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Isolation and characterization of an abamectin-degrading Burkholderia cepacia-like GB-01 strain

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Abstract

Abamectin is widely used in agriculture as an insecticide and in veterinary as an anti-parasitic agent, and has caused great environmental pollution by posing potential risk to non-target soil invertebrates and nearby aquatic systems. A bacterium designated GB-01, which was capable of degrading abamectin, was isolated from soil by enrichment culture method. On the basis of morphological, physiological and biochemical characteristics, combined with phylogenetic analysis of 16S rRNA gene, the bacterium GB-01 was identified as Burkholderia cepacia-like species. The bacterium GB-01 was able to utilize abamectin as its sole carbon source for growth, and could degrade more than 90% of abamectin at initial concentrations of 50 and 100 mg l−1 in mineral salt medium in 30 and 36 h, respectively. The longer degradation cycle was observed with abamectin concentrations higher than 100 mg l−1. Optimal growth temperatures and pH values with highest degradation rate were 30–35°C and 7–8, respectively. Two new degradation products were identified and characterized by high performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) based mass spectral data and a plausible partial degradation pathway of abamectin was proposed. This is the first report in which an abamectin-degrading Burkholderia species isolated from soil was identified and characterized.

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Acknowledgments

This research work was partially supported by China Natural Science and Technology Resources Platform Program (2005DKA21201-2). Shinawar Waseem Ali was financially supported by Higher Education Commission (HEC) Pakistan for studies in China (2005PKC037).

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Authors and Affiliations

  1. Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, China

    Shinawar Waseem Ali, Rong Li, Ji-quan Sun, Peng Guo, Ji-ping Ma & Shun-peng Li

  2. School of Chemical Engineering, Nanjing University of Science and Technology, 210094, Nanjing, Jiangsu Province, China

    Wei-you Zhou

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  1. Shinawar Waseem Ali
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  2. Rong Li
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Correspondence to Shun-peng Li.

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Ali, S.W., Li, R., Zhou, Wy. et al. Isolation and characterization of an abamectin-degrading Burkholderia cepacia-like GB-01 strain. Biodegradation 21, 441–452 (2010). https://doi.org/10.1007/s10532-009-9314-7

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