A molecular marker approach was used to analyse the genetic diversity of endemic Triticum aethiopicum wheat accessions that were collected from the same sites in Ethiopia by N.I. Vavilov in 1927 and by the Joint Ethiopian-Russian Biological Expedition in 2012, which allowed the assessment of changes in the gene pool structure of this species over 85 years. The level of genetic diversity in the accessions from Vavilov’s collection was higher than that of the accessions collected in 2012. Analysis of molecular variance indicated that differentiation between the two groups of accessions was 17% (PhiPT = 0.169; p < 0.010). A Bayesian clustering approach and principal coordinates analysis showed that these two groups of accessions were notably different in their genetics. In this regard, this article discusses the problem of in situ and ex situ conservation of wheat landraces: their evolution, stability and genetic drift.
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Species assignment of the accessions collected by the expedition of 2012 was carried out at the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences (Novosibirsk, Russia), within the framework of the state contract no. 0324-2019-0039-C-01. Molecular analysis was conducted at the Vavilov Institute of General Genetics, Russian Academy of Sciences (Moscow, Russia) within the framework of the state contract of this institute. The material was collected in 2012 within the framework of the long-term international scientific project’s Joint Ethiopian-Russian Biological Expedition (JERBE) organised by the Division of Biological Sciences of the Russian Academy of Sciences. We thank the JERBE coordinator A.A. Darkov (A.N. Severtsov Institute of Ecology and Evolution, RAS, Moscow, Russia) for his help in organising the field studies. We thank Prof. O.P. Mitrofanova and N.S. Lysenko (VIR, St. Petersburg, Russia) for providing accessions of N.I. Vavilov’s Ethiopian expedition and Drs. T.N. Smekalova and L.V. Bagmet (VIR, St. Petersburg, Russia) for the opportunity to work with herbarium sheets of those accessions. We thank E.Ya. Kondratenko (Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia) for technical assistance.
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This article belongs to the Topical Collection: Ex-situ conservation.
Communicated by Daniel Sanchez Mata.
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Trifonova, A.A., Dedova, L.V., Zuev, E.V. et al. Comparative analysis of the gene pool structure of Triticum aethiopicum wheat accessions conserved ex situ and recollected in fields after 85 years. Biodivers Conserv (2021). https://doi.org/10.1007/s10531-020-02091-6
- Triticum aethiopicum
- AFLP analysis