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Inhibition and recovery of continuous electric field application on the activity of anammox biomass

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Abstract

In this study, the effects of electric field on the activity of anammox biomass were investigated. In batch mode, experimental results demonstrated that the nitrogen removal rate enhanced by 25.6 % compared with the control experiment at the electric field of 2 V/cm with application time of 20 min. However, continuous application (24 h) of electric field impacted a mal-effect on anammox biomass during the intensity between 1 and 4 V/cm. After the electric field was removed, the activity of anammox biomass could recover within 2 weeks. This implied that the mal-effect of electric field on anammox biomass was reversible. The decrease of heme c contents and crude enzyme activity demonstrated to be the main reason for the depress of the anammox biomass activity. Transmission electron microscope observation also proved the morphological change of anammox biomass under electric field.

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References

  • APHA (1995) Standard methods for the examination of water and wastewater, 19th edn. American Public Health Association, New York

    Google Scholar 

  • Berry EA, Trumpower BL (1987) Simultaneous determination of hemes a, b, and c from pyridine hemochrome spectra. Anal Biochem 161:1–15

    Article  CAS  PubMed  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Duan XM, Zhou JT, Qiao S, Wei HF (2011) Application of low intensity ultrasound to enhance the activity of anammox microbial consortium for nitrogen removal. Bioresour Technol 102:4290–4293

    Article  CAS  PubMed  Google Scholar 

  • Kartal B, Maalcke WJ, de Almeida NM, Cirpus I, Gloerich J, Geerts W, Op den Camp HJM, Harhangi HR, Janssen-Megens EM, Francoijs KJ, Stunnenberg HG, Keltjens JT, Jetten MSM, Strous M (2011) Molecular mechanism of anaerobic ammonium oxidation. Nature 479:127–130

    Article  CAS  PubMed  Google Scholar 

  • Kim TS, Kim HY, Kim BH (1990) Petroleum desulfurization by desulfovibrio desulfuricans M6 using electrochemically supplied reducing equivalent. Biotechnol Lett 12:757–760

    Article  CAS  Google Scholar 

  • Liu ST, Yang FL, Meng FG, Chen HH, Gong Z (2008) Enhanced anammox consortium activity for nitrogen removal: impacts of static magnetic field. J Biotechnol 138:96–102

    Article  CAS  PubMed  Google Scholar 

  • Matsumoto N, Yoshignaga H, Ohmura N, Ando A, Saiki H (2002) Numerical stimulation for electrochemical cultivation of iron oxidizing bacteria. Biotechnol Bioeng 78:17–23

    Article  CAS  PubMed  Google Scholar 

  • Ohmura N, Matsumoto N, Sasaki K, Saiki H (2002) Electrochemical regeneration of Fe(III) to support growth on anaerobic iron respiration. Appl Environ Microbiol 68:405–407

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Park DH, Laivenieks M, Guettler MV, Jain MK, Zeikus JG (1999) Microbial utilization of electrically reduced neutral red as the sole electron donor for growth and metabolic production. Appl Environ Microbiol 65:2912–2917

    CAS  PubMed Central  PubMed  Google Scholar 

  • Park HI, Kim DK, Choi Y, Park D (2005) Nitrate reduction using an electrode as direct donor in a biofilm-electrode reactor. Proc Biochem 40:3383–3388

    Article  CAS  Google Scholar 

  • Prosnansky M, Sakakibarab Y, Kuroda M (2002) High-rate denitrification and SS rejection by biofilm-electrode reactor (BER) combined with microfiltration. Water Res 36:4801–4810

    Article  CAS  PubMed  Google Scholar 

  • Sasaki K, Sasaki D, Morita M, Hirano S, Matsumoto N, Ohmura N, Igarashi Y (2010) Bioelectrochemical system stabilize methane fermentation from garbage slurry. Bioresour Technol 101:3415–3422

    Article  CAS  PubMed  Google Scholar 

  • Sasaki K, Hirano S, Morita M, Sasaki D, Matsumoto N, Ohmura N, Igarashi Y (2011) Bioelectrochemical system accelerate microbial growth and degradation of filter paper. Appl Microbiol Biotechnol 89:449–455

    Article  CAS  PubMed  Google Scholar 

  • Schmid MC, Walsh K, Webb R, Rijpstra WIC, vad de Pas-Schoonen K, Verbruggen MJ, Hill T, Moffett B, Fuerst J, Schouten S, Damste JS, Harris J, Shaw P, Jetten MSM, Strous M (2003) Candidatus “Scalindua brodae”, sp. Nov., Candidatus “Scalindua wagneri”, sp. Nov., two new species of anaerobic ammonium oxidizing bacteria. Syst Appl Microbiol 26:529–538

    Article  CAS  PubMed  Google Scholar 

  • Shimamura M, Nishiyama T, Shigetomo H, Toyomoto T, Kawahara Y, Furukawa K, Fujii T (2007) Isolation of a multiheme protein with features of a hydrazine-oxidizing enzyme from an anaerobic ammonium-oxidizing enrichment culture. Appl Environ Microbiol 73:1065–1072

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Strous M, Fuerst JA, Kramer EHM, Logemann S, Muzyer G, van de Pas-Schoonen KT, Webb R, Kuene JG, Jetten MSM (1999) Missing lighotroph identified as new planctomycete. Nature 400:446–449

    Article  CAS  PubMed  Google Scholar 

  • Thrash JC, van Trump JI, Weber KA, Miller E, Achenbach LA, Coates JD (2007) Electrochemical stimulation of microbial perchlorate reduction. Environ Sci Technol 41:1740–1746

    Article  CAS  PubMed  Google Scholar 

  • Trash JC, Coates JD (2008) Review: direct and indirect electrical stimulation of microbial metabolism. Environ Sci Technol 42:3921–3931

    Article  Google Scholar 

  • Tsushima I, Kindaichi T, Okabe S (2007) Quantification of anaerobic ammonium-oxidizing bacteria in enrichment cultures by real-time PCR. Water Res 41:785–794

    Article  CAS  PubMed  Google Scholar 

  • Van der Graaf AA, de Bruijn P, Robertson LA, Jetten MSM, Kuenen JG (1996) Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor. Microbiology 142:2187–2196

    Article  Google Scholar 

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Acknowledgments

This work was supported by the Natural Science Foundation of China (No. 51008045, 21377014), Science and Technology Research Projects of Liaoning Provincial Department of Education (No. L2013026), Dalian Municipal Science and Technology Projects (No. 2012E11SF067) and Fundamental Research Funds for the Central Universities (No. DUT12LK20).

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Correspondence to Sen Qiao.

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Qiao, S., Yin, X., Zhou, J. et al. Inhibition and recovery of continuous electric field application on the activity of anammox biomass. Biodegradation 25, 505–513 (2014). https://doi.org/10.1007/s10532-013-9677-7

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  • DOI: https://doi.org/10.1007/s10532-013-9677-7

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