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Biodegradation of polyfluorinated biphenyl in bacteria

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Abstract

Fluorinated aromatic compounds are significant environmental pollutants, and microorganisms play important roles in their biodegradation. The effect of fluorine substitution on the transformation of fluorobiphenyl in two bacteria was investigated. Pseudomonas pseudoalcaligenes KF707 and Burkholderia xenovorans LB400 used 2,3,4,5,6-pentafluorobiphenyl and 4,4′-difluorobiphenyl as sole sources of carbon and energy. The catabolism of the fluorinated compounds was examined by gas chromatography–mass spectrometry and fluorine-19 nuclear magnetic resonance spectroscopy (19F NMR), and revealed that the bacteria employed the upper pathway of biphenyl catabolism to degrade these xenobiotics. The novel fluorometabolites 3-pentafluorophenyl-cyclohexa-3,5-diene-1,2-diol and 3-pentafluorophenyl-benzene-1,2-diol were detected in the supernatants of biphenyl-grown resting cells incubated with 2,3,4,5,6-pentafluorobiphenyl, most likely as a consequence of the actions of BphA and BphB. 4-Fluorobenzoate was detected in cultures incubated with 4,4′-difluorobiphenyl and 19F NMR analysis of the supernatant from P. pseudoalcaligenes KF707 revealed the presence of additional water-soluble fluorometabolites.

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Acknowledgments

The authors acknowledge financial support from Enterprise Ireland. We thank Jennifer Power for assistance with the resting cell experiments.

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Authors and Affiliations

  1. School of Biomolecular and Biomedical Science and Centre for Synthesis and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland

    David Hughes, Benjamin R. Clark & Cormac D. Murphy

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  1. David Hughes
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  2. Benjamin R. Clark
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  3. Cormac D. Murphy
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Correspondence to Cormac D. Murphy.

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Hughes, D., Clark, B.R. & Murphy, C.D. Biodegradation of polyfluorinated biphenyl in bacteria. Biodegradation 22, 741–749 (2011). https://doi.org/10.1007/s10532-010-9411-7

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  • DOI: https://doi.org/10.1007/s10532-010-9411-7

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