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Plant agro-biodiversity needs protection, study and promotion: results of research conducted in Lombardy region (Northern Italy)

  • Luca GiupponiEmail author
  • Roberto Pilu
  • Alessio Scarafoni
  • Annamaria Giorgi
Original Paper
Part of the following topical collections:
  1. Biodiversity exploitation and use

Abstract

The loss of plant agro-biodiversity is a global problem with repercussions on both humans and (agro-)ecosystems. This article presents the data of a census of the herbaceous landraces currently cultivated in Lombardy (Northern Italy), one of the most industrialized regions of Europe, and for which information was previously extremely limited. The census showed that 72 herbaceous landraces are cultivated (conserved on farms) in Lombardy yet most of them are threatened since they are cultivated by a small number of farmers, mostly hobbyists. Only 11% have been the subject of scientific studies while 12.5% are protected since they are registered in the European Register of Conservation Varieties. Lombardy has lost about 78% of its landraces cultivated over the last 70–80 years. The nutritional characteristics of four little-known maize landraces of the Lombardy region recently used for the creation of niche food chains were also analyzed. They have a higher content of protein (about 12.34%) and phytic acid (about 1.35%), compared to a hybrid maize (B73/Mo17), while they are slightly poorer in starch (about 77.85%), Mg and Zn. Some of these landraces, those with coloured kernels due to the high concentration of polyphenols, have high antioxidant activity which makes them interesting for the production of nutraceutical foods. 2D-electrophoretic protein profiles highlighted that the four maize landraces are different one from another. Finally, some actions and tools are suggested to favour the in situ conservation of plant agro-biodiversity.

Keywords

Agricultural biodiversity In situ conservation Landraces Nutritional value Plant genetic resources Zea mays 

Notes

Acknowledgments

We wish to thank Elisabetta Galanti, Arianna Di Marco, Lara Domini, Davide Maria Molinari for their work in the field and in the laboratory, Laura Ronchi of Lombardy Region for her support, and all the farmers and associations that have contributed to this study. This research was supported by “Accordo di collaborazione fra Regione Lombardia e CRC Ge.S.Di.Mont. per attività di ricerca scientifica ed applicata e di diffusione della conoscenza inerente il territorio montano lombardo (art. 4 c.7 l.r. 22/2016)”, “FISR-MIUR Italian Mountain Lab” project and by “Valorizzazione della biodiversità dei territori di montagna, con particolare riferimento all’agro-biodiversità e derivati: orizzonti di bio e green economy per la montagna (DARA–CRC Ge.S.Di.Mont.)” project.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10531_2019_1889_MOESM1_ESM.xlsx (36 kb)
Supplementary material 1 (XLSX 35 kb)

References

  1. Al-Turki TA, Al-Namazi AA, Masrahi YS (2018) Conservation of genetic resources for five traditional crops from Jazan, SW Saudi Arabia, at the KACST Gene-Bank. Saudi J Biol Sci.  https://doi.org/10.1016/j.sjbs.2018.09.007 CrossRefGoogle Scholar
  2. Anderson TJ, Lamsal BP (2011) Zein extraction from corn, corn products, and coproducts and modifications for various applications: a review. Cereal Chem 88:159–173CrossRefGoogle Scholar
  3. AOAC (2000) Official methods of analysis of AOAC. International 17th edition. Association of Analytical Communities, Gaithersburg, p 43–44Google Scholar
  4. Barcaccia G, Volpato M, Gentili R, Abeli T, Galla G, Orsenigo S, Citterio S, Sgorbati S, Rossi G (2016) Genetic identity of common buckwheat (Fagopyrum esculentum Moench) landraces locally cultivated in the Alps. Genet Resour Crop Evol 63:639–651CrossRefGoogle Scholar
  5. Bertolini M (2002) Mais in Lombardia: varietà tradizionali. Istituto Sperimentale per la Cerealicoltura Sezione di Bergamo, Quaderni della RicercaGoogle Scholar
  6. Bragdon S (1996) The convention on biological diversity. Glob Environ Change 6:177–179CrossRefGoogle Scholar
  7. Camacho Villa TC, Maxted N, Scholten M, Ford-Lloyd B (2005) Defining and identifying crop landraces. Plant Genet Res 3:373–384CrossRefGoogle Scholar
  8. Capraro J, Magni C, Giorgi A, Duranti M, Scarafoni A (2018) Comparative 1D- and 2D-electrophoretic protein profiles of ancestral and modern buckwheat seeds grown in the Italian Alpine Region. Ital J Food Sci 30:497–503Google Scholar
  9. Cassani E, Puglisi D, Cantaluppi E, Landoni M, Giupponi L, Giorgi A, Pilu R (2017) Genetic studies regarding the control of seed pigmentation of an ancient European pointed maize (Zea mays) rich in phlobaphenes: the “Nero Spinoso” from the Camonica valley. Genet Resour Crop Evol 64:761–773CrossRefGoogle Scholar
  10. CBD [Convention on Biological Diversity] (1992) Convention on biological diversity: text and annexes. Secretariat of the Convention on Biological Diversity, Montreal https://www.cbd.int/convention/text/. Accessed 11 April 2019
  11. CBD [Convention on Biological Diversity] (2002) Global strategy for plant conservation. Secretariat of the Convention on Biological Diversity, Montreal. https://www.cbd.int/gspc/documents.shtml. Accessed 11 April 2019
  12. CBD [Convention on Biological Diversity] (2010) Conference of the parties 10 decision X/17. Consolidated Update of the Global Strategy for Plant Conservation 2011–2020. Secretariat of the Convention on Biological Diversity, Montreal. https://www.cbd.int/decision/cop/?id=12283. Accessed 11 April 2019
  13. Ceccarelli S (2012) Landraces: importance and use in breeding and environmentally friendly agronomic systems. In: Maxted et al (eds) Agrobiodiversity conservation: securing the diversity of crop wild relatives and landraces. CAB International, Oxfordshire, pp 103–117CrossRefGoogle Scholar
  14. Cleveland DA, Soleri D, Smith SE (1994) Do folk crop varieties have a role in sustainable agriculture? Bioscience 11:740–751CrossRefGoogle Scholar
  15. Directive 2008/62/EC (2008) Commission Directive 2008/62/EC of 20 June 2008 providing for certain derogations for acceptance of agricultural landraces and varieties which are naturally adapted to the local and regional conditions and threatened by genetic erosion and for marketing of seed and seed potatoes of those landraces and varieties. https://eur-lex.europa.eu/legal-content/EN/ALL/?uri=CELEX%3A32008L0062. Accessed 01 September 2019
  16. Directive 2009/145/EC (2009) Commission Directive 2009/145/EC of 26 November 2009 providing for certain derogations, for acceptance of vegetable landraces and varieties which have been traditionally grown in particular localities and regions and are threatened by genetic erosion and of vegetable varieties with no intrinsic value for commercial crop production but developed for growing under particular conditions and for marketing of seed of those landraces and varieties. https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX%3A32009L0145. Accessed 01 September 2019
  17. Directive 2010/60/EU (2010) Commission Directive 2010/60/EU of 30 August 2010 providing for certain derogations for marketing of fodder plant seed mixtures intended for use in the preservation of the natural environment Text with EEA relevance. https://eur-lex.europa.eu/legal-content/EN/TXT/?qid=1567082342503&uri=CELEX:32010L0060. Accessed 01 September 2019
  18. Eichten SR, Foerster JM, de Leon N, Kay Y, Yeh CT, Liu S, Jeddeloh JA, Schnable PS, Kaeppler SM, Springer NM (2011) B73-Mo17 near-isogenic lines demonstrate dispersed structural variation in maize. Plant Physiol 156:1679–1690PubMedPubMedCentralCrossRefGoogle Scholar
  19. Esquinas-Alcázar J (2010) Protecting crop genetic diversity for food security: political, ethical and technical challenges. Nature 6:946–953Google Scholar
  20. EU Commission (2010) EUROPE 2020 A strategy for smart, sustainable and inclusive growth. https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX%3A52010DC2020. Accessed 11 April 2019
  21. EU Commission (2017) Region innovation monitor plus. https://ec.europa.eu/growth/tools-databases/regional-innovation-monitor/base-profile/lombardy. Accessed 11 April 2019
  22. FAO (1999) Agricultural biodiversity. Background Paper 1. FAO/Netherlands Conference of multifunctional character of agriculture and land. http://www.fao.org/mfcal/pdf/bp_1_agb.pdf. Accessed 11 April 2019
  23. FAO (2004) Building on gender, Agrobiodiversity and local Knowledge. Food and agriculture organization of the United Nations, Rome. http://www.fao.org/docrep/007/y5609e/y5609e00.htm. Accessed 11 April 2019
  24. FAO (2009) International treaty on plant genetic resources for food and agriculture. Food and agriculture Organization of the United Nations, Rome. http://www.fao.org/3/a-i0510e.pdf. Accessed 11 April 2019
  25. FAO (2010) Second report on the state of the world’s plant genetic resources for food and agriculture. Commission on Genetic Resources for Food and Agriculture. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
  26. Fideghelli C, Engel P (2009) Biodiversity and local genetic resources: from knowledge to exploitation. Acta Hortic 817:295–310CrossRefGoogle Scholar
  27. Frankel OH, Brown AHD, Burdon JJ (1995) Conservation of plant biodiversity. Cambridge University Press, UKGoogle Scholar
  28. Frei M, Becker K (2004) Agro-biodiversity in subsistence-oriented farming systems in a Philippine upland region: nutritional considerations. Biodivers Conserv 13:1591–1610CrossRefGoogle Scholar
  29. Frison EA, Cherfas J, Hodgkin T (2011) Agricultural biodiversity is essential for a sustainable improvement in food and nutrition security. Sustainability 3:238–253CrossRefGoogle Scholar
  30. Giacomini V (1954) Il grano siberiano (Fagopyrum tataricum) in Valtellina. Ramponi, SondrioGoogle Scholar
  31. Giorgi A, Scheurer T (2015) Alpine resources: assets for a promising future. Conclusions from the Forum Alpinum 2014. Mt Res Dev 35:414–415CrossRefGoogle Scholar
  32. Giupponi L, Corti C, Manfredi P, Cassinari C (2013) Application of the oristic-vegetational indexes system for the evaluation of the environmental quality of a semi-natural area of the Po Valley (Piacenza, Italy). Plant Sociol 50:47–56Google Scholar
  33. Giupponi L, Corti C, Manfredi P (2015) The vegetation of the Borgotrebbia landfill (Piacenza, Italy): phytosociological and ecological characteristics. Plant Biosyst 149:865–874CrossRefGoogle Scholar
  34. Giupponi L, Tamburini A, Giorgi A (2018) Prospects for broader cultivation and commercialization of Copafam, a local variety of Phaseolus coccineus, in the Brescia Pre-Alps. Mt Res Dev 38:24–34CrossRefGoogle Scholar
  35. Giupponi L, Borgonovo G, Panseri S, Giorgi A (2019) Multidisciplinary study of a little known landrace of Fagopyrum tataricum Gaertn. of Valtellina (Italian Alps). Genet Resour Crop Evol 66:783–796CrossRefGoogle Scholar
  36. Gupta RK, Gangoliya SS, Singhcorresponding NK (2015) Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains. J Food Sci Technol 52:676–684PubMedCrossRefPubMedCentralGoogle Scholar
  37. Hammer K, Knüppfer H, Xhuveli L, Perrino P (1996) Estimating genetic erosion in landraces—two case studies. Genet Resour Crop Evol 43:329–336CrossRefGoogle Scholar
  38. Hammer K, Gladis T, Diederichsen A (2003) In situ and on-farm management of plant genetic resources. Eur J Agron 19:509–517CrossRefGoogle Scholar
  39. Hammer K, Montesano V, Direnzo P, Laghetti G (2018) Conservation of crop genetic resources in Italy with a focus on vegetables and a case study of a neglected race of Brassica oleracea. Agriculture 8:105CrossRefGoogle Scholar
  40. ISMEA (2014) Stime di produzione—Cereali autunnali, mais, semi oleosi. http://www.ismeamercati.it/flex/cm/pages/ServeBLOB.php/L/IT/IDPagina/4292. Accessed 11 April 2019
  41. ISTAT (2010) Censimento Agricoltura 2010. http://dati-censimentoagricoltura.istat.it/Index.aspx. Accessed 11 April 2019
  42. IUCN (2019) The IUCN red list of threatened species. Version 2019-1. http://dati-censimentoagricoltura.istat.it/Index.aspxhttps://www.iucnredlist.org. Accessed 11 April 2019
  43. Jackson LE, Pascual U, Hodgkin T (2007) Utilizing and conserving agrobiodiversity in agricultural landscapes. Agr Ecosyst Environ 121:196–210CrossRefGoogle Scholar
  44. Joshi BK, Upadhyay MP, Gauchan D, Sthapit BR, Joshi KD (2004) Red listing of agricultural crop species, varieties and landraces. Nepal Agric Res J 5:73–80Google Scholar
  45. Joshi BK, Ghimire KH, Gauchan D, Singh D, Paudel MN (2017) Agrobiodiversity: conservation strategies, methods and action plans. In: Joshi BK, Gauchan D (ed) Rebuilding local seed system of native crops in earthquake affected areas of Nepal, Proceedings of Sharingshop, Kathmandu. NAGRC, BI and Crop Trust, NepalGoogle Scholar
  46. Keenleyside C, Tucker GM (2010) Farmland abandonment in the EU: an assessment of trends and prospects report prepared for WWF. Institute for European Environmental Policy, LondonGoogle Scholar
  47. Laghetti G, Hammer K, Perrino P (1993) Collecting in north-west Italy. FAO/IBPGR Plant Genet Res Newslett 91:23Google Scholar
  48. Laghetti G, Bisignano V, Urbano M (2018) Genetic resources of vegetable crops and their safeguarding in Italy. Horticult Int J 2:72–74Google Scholar
  49. Last L, Arndorfer M, Balàzs K, Dennis P, Dyman T et al (2014) Indicators for the on-farm assessment of crop cultivar and livestock breed diversity: a survey-based participatory approach. Biodivers Conserv 23:3051–3071CrossRefGoogle Scholar
  50. Law 2015/194 (2015) Disposizioni per la tutela e la valorizzazione della biodiversità di interesse agricolo e alimentare. https://www.gazzettaufficiale.it/eli/gu/2015/12/11/288/sg/pdf. Accessed 01 September 2019Google Scholar
  51. Legislative Decree 2013/9167 (2013) Procedura per la presentazione e l’istruttoria delle domande di iscrizione alla sezione delle varietà da conservazione del registro nazionale delle varietà di specie agrarie e ortive. http://www.galdeiduelaghi.org/assets/Uploads/bandi/323/SEO4215-10-2013.pdf. Accessed 01 September 2019
  52. Liu Z, Pan J (2017) A practical method for extending the biuret assay to protein determination of corn-based products. Food Chem 224:289–293PubMedCrossRefGoogle Scholar
  53. MacDonald-Wicks LK, Wood LG, Garg ML (2006) Methodology for the determination of biological antioxidant capacity in vitro: a review. J Sci Food Agric 86:2046–2056CrossRefGoogle Scholar
  54. Magni C, Scarafoni A, Herndl A et al (2007) Combined 2D electrophoretic approaches for the study of white lupin mature seed storage proteome. Phytochemistry 68:997–1007PubMedCrossRefGoogle Scholar
  55. Malvar RA, Butròn A, Alvarez A, Padilla G, Cartea ME, Revilla P, Ordàs A (2007) Yield performance of the European Union Maize Landrace Core Collection under multiple corn borer infestations. Crop Prot 26:775–781CrossRefGoogle Scholar
  56. Maxted N, Magos Brehm J, Kell S (2013) Resource book for preparation of national conservation plans for crop wild relatives and landraces. University of Birmingham, United KingdomGoogle Scholar
  57. McKie VA, McCleary BV (2016) A novel and rapid colorimetric method for measuring total phosphorus and phytic acid in foods and animal feeds. J AOAC Int 99:738–743CrossRefGoogle Scholar
  58. Meseka S, Menkir A, Obeng-Antwi K (2015) Exploitation of beneficial alleles from maize (Zea mays) landraces to enhance performance of an elite variety in water stress environments. Euphytica 201:149–160CrossRefGoogle Scholar
  59. Montesano V, Negro D, Sarli G, Logozzo G, Spagnoletti Zeuli P (2012) Landraces in Inland areas of the Basilicata region, Italy: monitoring and perspectives for on farm conservation. Genet Resour Crop Evol 59:701–716CrossRefGoogle Scholar
  60. Nazzaro F, Orlando P, Fratianni F, Di Luccia A, Coppola F (2012) Protein analysis-on-chip systems in foodomics. Nutrients 4:1475–1489PubMedPubMedCentralCrossRefGoogle Scholar
  61. Negri V (2003) Landraces in central Italy: where and why they are conserved and perspectives for their on farm conservation. Genet Resour Crop Evol 50:871–885CrossRefGoogle Scholar
  62. Negri V (2005) Agro-biodiversity conservation in Europe: ethical issues. J Agric Environ Ethics 18:3–25CrossRefGoogle Scholar
  63. Negri V, Donnini D, Gigante D, Landucci F, Pacicco L, Panella L, Torricelli R, Venanzoni R (2013) The first Italian inventory of in situ maintained landraces. http://vnr.unipg.it/PGRSecure/html/national_inventory.html. Accessed 11 April 2019
  64. Newton AC, Akar T, Baresel JP et al (2010) Cereal landraces for sustainable agriculture: a review. Agron Sust Dev 30:237–269CrossRefGoogle Scholar
  65. NORDREGIO (2004) Mountain Areas in Europe: Analysis of mountain areas in EU member states, acceding and other European countries. Commissioned report by the European Commission—DG Regional Policy, Brussels. https://ec.europa.eu/regional_policy/sources/docgener/studies/pdf/montagne/mount1.pdf. Accessed 11 April 2019
  66. O’Donnell K, Sharrock S (2017) The contribution of botanic gardens to ex situ conservation through seed banking. Plant Divers 39:373–378PubMedPubMedCentralCrossRefGoogle Scholar
  67. Orsenigo S, Abeli T, Schiavi M, Cauzzi P, Guzzon F, Ardenghi MNG, Rossi G, Vagge I (2018) Morphological characterisation of Cucurbita maxima Duchesne (Cucurbitaceae) landraces from the Po Valley (Northern Italy). Ital J Agron 13:338–342CrossRefGoogle Scholar
  68. Pacicco L, Bodesmo M, Torricelli R, Negri V (2018) A methodological approach to identify agro-biodiversity hotspots for priority in situ conservation of plant genetic resources. PLoS ONE 13:e0197709PubMedPubMedCentralCrossRefGoogle Scholar
  69. Paton AJ, Brummitt N, Govaerts R, Harman K, Hinchcliffe S, Allkin B, Lughadha EN (2008) Target 1 of the global strategy for plant conservation: a working list of all known plant species progress and prospects. Taxon 57:602–611Google Scholar
  70. Peres S (2016) Saving the gene pool for the future: seed banks as archives. Stud Hist Philos Biol 55:96–104CrossRefGoogle Scholar
  71. Pignatti S (1982) Flora d’Italia. Edagricole, BolognaGoogle Scholar
  72. Puglisi D, Landoni M, Cassani E, Toschi I, Lucchini G, Cesari V, Borlini G, Scapin A, Pilu R (2018) Traditional farmers’ varieties: a valuable source of genetic variability for biofortification programs. Maydica 63:1–10Google Scholar
  73. R Development Core Team (2018) R: A language and environment or statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.r-project.org. Accessed 11 April 2019
  74. Rischkowsky B, Pilling D (2007) The State of the World’s animal genetic Resources for food and agriculture—in brief. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
  75. Sharrock S, Oldfield S, Wilson O (2014) Plant Conservation Report 2014: a review of progress in implementation of the Global Strategy for Plant Conservation 2011-2020. CBD Technical Series 81. Secretariat of the Convention on Biological Diversity and Botanic Gardens Conservation International, Montréal-RichmondGoogle Scholar
  76. Singleton VL, Orthofer R, Lamuela-Raventós RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299:152–178CrossRefGoogle Scholar
  77. Spataro G, Negri V (2013) The European seed legislation on conservation varieties: focus, implementation, present and future impact on landrace on farm conservation. Genet Resour Crop Evol 60:2421–2430CrossRefGoogle Scholar
  78. Srinivasan CC, Thirtle C, Palladino P (2003) Winter wheat in England and Wales, 1923–1995: what do indices of genetic diversity reveal? Plant Genet Resour 1:43–57CrossRefGoogle Scholar
  79. Terres JM, Nisini L, Anguiano E. (2013) Assessing the risk of farmland abandonment in the EU. Final report EUR 25783EN, Joint Research Centre of the European Commission, LuxembourgGoogle Scholar
  80. United Nations (2015) Transforming our world: the 2030 Agenda for Sustainable Development. A/RES/70/1. https://undocs.org/A/RES/70/1. Accessed 11 April 2019
  81. USDA (2017) Italy exporter guide 2017. Global Agricultural Information Network, report number: IT1771Google Scholar
  82. Veteläinen M, Negri V, Maxted N (2009) European landraces: on-farm conservation management and use. Biodiversity technical bulletin 15. Biodiversity International, RomeGoogle Scholar
  83. Wood D, Lenné JM (1997) The conservation of agrobiodiversity on-farm: questioning the emerging paradigm. Biodivers Conserv 6:109–129CrossRefGoogle Scholar
  84. Yadav OP, Bidinger FR (2007) Utilization, diversification and improvement of landraces for enhancing pearl millet productivity in arid environments. Ann Arid Zone 46:49–57Google Scholar
  85. Zeven AC (1998) Landraces: a review of definitions and classifications. Euphytica 104:127–139CrossRefGoogle Scholar
  86. Zilić S, Serpen A, Akillioğlu G, Gökmen V, Vančetović J (2012) Phenolic compounds, carotenoids, anthocyanins, and antioxidant capacity of colored maize (Zea mays) kernels. J Agric Food Chem 60:1224–1231PubMedCrossRefPubMedCentralGoogle Scholar

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Authors and Affiliations

  1. 1.Centre of Applied Studies for the Sustainable Management and Protection of Mountain Areas, CRC Ge.S.Di.Mont.University of MilanEdoloItaly
  2. 2.Department of Agricultural and Environmental Sciences-Production, Landscape and AgroenergyUniversity of MilanMilanItaly
  3. 3.Department of Food, Environmental and Nutritional SciencesUniversity of MilanMilanItaly

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