Biodegradation

, Volume 23, Issue 4, pp 575–583 | Cite as

2,4,6-trichlorophenol (TCP) photobiodegradation and its effect on community structure

  • Yongming Zhang
  • Xuejing Pu
  • Miaomiao Fang
  • Jun Zhu
  • Lujun Chen
  • Bruce E. Rittmann
Original Paper

Abstract

The mechanisms occurring in a photolytic circulating-bed biofilm reactor (PCBBR) treating 2,4,6-trichlorophenol (TCP) were investigated using batch experiments following three protocols: photodegradation alone (P), biodegradation alone (B), and intimately coupled photodegradation and biodegradation (P&B). Initially, the ceramic particles used as biofilm carriers rapidly adsorbed TCP, particularly in the B experiments. During the first 10 min, the TCP removal rate for P&B was equal to the sum of the rates for P and B, and P&B continued to have the greatest TCP removal, with the TCP concentration approaching zero only in the P&B experiments. When phenol, an easily biodegradable compound, was added along with TCP in order to promote TCP mineralization by means of secondary utilization, P&B was superior to P and B in terms of mineralization of TCP, giving 95% removal of chemical oxygen demand (COD). The microbial communities, examined by clone libraries, changed dramatically during the P&B experiments. Whereas Burkholderia xenovorans, a known degrader of chlorinated aromatics, was the dominant strain in the TCP-acclimated inoculum, it was replaced in the P&B biofilm by strains noted for biofilm formation and biodegrading non-chlorinated aromatics.

Keywords

Biodegradation Biofilm Photolysis Community structure Trichlorophenol 

References

  1. Ali M, Sreekrishnan TR (2001) Aquatic toxicity from pulp and paper mill effluents: a review. Adv Environ Res 5(2):175–196CrossRefGoogle Scholar
  2. Alinsafi A, Evenou F, Abdulkarim EM, Pons MN, Zahraa O, Benhammou A, Yaacoubi A, Nejmeddine A (2007) Treatment of textile industry wastewater by supported photocatalysis. Dyes Pigm 74(2):439–445CrossRefGoogle Scholar
  3. American Public Health Association (APHA) (2001) Standard methods for the examination of water and wastewater, 22nd edn. American water works association and water pollution control federation, WashingtonGoogle Scholar
  4. Aranda C, Godoy F, Becerra J, Barra R, Martínez M (2003) Aerobic secondary utilization of a non-growth and inhibitory substrate 2,4,6-trichlorophenol by Sphingopyxis chilensis S37 and Sphingopyxis-like strain S32. Biodegradation 14(4):265–274PubMedCrossRefGoogle Scholar
  5. Barac T, Taghavi S, Borremans B, Provoost A, Oeyen L, Colpaert JV, Vangronsveld J, van der Lelie D (2004) Engineered endophytic bacteria improve phytoremediation of water-soluble, volatile, organic pollutants. Nat Biotechnol 22(5):583–588PubMedCrossRefGoogle Scholar
  6. Bedard DL, Unterman R, Bopp LH, Brennan MJ, Haberl ML, Johnson C (1986) Rapid assay for screening and characterizing microorganisms for the ability to degrade polychlorinated biphenyls. Appl Environ Microbiol 51(4):761–768PubMedGoogle Scholar
  7. Boyd S A, Mikesell M D, Lee J (1989) Chlorophenols in soils. In: Reactions and movement of organic chemicals in soils. Soil Science Society of America and American Society of Agronomy, Madison, 209–228Google Scholar
  8. Chang BV, Chiang CW, Yuan SY (1999) Microbial dechlorination of 2,4,6-trichlorophenol in anaerobic sewage sludge. Environ Sci Health B34:491–507Google Scholar
  9. Chu W, Law CK (2003) Treatment of trichlorophenol by catalytic oxidation process. Water Res 37(10):2339–2346PubMedCrossRefGoogle Scholar
  10. Denef VJ, Park J, Tsoi TV, Rouillard JM, Zhang H, Wibbenmeyer JA, Verstraete W, Gulari E, Hashsham SA, Tiedje JM (2004) Biphenyl and benzoate metabolism in a genomic context: Outlining genome-wide metabolic networks in Burkholderia xenovorans LB400. Appl Environ Microbiol 70(8):4961–4970PubMedCrossRefGoogle Scholar
  11. Enriquez R, Beaugiraud B, Pichat P (2004) Mechanistic implications of the effect of TiO2 accessibility in TiO2-SiO2 coatings upon chlorinated organics photocatalytic removal in water. Water Sci Technol 49(4):147–152PubMedGoogle Scholar
  12. Gardin H, Lebeault JM, Pauss A (2001) Degradation of 2,4,6-trichlorophenol (2,4,6-TCP) by co-immobilization of anaerobic and aerobic microbial communities in an upflow reactor under air-limited conditions. Appl Microbiol Biotechnol 56(3–4):524–530PubMedCrossRefGoogle Scholar
  13. Godon JJ, Zumstein E, Dabert P, Habouzit FMR (1997) Molecular microbial diversity of an anaerobic digestor as determined by small-subunit r-DNA sequence analysis. Appl Environ Microbiol 63(7):2802–2813PubMedGoogle Scholar
  14. Gómez-De Jesús A, Romano-Baez FJ, Leyva-Amezcua L, Juárez-Ramírez C, Ruiz-Ordaz N, Galíndez-Mayer J (2009) Biodegradation of 2,4,6-trichlorophenol in a packed-bed biofilm reactor equipped with an internal net draft tube riser for aeration and liquid circulation. J Hazard Mater 161(2–3):1140–1149Google Scholar
  15. Häggblom MM (1992) Microbial breakdown of halogenated aromatic pesticides and related-compounds. FEMS Microbiol Rev 103(1):29–72CrossRefGoogle Scholar
  16. Huang WJ, Fang GC, Wang CC (2005) Ananometer-ZnO catalyst to enhance the ozonation of 2,4,6-trichlorophenol in water. Colloids Surf A 260(1–3):45–51CrossRefGoogle Scholar
  17. Kazuya W, Maki T, Shigeaki H (1999) An outbreak of nonflocculating catabolic populations caused the breakdown of a phenol-digesting activated-sludge process. Appl Environ Microbiol 65(7):2813–2819Google Scholar
  18. Keith L, Telliard W (1979) Priority pollutants: I. a perspective view. Environ Sci Technol 13(4):416–423CrossRefGoogle Scholar
  19. Liu ZP, Wang BJ, Liu YH, Liu SJ (2005) Novosphingobium taihuense sp nov., a novel aromatic-compound-degrading bacterium isolated from Taihu Lake, China. Int J Sys Evol Microbiol 55(3):1229–1232CrossRefGoogle Scholar
  20. Manilal VB, Haridas A, Alexander R, Surender GD (1992) Photocatalytic treatment of toxic organics in wastewater: toxicity of photodegradation products. Water Res 26(8):1035–1038CrossRefGoogle Scholar
  21. Marsolek MD, Torres CI, Hausner M, Rittmann BE (2008) Intimate coupling of photocatalysis and biodegradation in a photocatalytic circulating-bed biofilm reactor. Biotechnol Bioeng 101(1):83–92PubMedCrossRefGoogle Scholar
  22. Namkung E, Rittmann BE (1987a) Modeling bisubstrate removal by biofilms. Biotechnol Bioeng 29(2):269–278PubMedCrossRefGoogle Scholar
  23. Namkung E, Rittmann BE (1987b) Evaluation of bisubstrate secondary utilization kinetics by biofilms. Biotechnol Bioeng 29(3):335–342PubMedCrossRefGoogle Scholar
  24. Parra S, Malato S, Pulgarin C (2002) New integrated photocatalytic-biological flow system using supported TiO2 and fixed bacteria for the mineralization of isoproturon. Appl Catal 36(2):131–144CrossRefGoogle Scholar
  25. Ramamoorthy S (1997) Chlorinated organic compounds in the environment. CRC Press, Boca RatonGoogle Scholar
  26. Reddy MP, Srinivas B, Kumari VD, Subrahmanyam M, Sharma PN (2004) An integrated approach of solar photocatalytic and biological treatment of N-containing organic compounds in wastewater. Toxicol Environ Chem 86(1–4):125–138Google Scholar
  27. Rengaraj S, Li XZ (2006) Enhanced photocatalytic activity of TiO2 by doping with Ag for degradation of 2,4,6-trichlorophenol in aqueous suspension. J Mol Catal 243(1):60–67CrossRefGoogle Scholar
  28. Sakthivel S, Neppolian B, Palanichamy M, Arabindoo B, Murugesan V (2001) Photocatalytic degradation of leather dye over ZnO catalyst supported on alumina and glass surfaces. Water Sci Technol 44(5):211–218PubMedGoogle Scholar
  29. Saw JH, Mountain BW, Feng L, Omelchenko MV, Hou S, Saito JA, Stott MB, Li D, Zhao G, Wu J, Galperin MY, Koonin EV, Makarova KS, Wolf YI, Rigden DJ, Dunfield PF, Wang L, Alam M ((2008)) Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1. Genome Biol 9((11)):R161.1–R161.16Google Scholar
  30. Scott JP, Ollis DF (1995) Integration of chemical and biological oxidation processes for water treatment; review and recommendations. Environ Prog 14(2):88–103CrossRefGoogle Scholar
  31. Sohn JH, Kwon KK, Kang JH, Jung HB, Kim SJ (2004) Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Sys Evol Microbiol 54(5):1483–1487CrossRefGoogle Scholar
  32. Stafford U, Kamat PV, Gray KA (1997) Photocatalytic degradation of 4-chlorophenol: the effects of varying TiO2 concentration and light wavelength. J Catal 167(1):25–32CrossRefGoogle Scholar
  33. Suryaman D, Hasegawa K, Kagaya S (2006) Combined biological and photocatalytic treatment for the mineralization of phenol in water. Chemosphere 65(11):2502–2506PubMedCrossRefGoogle Scholar
  34. Tai C, Jiang GB (2005) Dechlorination and destruction of 2,4,6-trichlorophenol and pentachlorophenol using hydrogen peroxide as the oxidant catalyzed by molybdate ions under basic condition. Chemosphere 59(3):321–326PubMedCrossRefGoogle Scholar
  35. Tan IAW, Ahmad AL, Hameed BH (2009) Adsorption isotherms, kinetics, thermodynamics and desorption studies of 2,4,6-trichlorophenol on oil palm empty fruit bunch-based activated carbon. J Hazard Mater 164(2–3):473–482PubMedCrossRefGoogle Scholar
  36. USEPA (1991) Water quality criteria summary, ecological risk assessment branch (WH-585) and human risk assessment branch (WH-550D). Health and Ecological Criteria Division, USEPA, WashingtonGoogle Scholar
  37. Xia Q, Zhang XH (1990) Manual on water quality standards. Environmental Science Press, BeijingGoogle Scholar
  38. Zhang Y, Liu H, Shi W, Pu X, Rittmann BE (2010a) Photobiodegradation of phenol with ultraviolet irradiation of new ceramic biofilm carriers. Biodegradation 21(6):881–887PubMedCrossRefGoogle Scholar
  39. Zhang YM, Wang L, Rittmann BE (2010b) Integrated photocatalytic-biological reactor for accelerated phenol degradation. Appl Microbiol Biotechnol 86(6):1977–1985PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Yongming Zhang
    • 1
  • Xuejing Pu
    • 1
  • Miaomiao Fang
    • 1
  • Jun Zhu
    • 1
  • Lujun Chen
    • 2
  • Bruce E. Rittmann
    • 3
  1. 1.Department of Environmental Engineering, College of Life and Environmental ScienceShanghai Normal UniversityShanghaiPeople’s Republic of China
  2. 2.School of EnvironmentTsinghua UniversityBeijingPeople’s Republic of China
  3. 3.Swette Center for Environmental Biotechnology, Biodesign InstituteArizona State UniversityTempeUSA

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