, Volume 30, Issue 2–3, pp 161–171 | Cite as

Degradation of 3,5,6-trichloro-2-pyridinol by a microbial consortium in dryland soil with anaerobic incubation

  • Shenghui WangEmail author
  • Chen Zhang
  • Zhiwei Lv
  • Huiming Huang
  • Xueting Cao
  • Zhifeng Song
  • Mingzhu Shao
Original Paper


Biodegradation of 3,5,6-trichloro-2-pyridinol (TCP) in drylands is an important biological process of detoxification. Flooding in drylands can result in the formation of anaerobic habitats. However, little is known about the microbial metabolism of TCP in dryland soil under anaerobic conditions. Here, chlorpyrifos-contaminated dryland soil was incubated to enrich the TCP-degrading microbial consortium under anaerobic conditions. Chloridion and CO2 were released with TCP degradation, and the enrichment cultures of dryland soil could metabolize 97% of TCP (100 mg/L) within 20 h. Both reductive and hydrolysis dechlorination mechanisms were involved in TCP biodegradation under anaerobic conditions. Bacterial taxonomic analysis revealed that the aerobic TCP-degrading bacteria Ochrobactrum and dechlorination bacteria Delftia were the dominant genera. Anaerobic and facultative bacteria; i.e., Bacteroides, Bacillus, and Cupriavidus had lower relative abundances, but they were significantly enriched following treatment with TCP. These results indicate that the enrichment cultures of dryland soil dominated by aerobic bacteria could dechlorinate and degrade TCP under anaerobic conditions.


3, 5, 6-trichloro-2-pyridinol Dryland soil Anaerobic incubation Degradation Bacterial community 



This study was supported by the National Natural Science Foundation of China (Grant Nos. 41401288 and 41501251), Science and Technology and Culture Innovation Fund (Grant Nos. 26312160910 and 26312170904) for College Students of Liaocheng University and the Shandong Provincial Natural Science Foundation of China (ZR2018BH043).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Shenghui Wang
    • 1
    Email author
  • Chen Zhang
    • 2
  • Zhiwei Lv
    • 1
  • Huiming Huang
    • 1
  • Xueting Cao
    • 1
  • Zhifeng Song
    • 1
  • Mingzhu Shao
    • 1
  1. 1.College of Life ScienceLiaocheng UniversityLiaochengChina
  2. 2.State Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingChina

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