Abstract
In the presence of a redox mediator, 1-hydroxybenzotriazole (HBT), iso-butylparaben (iso-BP) and n-butylparaben (n-BP) were treated with laccase from white rot fungus Trametes versicolor. HPLC analysis demonstrated that iso-BP and n-BP almost completely disappeared from the reaction mixture after 4 h of treatment with the laccase-HBT system. Using the yeast two-hybrid assay system, it was also confirmed that the laccase-HBT system substantially removed the estrogenic activity of iso-BP and n-BP after 4 h of treatment. Furthermore, there was a linear relationship between the removal of estrogenic activity of both parabens and the decrease in their concentrations. These results demonstrate that the laccase-HBT system is effective in eliminating iso-BP and n-BP, and removing the estrogenic activity of both parabens.
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Acknowledgments
This research was supported in part by a Grant-in-Aid for Scientific Research, Japan (20580173). We would like to thank Dr. T. Nishihara, Professor Emeritus, of Osaka University for providing the yeast two-hybrid system for assay of estrogenic activity.
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Mizuno, H., Hirai, H., Kawai, S. et al. Removal of estrogenic activity of iso-butylparaben and n-butylparaben by laccase in the presence of 1-hydroxybenzotriazole. Biodegradation 20, 533–539 (2009). https://doi.org/10.1007/s10532-008-9242-y
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DOI: https://doi.org/10.1007/s10532-008-9242-y