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Biodegradation

, Volume 30, Issue 2–3, pp 161–171 | Cite as

Degradation of 3,5,6-trichloro-2-pyridinol by a microbial consortium in dryland soil with anaerobic incubation

  • Shenghui WangEmail author
  • Chen Zhang
  • Zhiwei Lv
  • Huiming Huang
  • Xueting Cao
  • Zhifeng Song
  • Mingzhu Shao
Original Paper
  • 82 Downloads

Abstract

Biodegradation of 3,5,6-trichloro-2-pyridinol (TCP) in drylands is an important biological process of detoxification. Flooding in drylands can result in the formation of anaerobic habitats. However, little is known about the microbial metabolism of TCP in dryland soil under anaerobic conditions. Here, chlorpyrifos-contaminated dryland soil was incubated to enrich the TCP-degrading microbial consortium under anaerobic conditions. Chloridion and CO2 were released with TCP degradation, and the enrichment cultures of dryland soil could metabolize 97% of TCP (100 mg/L) within 20 h. Both reductive and hydrolysis dechlorination mechanisms were involved in TCP biodegradation under anaerobic conditions. Bacterial taxonomic analysis revealed that the aerobic TCP-degrading bacteria Ochrobactrum and dechlorination bacteria Delftia were the dominant genera. Anaerobic and facultative bacteria; i.e., Bacteroides, Bacillus, and Cupriavidus had lower relative abundances, but they were significantly enriched following treatment with TCP. These results indicate that the enrichment cultures of dryland soil dominated by aerobic bacteria could dechlorinate and degrade TCP under anaerobic conditions.

Keywords

3, 5, 6-trichloro-2-pyridinol Dryland soil Anaerobic incubation Degradation Bacterial community 

Notes

Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant Nos. 41401288 and 41501251), Science and Technology and Culture Innovation Fund (Grant Nos. 26312160910 and 26312170904) for College Students of Liaocheng University and the Shandong Provincial Natural Science Foundation of China (ZR2018BH043).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Abraham J, Silambarasan S (2016) Biodegradation of chlorpyrifos and its hydrolysis product 3, 5, 6-trichloro-2-pyridinol using a novel bacterium Ochrobactrum sp. JAS2: a proposal of its metabolic pathway. Pestic Biochem Physiol 126:13–21CrossRefGoogle Scholar
  2. Anwar S, liaquat F, Khan QW, Khalid ZM, Igbal S (2009) Biodegradation of chlorpyrifos and its hydrolysis product 3, 5, 6-trichloro-2-pyridinol by Bacillus pumilus strain C2A1. J Hazard Mater 168(1):400–405CrossRefGoogle Scholar
  3. Cáceres T, He W, Naidu R, Megharaj M (2007) Toxicity of chlorpyrifos and TCP alone and in combination to Daphnia carinata: the influence of microbial degradation in natural water. Water Res 41(19):4497–4503CrossRefGoogle Scholar
  4. Cao L, Liu H, Zhang H, Huang K, Gu T, Ni H, Hong Q, Li SP (2012) Characterization of a newly isolated highly effective 3, 5, 6-Trichloro-2-pyridinol degrading strain Cupriavidus pauculus P2. Curr Microbiol 65(3):231–236CrossRefGoogle Scholar
  5. Chen K, Jian SS, Huang LL, Ruan ZP, Li SP, Jiang JD (2015) Reductive dehalogenation of 3, 5-dibromo-4-hydroxybenzoate by an aerobic strain of Delftia sp. EOB-17. Biotechnol Lett 37(12):2395–2401CrossRefGoogle Scholar
  6. Cui N, Wang S, Khorram MS, Fang H, Yu Y (2018) Microbial degradation of fomesafen and detoxification of fomesafen-contaminated soil by the newly isolated strain Bacillus sp. FE-1 via a proposed biochemical degradation pathway. Sci Total Environ 616–617:1612–1619CrossRefGoogle Scholar
  7. Eschbach M, Möbitz H, Rompf A, Jahn D (2003) Members of the genus Arthrobacter grow anaerobically using nitrate ammonification and fermentative processes: anaerobic adaptation of aerobic bacteria abundant in soil. FEMS Microbiol Lett 223(2):227–230CrossRefGoogle Scholar
  8. Feng Y, Racke KD, Bollag JM (1997) Isolation and characterization of a chlorinated-pyridinol-degrading bacterium. Appl Environ Microbiol 63(10):4096–4098Google Scholar
  9. Feng Y, Minard RD, Bollag JM (1998) Photolytic and microbial degradation of 3, 5, 6-trichloro-2-pyridinol. Environ Toxicol Chem 17:814–819CrossRefGoogle Scholar
  10. Feng F, Ge J, Li Y, He S, Zhong L, Yu X (2017) Enhanced degradation of chlorpyrifos in rice (Oryza sativa L.) by five strains of endophytic bacteria and their plant growth promotional ability. Chemosphere 184:505–513CrossRefGoogle Scholar
  11. Gao ZM, Xu X, Ruan LW (2014) Enrichment and characterization of an anaerobic cellulolytic microbial consortium SDQ-1.1 from mangrove soil. Appl Microbiol Biotechnol 98(1):457–474CrossRefGoogle Scholar
  12. Han L, Zhao D, Li C (2015) Isolation and 2, 4-D-degrading characteristics of Cupriavidus campinensis BJ71. Braz J Microbiol 46(2):433–441CrossRefGoogle Scholar
  13. Jabeen H, Iqbal S, Anwar S (2015) Biodegradation of chlorpyrifos and 3, 5, 6-trichloro-2-pyridinol by a novel rhizobial strain Mesorhizobium sp. HN3. Water Environ J 29(1):151–160CrossRefGoogle Scholar
  14. Kaya D, Imamoglu I, Sanin FD, Sowers KR (2018) A comparative evaluation of anaerobic dechlorination of PCB-118 and Aroclor 1254 in sediment microcosms from three PCB-impacted environments. J Hazard Mater 341:328–335CrossRefGoogle Scholar
  15. Kim JR, Ahn YJ (2009) Identification and characterization of chlorpyrifos-methyl and 3, 5, 6-trichloro-2-pyridinol degrading Burkholderia sp. strain KR100. Biodegradation 20(4):487–497CrossRefGoogle Scholar
  16. Li JQ, Liu J, Shen WJ, Zhao XL, Hou Y, Cao H, Cui ZL (2010) Isolation and characterization of 3, 5, 6-trichloro-2-pyridinol-degrading Ralstonia sp. strain T6. Bioresour Technol 101(10):7479–7483CrossRefGoogle Scholar
  17. Li JQ, Huang Y, Hou Y, Li XM, Cao H, Cui ZL (2013) Novel gene clusters and metabolic pathway involved in 3,5,6-Trichloro-2-Pyridinol degradation by Ralstonia sp. Strain T6. Appl Environ Microbiol 79(23):7445–7453CrossRefGoogle Scholar
  18. Liu Z, Yang C, Qiao CL (2007) Biodegradation of p-nitrophenol and 4-chlorophenol by Stenotrophomonas sp. FEMS Microbiol Lett 277(2):150–156CrossRefGoogle Scholar
  19. Lü Z, Lu YH (2012) Methanocella conradii sp. nov., a thermophilic, obligate hydrogenotrophic methanogen, isolated from Chinese rice field soil. PLoS ONE 7:35279CrossRefGoogle Scholar
  20. Lu P, Li QF, Liu HM, Feng ZZ, Yan X, Hong Q, Li SP (2013) Biodegradation of chlorpyrifos and 3, 5, 6-trichloro-2-pyridinol by Cupriavidus sp. DT-1. Bioresour Technol 127:337–342CrossRefGoogle Scholar
  21. Manickam N, Reddy MK, Saini HS, Shanker R (2008) Isolation of hexachlorocyclohexane-degrading Sphingomonas sp. by dehalogenase assay and characterization of genes involved in gamma-HCH degradation. J Appl Microbiol 104(4):952–960CrossRefGoogle Scholar
  22. Olaniran AO, Igbinosa EO (2011) Chlorophenols and other related derivatives of environmental concern: properties, distribution and microbial degradation processes. Chemosphere 83(10):1297–1306CrossRefGoogle Scholar
  23. Racke KD (1993) Environmental fate of chlorpyrifos. Rev Environ Contam Toxicol 131:1–150Google Scholar
  24. Rayu S, Nielsen UN, Nazaries L, Singh BK (2017) Isolation and molecular characterization of novel chlorpyrifos and 3, 5, 6-trichloro-2-pyridinol-degrading bacteria from sugarcane farm soils. Front Microbiol 8:518CrossRefGoogle Scholar
  25. Rios-Covian D, Gueimonde M, Duncan SH, Flint HJ, de los Reyes-Gavilan CG (2015) Enhanced butyrate formation by cross-feeding between Faecalibacterium prausnitzii and Bifidobacterium adolescentis. FEMS Microbiol Lett 362(21):1–7CrossRefGoogle Scholar
  26. Shiratori H, Ohiwa H, Ikeno H, Ayames S, Kataoka N, Miya A, Beppu T, Ueda K (2008) Lutispora thermophila gen. nov., sp. nov., a thermophilic, spore-forming bacterium isolated from a thermophilic methanogenic bioreactor digesting municipal solid wastes. Int J Syst Evol Microbiol 58:964–969CrossRefGoogle Scholar
  27. Shu DT, He YL, Yue H, Wang QY (2016) Metagenomic and quantitative insights into microbial communities and functional genes of nitrogen and iron cycling in twelve wastewater treatment systems. Chem Eng J 290:21–30CrossRefGoogle Scholar
  28. Singh BK, Walker A (2006) Microbial degradation of organophosphorus compounds. FEMS Microbiol Rev 30(3):428–471CrossRefGoogle Scholar
  29. Singh BK, Walker A, Morgan JA, Wright DJ (2004) Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Appl Environ Microbiol 70:4855–4863CrossRefGoogle Scholar
  30. Tas DO, Pavlostathis SG (2005) Microbial reductive transformation of pentachloronitrobenzene under methanogenic conditions. Environ Sci Technol 39(21):8264–8272CrossRefGoogle Scholar
  31. Tiwari MK, Guha S (2014) Kinetics of biotransformation of chlorpyrifos in aqueous and soil slurry environments. Water Res 51:73–85CrossRefGoogle Scholar
  32. Wang SH, Zhang C, Yan YC (2012) Biodegradation of methyl parathion and p-nitrophenol by a newly isolated Agrobacterium sp. strain Yw12. Biodegradation 23(1):107–116CrossRefGoogle Scholar
  33. Wang SH, Zhang C, Li K, Qu J, Shi YH, Yan YC (2013) Chlorpyrifos-induced stress response in the chlorpyrifos-degrader Klebsiella sp. CPK. Int Biodeter Biodegr 82:17–23CrossRefGoogle Scholar
  34. Wilson FP, Liu X, Mattes TE, Cupples AM (2016) Nocardioides, Sediminibacterium, Aquabacterium, Variovorax, and Pseudomonas linked to carbon uptake during aerobic vinyl chloride degradation. Environ Sci Pollut Res Int 23(19):19062–19070CrossRefGoogle Scholar
  35. Wu ZG, Wang F, Ning LQ, Stedtfeld RD, Yang ZZ, Cao JG, Sheng HJ, Jiang X (2017) Biodegradation of 5-chloro-2-picolinic acid by novel identified co-metabolizing degrader Achromobacter sp. f1. Biodegradation 28(2-3):139–144CrossRefGoogle Scholar
  36. Xu GM, Li YY, Zheng W, Peng X, Li W, Yan YC (2007) Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp. Biotechnol Lett 29(10):1469–1473CrossRefGoogle Scholar
  37. Xu GM, Zheng W, Li YY, Wang SH, Zhang JS, Yang YC (2008) Biodegradation of chlorpyrifos and 3, 5, 6-trichloro-2-pyridinol by a newly isolated Paracoccus sp. strain TRP. Int Biodeter Biodegr 62(1):51–56CrossRefGoogle Scholar
  38. Xue L, Feng X, Xu Y, Li X, Zhu M, Xu J, He Y (2017) The dechlorination of pentachlorophenol under a sulfate and iron reduction co-occurring anaerobic environment. Chemosphere 182:166–173CrossRefGoogle Scholar
  39. Yoon H, Leibeling S, Zhang CY, Müller RH, Werth CJ, Zilles JL (2014) Adaptation of Delftia acidaovorans for degradation of 2, 4-dichlorophenoxyacetate in a microfluidic porous medium. Biodegradation 25(4):595–604CrossRefGoogle Scholar
  40. Zhang C, Yuan Q, Lu YH (2014) Inhibitory effects of ammonia on methanogen mcrA transcripts in anaerobic digester sludge. FEMS Microbiol Ecol 87(2):368–377CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Shenghui Wang
    • 1
    Email author
  • Chen Zhang
    • 2
  • Zhiwei Lv
    • 1
  • Huiming Huang
    • 1
  • Xueting Cao
    • 1
  • Zhifeng Song
    • 1
  • Mingzhu Shao
    • 1
  1. 1.College of Life ScienceLiaocheng UniversityLiaochengChina
  2. 2.State Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingChina

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