AFLPs and morphological diversity of Phaseolus lunatus L. in Cuban home gardens: approaches to recovering the lost ex situ collection
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The genetic diversity of 76 accessions of lima bean (Phaseolus lunatus L.), collected mostly from home gardens, was assessed with AFLPs and seed descriptors to evaluate the potential for recovering a lost ex situ collection in Cuba. The sample contained 60 accessions collected from 25 home gardens in the three main geographical regions of Cuba and represented the three cultivated types found on the island. Four more accessions were part of the former ex situ collection and the remaining 12 accessions were selected from the world bean collection held at the International Center for Tropical Agriculture. Some morphological measurements discriminated among cultivated types. The analysis of 62 polymorphic bands obtained with two AFLP primer combinations indicated that the three cultivated bean types were comparable in terms of molecular diversity and that no pattern of variation was associated with geographical distribution. However, a multiple correspondence analysis with the same molecular data detected different genetic groups. Three of these groups included all the cultivated accessions collected from home gardens, but could not be explained by the seed descriptors. The results therefore suggest that a scientifically sound collecting strategy to recover the former Cuban ex situ gene bank should consider combining geographical, morphological, and molecular data. The findings also suggest that any proposed methodologies should be considered before developing a conservation strategy based on an ex situ or combined ex situ and in situ approaches.
KeywordsGenetic structure In situ conservation Lima bean Molecular diversity Multiple correspondence analysis Phaseolus lunatus
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This research was a spin-off from the global project on genetic resources in home gardens carried out by the International Plant Genetic Resources Institute (IPGRI) and entitled Contribution of Home Gardens to the in situ Conservation of Plant Genetic Resources in Farming Systems, which was partially funded by the German Federal Ministry for Economic Cooperation and Development (BMZ) through GTZ (Deutsche Gesellschaft für Technische Zusammenarbeit). The authors also wish to express their appreciation to Maritza García and Fidel Hernández (Estación Ecológica ‘Sierra del Rosario’) and to Celerina Giraudy (Unión de Servicios Ambientales de Guantánamo), members of the Cuban component of the global Project’s research team for their help in collecting samples and morphological characterization; to Joe Tohme (CIAT) for allowing use of laboratory facilities at the CIAT Biotechnology Unit; to Luigi Guarino, Xavier Scheldeman, José Luis Chávez, and V. Ramanatha Rao for their comments that helped improve the manuscript.
- Allard RW (1967) Principios de la mejora genética de las plantas. Edición Revolucionaria, La Habana, CubaGoogle Scholar
- Castiñeiras L, Esquivel M, Rivero N, Mariño A (1991) Variabilidad en semillas de P. lunatus L. colectadas en Cuba. Revista del Jardín Botánico Nacional 12:109–114Google Scholar
- Castiñeiras L, Shagarodsky T, Fuentes V, Fundora Z, Fernández L, Moreno V, Barrios O, Sánchez P, Walón L, Pérez MF, Puldón G (2001) El frijol caballero (Phaseolus lunatus L.) un cultivo marginal y en peligro de erosión genética en Cuba. Revista del Jardín Botánico Nacional 22(1):133–138Google Scholar
- Castiñeiras L, Fundora-Mayor Z, Shagarodsky T, Moreno V, Barrios O, Fernández L, Cristóbal R (2002) Contribution of home gardens to in situ conservation of plant genetic resources—Cuban component. In: Watson JW, Eyzaguirre PB (eds) Proceedings of the second international home gardens workshop: contribution of home gardens to in situ conservation of plant genetic resources in farming systems. Witzenhausen, Federal Republic of Germany, 17–19 July 2001, IPGRI, Rome Italy, pp 42–56Google Scholar
- de Vicente MC (2002) Molecular techniques to facilitate prioritization of plant genetic resources conservation and further research. AgBiotechNet 4(ABN092):1–5Google Scholar
- Fundora Z, Shagarodsky T, Castiñeiras L (2004) Sampling methods for genetic diversity study in home gardens in Cuba. In: Eyzaguirre P, Linares O (eds) Home gardens and agrobiodiversiy. Smithsonian Books, Washington, pp 56–57Google Scholar
- Hoogendijk M, Williams DE (2002) Characterizing the genetic diversity of home gardens crops: some examples from the Americas. In: Watson JW, Eyzaguirre PB (eds), Proceedings of the second international home gardens workshop: contribution of home gardens to in situ conservation of plant genetic resources in farming systems. Witzenhausen, Federal Republic of Germany, 17–19 July 2001, IPGRI, Rome, Italy, pp. 34–40Google Scholar
- IBPGR (1982) Descriptors of Phaseolus lunatus. International Board for Plant Genetic Resources, Rome, ItalyGoogle Scholar
- Lioi L, Esquivel M, Castiñeiras L, Hammer K (1991) Lima bean (Phaseolus lunatus L.) landraces from Cuba: electrophoresis analysis of seed storage proteins. Biol Zent bl 110:76–79Google Scholar
- Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York, USAGoogle Scholar
- Shagarodsky T, Castiñeiras L, Fuentes V, Cristóbal R (2004) Characterization in situ of the variability of sapote or mamey in Cuban home gardens. In: Eyzaguirre P, Linares O (eds) Home gardens and agrobiodiversity. Smithsonian Books, Washington, pp 266–281Google Scholar
- Watson JW, Eyzaguirre PB (eds) (2002) Proceedings of the second international home gardens workshop: contribution of home gardens to in situ conservation of plant genetic resources in farming systems, 17–19 July 2001, Witzenhausen, Federal Republic of Germany. International Plant Genetic Resources Institute, RomeGoogle Scholar