Methane oxidation coupled to vanadate reduction in a membrane biofilm batch reactor under hypoxic condition

  • Zhen Wang
  • Ling-Dong Shi
  • Chun-Yu LaiEmail author
  • He-Ping ZhaoEmail author
Original Paper


This study shows vanadate (V(V)) reduction in a methane (CH4) based membrane biofilm batch reactor when the concentration of dissolved oxygen (O2) was extremely low. V(IV) was the dominant products formed from V(V) bio-reduction, and majority of produced V(IV) transformed into precipitates with green color. Quantitative polymerase chain reaction and Illumina sequencing analysis showed that archaea methanosarcina were significantly enriched. Metagenomic predictive analysis further showed the enrichment of genes associated with reverse methanogenesis pathway, the key CH4-activating mechanism for anaerobic methane oxidation (AnMO), as well as the enrichment of genes related to acetate synthesis, in archaea. The enrichment of aerobic methanotrophs Methylococcus and Methylomonas implied their role in CH4 activation using trace level of O2, or their participation in V(V) reduction.


Methane oxidation Vanadate reduction Membrane biofilm batch reactor Hypoxic condition 



Authors greatly thank the “Natural Science Funds for Distinguished Young Scholar of Zhejiang Province (LR17B070001)”, “National Natural Science Foundation of China (Grant Nos. 21577123, 51878596)” and the “National Key Technology R&D Program (2018YFC1802203)” for their financial support.

Supplementary material

10532_2019_9887_MOESM1_ESM.docx (389 kb)
Supplementary material 1 (DOCX 389 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resource ScienceZhejiang UniversityHangzhouChina
  2. 2.Zhejiang Province Key Laboratory for Water Pollution Control and Environmental SafetyZhejiang UniversityHangzhouChina
  3. 3.Advanced Water Management CentreThe University of QueenslandSt. LuciaAustralia

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