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Biodiversity and Conservation

, Volume 21, Issue 2, pp 343–361 | Cite as

Island endemism, morphological stasis, and possible cryptic speciation in two coral reef, commensal Leucothoid amphipod species throughout Florida and the Caribbean

  • Vincent P. Richards
  • Michael J. Stanhope
  • Mahmood S. Shivji
Original Paper

Abstract

Coral reefs are believed to be one of the most diverse ecosystems, but the true magnitude of their biodiversity and patterns of endemism is uncertain. This uncertainty stems partly from the relative paucity of investigations on the small, difficult to collect taxa (cryptofauna) that may make up the majority of reef biodiversity and require specialized expertise for morphological identification. To assess the extent of diversity in some of the reef micro-cryptofauna, we analyzed 414 bp of the mitochondrial cytochrome oxidase subunit I gene from 556 individuals representing two brooding amphipod species (Leucothoe ashleyae and Leucothoe kensleyi). These amphipods are commensal inside the branching vase sponge Callyspongia vaginalis, and were sampled throughout Florida and the Caribbean. Phylogenetic analyses revealed 11 deeply divergent, strongly supported lineages (seven L. ashleyae and four L. kensleyi) each with very narrow geographic ranges. The level of intraspecific lineage divergence for both morphospecies was among the highest reported for any marine crustacean (12.4–26.0% uncorrected), and exceeded that of congeners from nine diverse amphipod families, as well as the patristic genetic distance suggested as a threshold for crustacean species delineation. These findings suggest a history of cryptic speciation within each morphospecies, concomitant with a pronounced period of morphological stasis involving each of the morphotypes. The observation of multiple, highly divergent, evolutionary significant units, each endemic to Florida and Caribbean island locations, supports the emerging view that coral reef biodiversity, especially in the cryptofaunal component, is likely vastly underestimated.

Keywords

Cryptic species Morphological stasis Coral reef species diversity Leucothoid amphipods 

Notes

Acknowledgments

We thank B. Riegl, S. Purkis, J. Thomas, D. Chapman, R. Hernandez, Caribbean Research and Management of Biodiversity (CARMABI) in Curaçao, Anthony’s Key Resort in Roatan, the Smithsonian research facility at Carrie Bow Cay, the WCS research facility at Glovers Reef for help with sample collections, and M. Debiasse, K. Klebba for lab assistance. This study was supported by a National Oceanic and Atmospheric Administration Coastal Ocean Program (#NA16OA2413) grant to the National Coral Reef Institute and by the Guy Harvey Research Institute. This contribution is NCRI publication number 137.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Vincent P. Richards
    • 1
    • 2
  • Michael J. Stanhope
    • 3
  • Mahmood S. Shivji
    • 1
  1. 1.Oceanographic CenterNational Coral Reef Institute, Nova Southeastern UniversityDania BeachUSA
  2. 2.Department of Population Medicine and Diagnostic SciencesCollege of Veterinary Medicine, Cornell UniversityIthacaUSA
  3. 3.Department of Population Medicine and Diagnostic SciencesCollege of Veterinary Medicine, Cornell UniversityIthacaUSA

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