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Evaluation of 4-bromophenol biodegradation in mixed pollutants system by Arthrobacter chlorophenolicus A6 in an upflow packed bed reactor

Biodegradation volume 25, pages 705–718 (2014)Cite this article

Abstract

Bromophenol is listed as priority pollutant by U.S. EPA, however, there is no report so far on its removal in mixed pollutants system by any biological reactor operated in continuous mode. Furthermore, bromophenol along with chlorophenol and nitrophenol are usually the major constituents of paper pulp and pesticide industrial effluent. The present study investigated simultaneous biodegradation of these three pollutants with specially emphasis on substrate competition and crossed inhibition by Arthrobacter chlorophenolicus A6 in an upflow packed bed reactor (UPBR). A 23 full factorial design was employed with these pollutants at two different levels by varying their influent concentration in the range of 250–450 mg l−1. Almost complete removal of all these pollutants and 97 % effluent toxicity removal were achieved in the UPBR at a pollutant loading rate of 1707 mg l−1 day−1 or lesser. However, at higher loading rates, the reactor performance deteriorated due to transient accumulation of toxic intermediates. Statistical analysis of the results revealed a strong negative interaction of 4-CP on 4-NP biodegradation. On the other hand, interaction effect between 4-CP and 4-BP was found to be insignificant. Among these three pollutants 4-NP preferentially degraded, however, 4-CP exerted more inhibitory effect on 4-NP biodegradation. This study demonstrated the potential of A. chlorophenolicus A6 for biodegradation of 4-BP in mixed pollutants system by a flow through UPBR system.

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Acknowledgments

The authors thank the Centre for the Environment, Indian Institute of Technology Guwahati, India, for providing the necessary facility for carrying out this research work.

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Affiliations

  1. Department of Chemistry, Environmental Science and Technology Section, Institute of Technical Education and Research, Siksha‘O’Anusandhan University, Bhubaneswar, 751030, Orissa, India

    Naresh Kumar Sahoo

  2. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781 039, India

    Kannan Pakshirajan

  3. Department of Civil Engineering, Indian Institute of Technology Guwahati, Guwahati, 781 039, India

    Pranab Kumar Ghosh

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  1. Naresh Kumar Sahoo
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  2. Kannan Pakshirajan
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  3. Pranab Kumar Ghosh
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Correspondence to Naresh Kumar Sahoo.

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Sahoo, N.K., Pakshirajan, K. & Ghosh, P.K. Evaluation of 4-bromophenol biodegradation in mixed pollutants system by Arthrobacter chlorophenolicus A6 in an upflow packed bed reactor. Biodegradation 25, 705–718 (2014). https://doi.org/10.1007/s10532-014-9693-2

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  • DOI: https://doi.org/10.1007/s10532-014-9693-2

Keywords

  • 4-Bromophenols
  • Arthrobacter chlorophenolicus A6
  • Substrate interaction
  • Biofilm reactor
  • Toxicity removal