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Economic production and biocontrol efficiency of lipopeptide biosurfactants from Bacillus mojavenis A21

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Abstract

In the wake of an increased attention on the eco-friendly biopesticidal products and the rising market requirements for organic agents, lipopeptides compounds have been described as biological control agents which improve the overall health growth and development of plants. Nevertheless, their high production cost constitue the major flaw in their wide use to control plant diseases. The present article aims to formulate an economic media for lipopeptides production by Bacillus mojavensis A21 for application as natural fungicides for plant disease treatment. We herein demonstrated the suitability of the potato waste, as low cost substrate, for lipopeptides production. Moreover, sea water  was found to be a good mineral salts sources. In the second part of this study, we investigate the inhibitory activity of A21 lipopeptides against the phtopathogenic Fusarium sp. The in vitro test showed a minimal inhibitory concentration of about 0.3 mg/ml. The microscopic examination, of the treated Fusarium revealed an excessive lysis of the mycelia ultrastructure with destructed spores. The in vivo antagonist activity was confirmed towards the infected potato tubers. A21 lipopeptides are effective in decreasing by about 78.26% and 60.68% when applied as preventive and curative treatments, respectively, as compared to the untreated tubers.

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Funding

This work has been supported by grants from the Tunisian Ministry of Higher Education, Scientific Research and Technology and the Tunisian Ministry of Agriculture.

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Authors and Affiliations

  1. Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, B.P. 1173-3038, Sfax, Tunisia

    Hanen Ben Ayed, Noomen Hmidet & Moncef Nasri

  2. Laboratoire d’Amélioration et Protection des Ressources Génétiques de l’Olivier, Institut d’Olivier Sfax, Université de Sfax, BP1087, Sfax, Tunisia

    Manel Cheffi Azabou & Mohamed Ali Triki

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  1. Hanen Ben Ayed
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  2. Manel Cheffi Azabou
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  3. Noomen Hmidet
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  5. Moncef Nasri
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Correspondence to Hanen Ben Ayed.

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Ayed, H.B., Azabou, M.C., Hmidet, N. et al. Economic production and biocontrol efficiency of lipopeptide biosurfactants from Bacillus mojavenis A21. Biodegradation 30, 273–286 (2019). https://doi.org/10.1007/s10532-018-9864-7

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