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Identification and characterization of chlorpyrifos-methyl and 3,5,6-trichloro-2-pyridinol degrading Burkholderia sp. strain KR100

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Abstract

A chlorpyrifos-methyl (CM) degrading bacterium (designated strain KR100) was isolated from a Korean rice paddy soil and was further tested for its sensitivity against eight commercial antibiotics. Based on morphological, biochemical, and molecular characteristics, this bacterium showed greatest similarity to members of the order Burkholderiales and was shown to be most closely related to members of the Burkholderia cepacia group. Strain KR100 hydrolyzed CM to 3,5,6-trichloro-2-pyridinol (TCP) and utilized TCP as the sole source of carbon for its growth. The isolate was also able to degrade chlorpyrifos, dimethoate, fenitrothion, malathion, and monocrotophos at 300 μg/ml but diazinon, dicrotophos, parathion, and parathion-methyl at 100 μg/ml. The ability to degrade CM was found to be encoded on a single plasmid of ~50 kb, pKR1. Genes encoding resistance to amphotericin B, polymixin B sulfate, and tetracycline were also located on the plasmid. This bacterium merits further study as a potential biological agent for the remediation of soil, water, or crop contaminated with organophosphorus compounds because of its greater biodegradation activity and its broad specificity against a range of organophosphorus insecticides.

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Abbreviations

CM:

Chlorpyrifos-methyl

TCP:

3,5,6-Trichloro-2-pyridinol

OP:

Organophosphorus insecticide

MSM:

Mineral salts basal medium

PTYG:

Peptone–tryptone–yeast extract–glucose

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Acknowledgments

This work was supported by grants from NaturoBiotech Co., Ltd and the Ministry of Education and Human Resources Development for Brain Korea 21 Project of the Korean Government to YJA.

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  1. Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921, Republic of Korea

    Jun-Ran Kim & Young-Joon Ahn

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  1. Jun-Ran Kim
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Correspondence to Young-Joon Ahn.

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Kim, JR., Ahn, YJ. Identification and characterization of chlorpyrifos-methyl and 3,5,6-trichloro-2-pyridinol degrading Burkholderia sp. strain KR100. Biodegradation 20, 487–497 (2009). https://doi.org/10.1007/s10532-008-9238-7

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