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Bioremediation of polycyclic aromatic hydrocarbons contaminated soil with Monilinia sp.: degradation and microbial community analysis

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Abstract

Microcosms were set up with a PAHs-contaminated soil using biostimulation (addition of ground corn cob) and bioaugmentation (inoculated with Monilinia sp. W5-2). Degradation of polycyclic aromatic hydrocarbons and microbial community were examined at the end of incubation period. After 30 days, bioaugmented microcosms showed a 35 ± 0% decrease in total PAHs, while biostimulated and control microcosms showed 16 ± 9% and 3 ± 0% decrease in total PAHs, respectively. Bioaugmented microcosms also revealed 70 ± 8% and 72 ± 2% decreases in benzo[a]pyrene and anthracene, respectively, while the values for biostimulated and control microcosms were much lower. Detoxification of soils in bioaugmented microcosms was confirmed by genetic toxicity assay, suggesting important role of fungal remediation. Molecular fingerprint profiles and selective enumeration showed biostimulation with ground corn cob increased both number and abundance of indigenous aromatic hydrocarbons degraders and changed microbial community composition in soil, which is beneficial to natural attenuation of PAHs. At the same time, bioaugmentation with Monilinia strain W5-2 imposed negligible effect on indigenous microbial community. This study suggests that fungal remediation is promising in eliminating PAHs, especially the part of recalcitrant and highly toxic benzo[a]pyrene, in contaminated soil. It is also the first description of soil bioremediation with Monilinia sp.

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Acknowledgments

This research was funded by grants from Ministry of Science and Technology of the People’s Republic of China (2002CB410809) and National Natural Science Foundation of China (40432005). We thank Dr. Y. Oda, Osaka Prefectural Institute of Public Health (Osaka, Japan) for kindly supplying S. typhimurium NM2009. We also thank Prof. Yunlong Yu, Zhejiang University (Hangzhou, China) for help in identifying fungus W5-2.

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Authors and Affiliations

  1. Soil and Environmental Bioremediation Research Center, State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Yucheng Wu, Yongming Luo, Dexun Zou, Jinzhi Ni, Wuxin Liu, Ying Teng & Zhengao Li

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China

    Yucheng Wu & Yongming Luo

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  1. Yucheng Wu
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  2. Yongming Luo
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  3. Dexun Zou
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  4. Jinzhi Ni
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  5. Wuxin Liu
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  7. Zhengao Li
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Correspondence to Yongming Luo.

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Wu, Y., Luo, Y., Zou, D. et al. Bioremediation of polycyclic aromatic hydrocarbons contaminated soil with Monilinia sp.: degradation and microbial community analysis. Biodegradation 19, 247–257 (2008). https://doi.org/10.1007/s10532-007-9131-9

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  • DOI: https://doi.org/10.1007/s10532-007-9131-9

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