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Effect of fluctuations in salinity on anaerobic biomass and production of soluble microbial products (SMPs)

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Abstract

This study investigated the acclimation potential of batch fed anaerobic biomass with salinities of 0–50 gNaCl l−1. Anaerobic biomass was acclimatized to salinities up to 20 gNaCl l−1over a period of 35 days, with 3 consecutive feedings. After this period the biomass was subjected to non-saline conditions to simulate fluctuating feed compositions. High activity was obtained after the first exposure to non saline conditions for biomass previously exposed to 30 gNaCl l−1. Short exposure (2–48 h) to high salinity (40 gNaCl l−1) did not reduce biomass activity when it was re-subjected to normal conditions. The sensitivity of each anaerobic bacterial group showed that propionate utilisers were the most affected by sudden changes in salinity. Using size exclusion chromatography (SEC) it was found that biomass exposed to concentrations of salinity above 30 gNaCl−1 produced higher molecular weight soluble microbial products (SMPs) which were present in the culture for longer periods than the control indicating that the effluent was more difficult to degrade. With the sudden removal of salinity anaerobic biomass can easily readapt to normal conditions without any high MW compounds being produced. These findings highlight the fact that anaerobic biomass is able to overcome sharp decreases in salinity in contrast with aerobic biomass as reported in the literature.

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References

  • APHA (1999) Standard methods for the examination of water and wastewater. American Public Health Association, Washington, D.C

    Google Scholar 

  • Aquino SF, Stuckey DC (2006) Chromatographic characterization of dissolved organics in effluents from two anaerobic reactors treating synthetic wastewater. Water Sci Technol 54(2):193–198. doi:10.2166/wst.2006.505

    Article  PubMed  CAS  Google Scholar 

  • Aquino SF, Hu AY, Akram A, Stuckey DC (2006) Characterization of dissolved compounds in submerged anaerobic membrane bioreactors (SAMBRs). J Chem Technol Biotechnol 81(12):1894–1904. doi:10.1002/jctb.1622

    Article  CAS  Google Scholar 

  • Barker DJ, Stuckey DC (1999) A review of soluble microbial products (SMP) in wastewater treatment systems. Water Res 33(14):3063–3082. doi:10.1016/S0043-1354(99)00022-6

    Article  CAS  Google Scholar 

  • Boardman GD, Tisinger JL, Gallagher DL (1995) Treatment of clam processing wastewaters by means of upflow anaerobic sludge blanket technology. Water Res 29(6):1483–1490

    Article  CAS  Google Scholar 

  • Burnett WE (1974) The effect of salinity variations on the activated sludge process. Water Sew Works 121:37–38

    CAS  Google Scholar 

  • Debaere LA, Devocht M, Vanassche P, Verstraete W (1984) Influence of high NaCl and NH4Cl salt levels on methanogenic associations. Water Res 18(5):543–548. doi:10.1016/0043-1354(84)90201-X

    Article  CAS  Google Scholar 

  • Dolfing J, Bloemen W (1985) Activity measurements as a tool to characterize the microbial composition of methanogenic environments. J Microbiol Methods 4(1):1–12. doi:10.1016/0167-7012(85)90002-8

    Article  CAS  Google Scholar 

  • Feijoo G, Soto M, Mendez R, Lema JM (1995) Sodium inhibition in the anaerobic-digestion process—antagonism and adaptation phenomena. Enzyme Microb Technol 17(2):180–188. doi:10.1016/0141-0229(94)00011-F

    Article  CAS  Google Scholar 

  • Kargi F, Dincer AR (1998) Saline wastewater treatment by halophile-supplemented activated sludge culture in an aerated rotating biodisc contactor. Enzyme Microb Technol 22(6):427–433. doi:10.1016/S0141-0229(97)00215-9

    Article  CAS  Google Scholar 

  • Kempf B, Bremer E (1998) Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch Microbiol 170(5):319–330. doi:10.1007/s002030050649

    Article  PubMed  CAS  Google Scholar 

  • Kinncannon DF, Gaudy AF (1966) Some effect of high salt concentrations on activated sludge. J Water Pollut Control Fed 38:1148–1156

    Google Scholar 

  • Kinncannon DF, Gaudy AF (1968) Response of biological waste treatment systems to change in salt concentrations. Biotechnol Bioeng 10:483–496. doi:10.1002/bit.260100408

    Article  Google Scholar 

  • Kugelman IJ, McCarty PL (1965) Cation toxicity and stimulation in anaerobic waste treatment. J Water Pollut Control Fed 37:97–116

    CAS  Google Scholar 

  • Lefebvre O, Moletta R (2006) Treatment of organic pollution in industrial saline wastewater: a literature review. Water Res 40(20):3671–3682. doi:10.1016/j.watres.2006.08.027

    Article  PubMed  CAS  Google Scholar 

  • Liu YT, Boone DR (1991) Effects of salinity on methanogenic decomposition. Bioresour Technol 35(3):271–273. doi:10.1016/0960-8524(91)90124-3

    Article  CAS  Google Scholar 

  • Mendez R, Lema JM, Soto M (1995) Treatment of seafood-processing wastewaters in mesophilic and thermophilic anaerobic filters. Water Environ Res 67(1):33–45. doi:10.2175/106143095X131178

    Article  CAS  Google Scholar 

  • Owen WF, Stuckey DC, Healy JB, Young LY, McCarty PL (1979) Bioassay for monitoring biochemical methane potential and anaerobic toxicity. Water Res 13(6):485–492. doi:10.1016/0043-1354(79)90043-5

    Article  CAS  Google Scholar 

  • Rovirosa N, Sanchez E, Cruz M, Veiga MC, Borja R (2004) Coliform concentration reduction and related performance evaluation of a down-flow anaerobic fixed bed reactor treating low-strength saline wastewater. Bioresour Technol 94(2):119–127. doi:10.1016/j.biortech.2003.12.010

    Article  PubMed  CAS  Google Scholar 

  • Soto M, Mendez R, Lema JM (1993) Sodium inhibition and sulfate reduction in the anaerobic treatment of mussel processing wastewaters. J Chem Technol Biotechnol 58(1):1–7

    CAS  Google Scholar 

  • Speece RE (1996) Anaerobic biotechnology for industrial wastewater. Archaea Press, Nashville, Tennessee

    Google Scholar 

  • Tuin BJW, Geerts R, Westerink JB, van Ginkel CG (2006) Pretreatment and biotreatment of saline industrial wastewaters. Water Sci Technol 53(3):17–25. doi:10.2166/wst.2006.072

    Article  PubMed  CAS  Google Scholar 

  • Vyrides I, Stuckey DC (2007) Mechanisms employed by anaerobic biomass to survive under high saline conditions: compatible solutes. 11th world congress on anaerobic digestion, Brisbane, Australia

  • Zheng XJ, Zheng YM, Yu HQ (2005) Influence of NaCl on hydrogen production from glucose by anaerobic cultures. Environ Technol 26(10):1073–1080

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

I.V. would like to thank the A.G. Leventis Foundation for the award of a scholarship.

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  1. Department of Chemical Engineering and Chemical Technology, Imperial College London, South Kensington, London, SW7 2AZ, UK

    I. Vyrides & D. C. Stuckey

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  1. I. Vyrides
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  2. D. C. Stuckey
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Correspondence to D. C. Stuckey.

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Vyrides, I., Stuckey, D.C. Effect of fluctuations in salinity on anaerobic biomass and production of soluble microbial products (SMPs). Biodegradation 20, 165–175 (2009). https://doi.org/10.1007/s10532-008-9210-6

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