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Optimizing the malathion degrading potential of a newly isolated Bacillus sp. AGM5 based on Taguchi design of experiment and elucidation of degradation pathway

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Abstract

Malathion, a pesticide used to control pests in crops, vegetables, fruits, and livestock. Its widespread and indiscriminate usage has ensued in different ecological issues, thus, it’s vital to remediate this insecticide. Malathion degrading bacterium Bacillus sp. AGM5, isolated from pesticide contaminated agricultural field was cultured in presence of different malathion concentrations under aerobic and energy restrictive conditions and was found effective at malathion degradation. Recovered malathion was extracted based on QuEChERS approach and then analyzed by UHPLC. About 39.5% of malathion biodegradation was observed at 300 µlL−1 after 96 h of incubation with the tested bacteria which increased to 58.5% and 72.5% after 240, and 360 h of incubation, respectively. To further enhance malathion biodegradation, the effects of co-substrates, pH, temperature, initial malathion concentration, agitation (rpm), and inoculum size were evaluated using Taguchi methodology. Taguchi DOE’s ability to predict the optimal response was established experimentally via optimised levels of these factors (glucose-0.1%, yeast extract-0.1%, inoculum size-2% wv−1, malathion concentration 300 µlL−1, rpm-150, pH-7, temperature 40 °C), whereby biodegradation rate was enhanced to 95.49% after 38 h. Confirmation of malathion biodegradation was performed by UHPLC, Q-TOF–MS, GC–MS analysis and a possible degradation pathway was proposed for malathion biodegradation. First order kinetic model was appropriate to describe malathion biodegradation. The Taguchi DOE proved to be viable tool for optimizing culture conditions and analysing the interactions between process parameters in order to attain the best feasible combination for maximum malathion degradation. These results could influence the development of a bioremediation strategy.

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Acknowledgements

The Department of Science and Technology, New Delhi, India, funded this study by awarding a fellowship to the author under the "Innovation in Science Pursuit for Inspired Research (INSPIRE) [IF170501] scheme (Mohd Ashraf Dar). MNIT, Jaipur is acknowledged for facility and guidance in analysis.

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  1. Department of Environmental Science, School of Earth Sciences, Central University of Rajasthan, BandarSindri, Ajmer, Rajasthan, 305817, India

    Mohd Ashraf Dar & Garima Kaushik

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Dar, M.A., Kaushik, G. Optimizing the malathion degrading potential of a newly isolated Bacillus sp. AGM5 based on Taguchi design of experiment and elucidation of degradation pathway. Biodegradation 33, 419–439 (2022). https://doi.org/10.1007/s10532-022-09986-x

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