technology, Chennai, Tamil Nadu, India

    M Shafana Farveen & Rajnish Narayanan

  2. Computational Biology Laboratory, Department of Genetic Engineering, College of Engineering & Technology (CET), SRM Institute of Science and Technology, Chennai, Tamil Nadu, India

    Thirumurthy Madhavan

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  1. M Shafana Farveen
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Contributions

SF, RN and TM contributed to the conception, design of the experiments. SF contributed to data acquisition and drafted the manuscript. SF, RN, TM contributed to data analysis and have critically revised the manuscript. All authors gave final approval and agreed to be accountable for all aspects of the work.

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Correspondence to Rajnish Narayanan.

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Shafana Farveen, M., Madhavan, T. & Narayanan, R. Association of Laccase from Bacillus cereus O2-B and Pseudomonas aeruginosa O1-P with the bio-degradation of polymers: an in vitro to in silico approach. Biodegradation 34, 383–403 (2023). https://doi.org/10.1007/s10532-023-10028-3

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