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Decolourization of anaerobically digested and polyaluminium chloride treated distillery spentwash in a fungal stirred tank aerobic reactor

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Abstract

Decolourization of anaerobically digested and polyaluminium chloride treated distillery spentwash was studied in a fungal stirred tank aerobic reactor without dilution of wastewater. Aspergillus niger isolate IITB-V8 was used as the fungal inoculum. The main objectives of the study were to optimize the stirrer speed for achieving maximum decolourization and to determine the kinetic parameters. A mathematical model was developed to describe the batch culture kinetics. Volumetric oxygen transfer coefficient (k L a) was obtained using dynamic method. The maximum specific growth rate and growth yield of fungus were determined using Logistic equation and using Luedeking–Piret equation. 150 rpm was found to be optimum stirrer speed for overall decolourization of 87%. At the optimum stirrer speed, volumetric oxygen transfer coefficient (k L a) was 0.4957 min−1 and the maximum specific growth rate of fungus was 0.224 h−1. The values of yield coefficient (Y x/s) and maintenance coefficient (m s) were found to be 0.48 g cells (g substrate)−1 and 0.015 g substrate (g cells)−1 h−1.

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Abbreviations

μm :

Maximum specific growth rate (h−1)

x :

Cell concentration (g l−1)

x m :

Maximum attainable biomass concentration (g dry weight l−1)

k L a :

Volumetric oxygen transfer coefficient (min−1)

C*:

Saturated DO concentration (mg l−1)

C L :

Actual DO concentration in broth (mg l−1)

\( q_{{{\text{O}}_{2} }} \) :

Specific oxygen uptake rate (mg O2 (g cells min)−1)

m s :

Maintenance coefficient (g substrate (g cells h)−1)

Y x/s :

Yield factor for cells on carbon substrate (g cells (g substrate)−1)

References

  • APHA (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, New York

    Google Scholar 

  • Benito GG, Miranda MP, Santos DR (1997) Decolourization of wastewater from an alcoholic fermentation process with Trametes versicolor. Bioresour Technol 61:33–37

    Article  CAS  Google Scholar 

  • Chandra R, Bharagava N, Rai V (2008) Melanoidins as major colourant in sugarcane molasses based distillery effluent and its degradation. Bioresour Technol 99:4648–4660

    Article  PubMed  CAS  Google Scholar 

  • Cui YQ, Okkerse WJ, Van der Lans RGJM, Luyben KChAM (1998) Modeling and measuring of fungal growth and morphology in submerged fermentations. Biotechnol Bioeng 60:216–229

    Article  PubMed  CAS  Google Scholar 

  • Kaushik G, Thakur IS (2009) Isolation of fungi and optimization of process parameters for decolorization of distillery mill effluent. World J Microbiol Biotechnol 25:955–964

    Article  CAS  Google Scholar 

  • Kim SJ, Shoda M (1999) Batch decolourization of molasses by suspended and immobilizes fungus of Geotrichum candidum Dec 1. J Biosci Bioeng 88:586–589

    Article  PubMed  CAS  Google Scholar 

  • Lever MA (1972) New reaction for colorimetric determination of carbohydrates. Anal Biochem 47:273–279

    Article  PubMed  CAS  Google Scholar 

  • Migo VP, Matsumara M, Rosario EJD, Kataoka H (1993) Decolorization of molasses wastewater using an inorganic flocculant. J Ferment Bioeng 75(6):438–442

    Article  CAS  Google Scholar 

  • Miranda PM, Benito GG, Cristobal NS, Nieto CH (1996) Colour elimination from molasses wastewater by Aspergillus niger. Bioresour Technol 57:229–235

    Article  Google Scholar 

  • Mohana S, Acharya BK, Madamwar D (2009) Distillery spent wash: treatment technologies and potential applications. J Hazard Mater 163:12–25

    Article  PubMed  CAS  Google Scholar 

  • Pant D, Adholeya A (2007a) Biological approaches for treatment of distillery wastewater: a review. Bioresour Technol 98:2321–2334

    Article  PubMed  CAS  Google Scholar 

  • Pant D, Adholeya A (2007b) Enhanced production of ligninolytic enzymes and decolorization of molasses distillery wastewater by fungi under solid state fermentation. Biodegradation 18:647–659

    Article  PubMed  CAS  Google Scholar 

  • Pant D, Adholeya A (2009) Nitrogen removal from biomethanated spentwash using hydroponic treatment followed by fungal decolourization. Environ Eng Sci 26:559–565

    Article  CAS  Google Scholar 

  • Pant D, Adholeya A (2010) Development of a novel fungal consortium for the treatment of molasses distillery wastewater. Environmentalist 30:178–182

    Article  Google Scholar 

  • Papagianni M (2004) Fungal morphology and metabolite production in submerged mycelial processes. Biotechnol Adv 22:189–259

    Article  PubMed  CAS  Google Scholar 

  • Raghukumar C, Mohandass C, Kamat S, Shailaja MS (2004) Simultaneous detoxification and decolourization of molasses spent wash by the immobilized white-rot fungus Flavodon flavus isolated from a marine habitat. Enzyme Microb Technol 35:197–202

    Article  CAS  Google Scholar 

  • Satyawali Y, Balakrishnan M (2007) Wastewater treatment in molasses-based alcohol distilleries for COD and color removal: a review. J Environ Manage 86:481–497

    Article  PubMed  Google Scholar 

  • Shayegan J, Pazouki M, Afshari A (2005) Continuous decolourization of anaerobically digested distillery wastewater. Process Biochem 40:1323–1329

    Article  CAS  Google Scholar 

  • Shuler M, Kargi F (2002) Bioprocess engineering, basic concepts, 2nd edn. Prentice Hall, Inc., New Jersey

    Google Scholar 

  • Singh SS, Dikshit AK (2010) Optimization of the parameters for decolourization by Aspergillus niger of anaerobically digested distillery spentwash pretreated with polyaluminium chloride. J Hazard Mater 176:864–869

    Article  PubMed  CAS  Google Scholar 

  • Thiruchelvam AT, Ramsay JA (2007) Growth and laccase production kinetics of Trametes versicolor in a stirred tank reactor. Appl Microbiol Biotechnol 74:547–554

    Article  PubMed  CAS  Google Scholar 

  • Watanabe Y, Sugi R, Tanaka Y, Hayashida S (1982) Enzymatic decolourization of melanoidin by Coriolus sp. Agric Biol Chem 46:1623–1630

    Article  CAS  Google Scholar 

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  1. A. K. Dikshit

    Present address: Department of Civil and Environmental Engineering, Nanyang Technological University, Singapore, 639798, Singapore

Authors and Affiliations

  1. Centre for Environmental Science and Engineering, Indian Institute of Technology, Bombay, Powai, Mumbai, 400 076, India

    S. S. Singh & A. K. Dikshit

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Correspondence to A. K. Dikshit.

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Singh, S.S., Dikshit, A.K. Decolourization of anaerobically digested and polyaluminium chloride treated distillery spentwash in a fungal stirred tank aerobic reactor. Biodegradation 22, 1109–1117 (2011). https://doi.org/10.1007/s10532-011-9467-z

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  • DOI: https://doi.org/10.1007/s10532-011-9467-z

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