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Isolation and characterization of phenanthrene-degrading Sphingomonas paucimobilis strain ZX4

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Abstract

Phenanthrene-degrading bacterium strain ZX4 was isolated from an oil-contaminated soil, and identified as Sphingomonas paucimobilis based on 16S rDNA sequence, cellular fatty acid composition, mol% G + C and Biolog-GN tests. Besides phenanthrene, strain ZX4 could also utilize naphthalene, fluorene and other aromatic compounds. The growth on salicylic acid and catechol showed that the strain degraded phenanthrene via salicylate pathway, while the assay of catechol 2, 3-dioxygenase revealed catechol could be metabolized through meta-cleavage pathway. Three genes, including two of meta-cleavage operon genes and one of GST encoding gene were obtained. The order of genes arrangement was similar to S-type meta-pathway operons. The phylogenetic trees based on 16S rDNA sequence and meta-pathway gene both revealed that strain ZX4 is clustered with strains from genus Sphingomonas.

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou, 310029, China

    Ying Xia, Hang Min, Zhen-mei Lv, Ji Liu, Yang-fang Ye & Xue-jun Duan

  2. Microbiology Institute, Chinese Academy of Sciences, Beijing, 100080, China

    Ying Xia

  3. State Intellectual Property Office of P.R. China, Beijing, 100080, China

    Gang Rao

Authors
  1. Ying Xia
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  2. Hang Min
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  3. Gang Rao
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  4. Zhen-mei Lv
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  5. Ji Liu
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  6. Yang-fang Ye
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  7. Xue-jun Duan
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Correspondence to Hang Min.

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Xia, Y., Min, H., Rao, G. et al. Isolation and characterization of phenanthrene-degrading Sphingomonas paucimobilis strain ZX4. Biodegradation 16, 393–402 (2005). https://doi.org/10.1007/s10532-004-2412-7

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  • DOI: https://doi.org/10.1007/s10532-004-2412-7

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