Abstract
In this study we used the assimilation of isotope labeled CO2 to measure the substrate preferences by two different bioaugmentation mixtures proposed for bioremediation of diesel oil contamination. All active microorganisms assimilate CO2 in various carboxylation processes involved in growth. The CO2 assimilation by the two mixtures was measured upon addition of glucose, diesel oil or specific compounds present in diesel oil (naphthalene, toluene, hexadecane, and octane). It was shown that within short term incubations with diesel oil (<5 h), one bioaugmentation mixture was superior to the other regarding the assimilation of CO2. This observation was confirmed in a labor-intensive long term microcosm study (60 days). The applied method open various possibilities for fast pre-testing of substrate-preferences by microbial-bioaugmentation mixtures without microcosm experiments, onsite tests, and complicated chemical analysis. This study also demonstrates the possibility to obtain further information on the substrate preferences at a single cell level of phylogenetically defined microbial subgroups in bioaugmentation mixtures, based on combined analyses of microautoradiography and fluorescence in situ hybridization.
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Abbreviations
- AI:
-
CO2 assimilation Index
- BS and JR:
-
Microbial-bioaugmentation mixtures tested in this study (see material and methods)
- FISH:
-
Fluorescence in situ hybridization
- HetCO2-MAR:
-
Microautoradiography of heterotrophic bacteria on the basis of assimilation of 14CO2
- MAR:
-
Microautoradiography
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Acknowledgements
The principal funding for this study was provided by Danish Technical Research Council (grants # 26-03-0036 to MH and #26-03-02250 to JLN). Additional generous private support to MH from the R98-foundation is highly appreciated. Maria Grøn and Pia Jensen are acknowledged for their contribution to the microcosm work (M.Sc. thesis at the Department of biotechnology, chemistry and environmental engineering, Aalborg University).
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Hesselsoe, M., Bjerring, M.L., Henriksen, K. et al. Method for measuring substrate preferences by individual members of microbial consortia proposed for bioaugmentation. Biodegradation 19, 621–633 (2008). https://doi.org/10.1007/s10532-007-9167-x
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DOI: https://doi.org/10.1007/s10532-007-9167-x