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Biodiversity and Conservation

, Volume 16, Issue 10, pp 2847–2865 | Cite as

AFLPs and morphological diversity of Phaseolus lunatus L. in Cuban home gardens: approaches to recovering the lost ex situ collection

  • Leonor Castiñeiras
  • Félix Alberto Guzmán
  • Myriam Cristina Duque
  • Tomás Shagarodsky
  • Raúl Cristóbal
  • M. Carmen de VicenteEmail author
Original Paper

Abstract

The genetic diversity of 76 accessions of lima bean (Phaseolus lunatus L.), collected mostly from home gardens, was assessed with AFLPs and seed descriptors to evaluate the potential for recovering a lost ex situ collection in Cuba. The sample contained 60 accessions collected from 25 home gardens in the three main geographical regions of Cuba and represented the three cultivated types found on the island. Four more accessions were part of the former ex situ collection and the remaining 12 accessions were selected from the world bean collection held at the International Center for Tropical Agriculture. Some morphological measurements discriminated among cultivated types. The analysis of 62 polymorphic bands obtained with two AFLP primer combinations indicated that the three cultivated bean types were comparable in terms of molecular diversity and that no pattern of variation was associated with geographical distribution. However, a multiple correspondence analysis with the same molecular data detected different genetic groups. Three of these groups included all the cultivated accessions collected from home gardens, but could not be explained by the seed descriptors. The results therefore suggest that a scientifically sound collecting strategy to recover the former Cuban ex situ gene bank should consider combining geographical, morphological, and molecular data. The findings also suggest that any proposed methodologies should be considered before developing a conservation strategy based on an ex situ or combined ex situ and in situ approaches.

Keywords

Genetic structure In situ conservation Lima bean Molecular diversity Multiple correspondence analysis Phaseolus lunatus 

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Notes

Acknowledgements

This research was a spin-off from the global project on genetic resources in home gardens carried out by the International Plant Genetic Resources Institute (IPGRI) and entitled Contribution of Home Gardens to the in situ Conservation of Plant Genetic Resources in Farming Systems, which was partially funded by the German Federal Ministry for Economic Cooperation and Development (BMZ) through GTZ (Deutsche Gesellschaft für Technische Zusammenarbeit). The authors also wish to express their appreciation to Maritza García and Fidel Hernández (Estación Ecológica ‘Sierra del Rosario’) and to Celerina Giraudy (Unión de Servicios Ambientales de Guantánamo), members of the Cuban component of the global Project’s research team for their help in collecting samples and morphological characterization; to Joe Tohme (CIAT) for allowing use of laboratory facilities at the CIAT Biotechnology Unit; to Luigi Guarino, Xavier Scheldeman, José Luis Chávez, and V. Ramanatha Rao for their comments that helped improve the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Leonor Castiñeiras
    • 1
  • Félix Alberto Guzmán
    • 2
  • Myriam Cristina Duque
    • 3
  • Tomás Shagarodsky
    • 1
  • Raúl Cristóbal
    • 1
  • M. Carmen de Vicente
    • 2
    Email author
  1. 1.Instituto de Investigaciones Fundamentales en Agricultura Tropical Alejandro de Humboldt (INIFAT)Santiago de las Vegas, Ciudad de la HabanaCuba
  2. 2.Regional Office for the Americas International Plant Genetic Resources Institute (IPGRI)c/o CIATCaliColombia
  3. 3.Centro Internacional de Agricultura Tropical (CIAT)CaliColombia

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