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Biodegradation of Basic Violet 3 by Candida krusei isolated from textile wastewater

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Abstract

Basic Violet 3 (BV) belongs to the most important group of synthetic colorants and is used extensively in textile industries. It is considered as xenobiotic compound which is recalcitrant to biodegradation. As Candida krusei could not use BV as sole carbon source, experiments were conducted to study the effect of cosubstrates on decolorization of BV in semi synthetic medium using glucose, sucrose, lactose, maltose, yeast extract, peptone, urea and ammonium sulphate. Maximum decolorization (74%) was observed in media supplemented with sucrose. Use of sugarcane bagasse extract as sole nutrient source showed 100% decolorization of BV within 24 h under optimized condition. UV–visible, FTIR spectral analysis and HPLC analysis confirmed the biodegradation of BV. Six degradation products were isolated and identified. We propose the biodegradation pathway for BV which occurs via stepwise reduction and demethylation process to yield mono-, di-, tri-, tetra-, penta- and hexa-demethylated BV species which was degraded completely. The study of the enzymes responsible for decolorization showed the activities of lignin peroxidase, lacasse, tyrosinase, NADH-DCIP reductase, MG reductase and azoreductase in cells before and after decolorization. A significant increase in activities of NADH-DCIP reductase and laccase was observed in the cells after decolorization. The yeast C. krusei could show the ability to decolorize the textile dye BV using inexpensive source like sugarcane bagasse extract for decolorization.

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References

  • Au W, Pathak S, Collie CJ, Hsu TS (1978) Cytogenic toxicity of gentian violet and crystal violet on mammalian cells in vitro. Mutat Res 58:269–276

    Article  PubMed  CAS  Google Scholar 

  • Ayed L, Cheriaa J, Laadhari N, Cheref A, Bakhrouf A (2009) Biodegradation of crystal violet by an isolated Bacillus sp. Ann Microbiol 59:267–272

    Article  CAS  Google Scholar 

  • Azmi W, Sani RK, Banerjee U (1998) Biodegradation of triphenylmethane dyes. Enzyme Microb Technol 22:185–191

    Article  PubMed  CAS  Google Scholar 

  • Bumpus JA, Brock BJ (1988) Biodegradation of crystal violet by the white rot fungus Phanerocheate chrysosporium. Appl Environ Microbiol 54:1143–1150

    PubMed  CAS  Google Scholar 

  • Cha CJ, Doerge DR, Cerniglia CE (2001) Biotransformation of malachite green by the fungus Cunninghamella elegans. Appl Environ Microbiol 67:4358–4360

    Article  PubMed  CAS  Google Scholar 

  • Chen CC, Liao HJ, Cheng CY, Yen CY, Chung YC (2008a) Biodegradation of crystal violet by Pseudomonas putida. Biotechnol Lett 29:391–396

    Article  Google Scholar 

  • Chen CH, Chang CF, Ho CH, Tsai TL, Liu SM (2008b) Biodegradation of crystal violet by a Shewanella sp. NTOU1. Chemosphere 72:1712–1720

    Article  PubMed  CAS  Google Scholar 

  • Cho BP, Yang T, Blankenship LR, Moody JD, Churchwell M, Bebland FA, Culp SC (2003) Synthesis and characterization of N-demethylated metabolites of malachite green and leuco malachite green. Chem Res Toxicol 16:285–294

    Article  PubMed  CAS  Google Scholar 

  • Hatvani N, Mecs I (2001) Production of laccase and manganese peroxidase by Lentinus edodes on malt containing by product of the brewing process. Process Biochem 37:491–496

    Article  Google Scholar 

  • Henderson Al, Schmitt TC, Heinze TM, Cerniglia CE (1997) Reduction of malachite green to leucomalachite green by intestinal bacteria. Appl Environ Microbiol 63:4099–4101

    PubMed  CAS  Google Scholar 

  • Jadhav JP, Govindwar SP (2006) Biotransformation of Malachite green by MTCC 463. Yeast 23:315–323

    Article  PubMed  CAS  Google Scholar 

  • Jadhav JP, Parshetti GK, Kalme SD, Govindwar SP (2006) Decolorization of azo dye methyl red by Saccharomyces cerevisiae MTCC 463. Chemosphere 68:394–400

    Article  Google Scholar 

  • Jadhav SU, Kalme SD, Govindwar SP (2008) Biodegradation of Methyl red by Galactomyces geotrichum MTCC 1360. Int Biodeter biodegr 62:135–142

    Article  CAS  Google Scholar 

  • Jang MS, Lee YM, Kim CH, Lee JH, Kang DW, Kim SJ, Lee YC (2005) Triphenylmethane reductase from Citrobacter sp. Strain KCTC 18061P: purification, characterization, gene cloning, and overexpression of a functional protein in Escherichia coli. Appl Environ Microbiol 71:7955–7960

    Article  PubMed  CAS  Google Scholar 

  • McDonald JJ, Cerniglia CE (1984) Biotransformation of gentian violet to leucogentian violet by human, rat and chicken intestinal microflora. Drug Metab Dispos 12:330–336

    PubMed  CAS  Google Scholar 

  • Ollikka P, Alhonmaki K, Leppanen VM, Glumoff T, Raijola T, Suominen I (1993) Decolorization of azo, triphenylmethane, heterocyclic, and polymeric dyes by lignin peroxidase isoenzymes from Phanerocheate chrysosporium. Appl Environ Microbiol 59:4010–4016

    PubMed  CAS  Google Scholar 

  • Pajot HF, de Figueroa LIC, Farina JI (2007) Dye-decolorizing activity in isolated yeasts from the ecoregion of Las Yungas (Tucuman, Argentina). Enzyme Microb Technol 40:1503–1511

    Article  CAS  Google Scholar 

  • Pasti-Grigsby MB, Paszcczynski A, Goszczynski S, Crawford DL, Crawford RL (1992) Influence of aromatic substitution patterns on azo dye degradability by Streptomyces spp, and Phanerocheate chrysosporium. Appl Environ Microbiol 58:3605–3613

    PubMed  CAS  Google Scholar 

  • Pons A, Roca P, Aguiló C, Garcia FJ, Alemany M, Palou A (1981) A method for the simultaneous determination of total carbohydrate and glycerol in biological samples with the anthrone reagent. J Biochem Biophys Methods 4:227–231

    Article  PubMed  CAS  Google Scholar 

  • Raghukumar C, Chandramohan D, Michel FC, Reddy CA (1996) Degradation of lignin and decolorization of paper mill bleach plant effluent (BPE) by marine fungi. Biotechnol Lett 18:105–106

    Article  CAS  Google Scholar 

  • Ramalho PA, Cardoso MH, Paulo AC, Ramalho MT (2004) Characterization of azo reduction activity in a novel ascomycete yeast strain. Appl Environ Microbial 70:2279–2288

    Article  CAS  Google Scholar 

  • Salokhe MD, Govindwar SP (1999) Effect of carbon source on the biotransformation enzymes in Serratia marcescens. World J Microbiol Biotechnol 15:229–232

    Article  CAS  Google Scholar 

  • Sani RK, Banerjee UC (1999) Decolorization of triphenylmethane dyes and textile and dye-stuff effluent by Kurthia sp. Enzyme Microb Technol 24:433–437

    Article  CAS  Google Scholar 

  • Saratale RG, Saratale GD, Chang JS, Govindwar SP (2009) Decolorization and biodegradation of textile dye navy blue HER by Trichosporon beigelii NCIM-3326. J Hazard Mater 166:1421–1428

    Article  PubMed  CAS  Google Scholar 

  • Telke A, Kalyani D, Jadhav J, Govindwar S (2008) Kinetics and mechanism of reactive red 141 degradation by a bacterial isolate Rhizobium radiobacter MTCC 8161. Acta Chim Slov 55:320–329

    CAS  Google Scholar 

  • Vasdev K, Kuhad RC, Saxena RK (1995) Decolourization of triphenylmethane dyes by the bird’s nest fungus Cyathus bulleri. Curr Microbiol 30:269–272

    Article  Google Scholar 

  • Verma P, Madamwar D (2003) Decolorization of synthetic dyes by a newly isolated strain of Serratia marcescens. World J Microbiol Biotechnol 19:615–618

    Article  CAS  Google Scholar 

  • Wong Y, Yu J (1999) Laccase-catalysed decolourization of synthetic dyes. Water Res 33:3512–3520

    Article  CAS  Google Scholar 

  • Yatome C, Ogawa T, Matsui M (1991) Degradation of crystal violet by Bacillus subtilis. J Environ Sci Health A 26:75–87

    Article  Google Scholar 

  • Yatome C, Yamada S, Ogawa T, Matsui M (1993) Degradation of crystal violet by Nocardia coralline. Appl Microbiol Biotechnol 38:565–569

    Article  CAS  Google Scholar 

  • Young L, Yu J (1997) Ligninase-catalysed decolourization of synthetic dyes. Water Res 31:1187–1193

    Article  CAS  Google Scholar 

  • Zhang X, Flurkey W (1997) Phenol oxidases in portabella mushrooms. J Food Sci 62:97–100

    Article  CAS  Google Scholar 

  • Zhang F, Knapp JS, Tapley KN (1999) Decolorization of cotton bleaching effluent with wood rotting fungus. Water Res 33:919–928

    Article  CAS  Google Scholar 

  • Zollinger H (1987) Color chemistry-synthesis, properties and applications of organic dyes and pigments. VCH Publishers, New York, pp 92–102

    Google Scholar 

Download references

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Authors and Affiliations

  1. Environmental Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore, 632 014, Tamil Nadu, India

    Charumathi Deivasigamani & Nilanjana Das

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  1. Charumathi Deivasigamani
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  2. Nilanjana Das
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Correspondence to Nilanjana Das.

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Deivasigamani, C., Das, N. Biodegradation of Basic Violet 3 by Candida krusei isolated from textile wastewater. Biodegradation 22, 1169–1180 (2011). https://doi.org/10.1007/s10532-011-9472-2

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