Biodiversity & Conservation

, Volume 15, Issue 14, pp 4357–4373 | Cite as

Genetic Variation in Three Endangered Species of Encholirium (Bromeliaceae) from Cadeia do Espinhaço, Brazil, Setected using RAPD Markers

  • Marcelo Mattos CavallariEmail author
  • Rafaela Campostrini Forzza
  • Elizabeth Ann Veasey
  • Maria Imaculada Zucchi
  • Giancarlo Conde Xavier Oliveira


Encholirium is a Brazilian genus of Bromeliaceae that occurs exclusively in rocky landscapes. This work aimed to generate basic information for the conservation of three Encholirium species that are endemic to the rocky mountains of Cadeia do Espinhaço, employing population genetic analyses. E. pedicellatum and E. biflorum have only one very small population each, both occurring in unprotected, private land sites, being critically endangered. E. subsecundum is more widespread, and some of its populations dwell in protected areas. Five Random Amplified Polymorphic DNA (RAPD) primers generated approximately 60 polymorphic bands for each species. This technique demonstrated the presence of a single RAPD profile for every individual sampled (except for one clone found in E. biflorum). High levels of genetic variability were not expected, due to the clonal habit of the plants and small size of the populations. Populations of E. biflorum and E. pedicellatum presented, respectively, 16.06% (Φ st  = 0.16, p<0.001) and 8.44% (Φ st  = 0.08, p<0.001) of the total genetic diversity attributable to genetic differences among groups within the populations. In E. subsecundum, 14.52% (Φ st  = 0.15, p<0.001) of the total genetic diversity was found among populations. Estimates of the Shannon’s Diversity Index provided similar results. These results are valuable for the development of conservation strategies.


Bromeliaceae Conservation biology Encholirium Population genetic structure RAPD Rare plants 


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  1. Allnutt T.R., Newton A.C., Premoli A. and Lara A. (2003). Genetic variation in the threatened South American conifer Pilgerodendron uviferum (Cupressaceae), detected using RAPD markers. Biol. Conserv. 114: 245–253CrossRefGoogle Scholar
  2. Ayres D.R. and Ryan F.J. (1999). Genetic diversity and structure of the narrow endemic Wyethia reticulata its congener W. bolanderi (Asteraceae) using RAPD and allozyme techniques. Am. J. Bot. 86: 344–353PubMedCrossRefGoogle Scholar
  3. Bekessy S.A., Allnutt T.R., Premoli A.C., Lara A., Ennos R.A., Burgman M.A., Cortes M. and Newton A.C. (2002). Genetic variation in the vulnerable and endemic Monkey Puzzle treedetected using RAPDs. Heredity 88: 243–249PubMedCrossRefGoogle Scholar
  4. Chung M.G., Chung J.M. and Epperson B.K. (1999). Spatial genetic structure of allozyme polymorphisms within populations of Rhus trichocarpa (Anacardiaceae). Silvae Genetica 48: 223–227Google Scholar
  5. Coelho A.S.G. 2000. Dboot: avaliação dos erros associados a estimativas de distâncias/ similaridades genéticas através do procedimento de bootstrap com número variável de marcadores (software).[Dboot: Assessment of Errors Associated to Estimates of Genetic Distances/Similarities by Bootstrapping with a Variable Number of Markers (software)]. Laboratório de Genética Vegetal, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Brazil.Google Scholar
  6. Coelho A.S.G. 2003. Bood-P: avaliação de dendrogramas baseados em estimativas de distâncias/ similaridades genéticas através do procedimento de bootstrap (software). [Bood-P: Assessment of Dendrograms based on Estimates of Genetic Distances/Similarities by Bootstrapping (software)]. Laboratório de Genética Vegetal, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia.Google Scholar
  7. Costa C.M.R., Hermann G., Martins C.S., Lins L.V. and Lamas I.R. (1998). Biodiversidade em Minas Gerais: um Atlas para sua conservação. Fundação Biodiversitas, Belo HorizonteGoogle Scholar
  8. Doyle J.J. and Doyle J.L. (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13–15Google Scholar
  9. Ellstrand N.C. and Elam D.R. (1993). Population genetic consequences of small population size: implications for plant conservation. Annu. Rev. Ecol. Syst. 24: 217–242CrossRefGoogle Scholar
  10. Esselman E.J., Jianqiang L., Crawford D.J., Winduss J.L. and Wolfe A.D. (1999). Clonal diversity in the rare Calamagrostis porteri spp insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Mol. Ecol. 8: 443–451CrossRefGoogle Scholar
  11. Excoffier L., Smouse P.E. and Quattro J.M. (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes – application to human mitochondrial – DNA restriction data. Genetics 131: 479–491PubMedGoogle Scholar
  12. Fischer M., Husi R., Prati D., Peintinger M., van Kleunen M. and Schmid B. (2000). RAPD variation among and within small and large populations of the rare clonal plant Ranunculus reptans (Ranunculaceae). Am. J. Bot. 87: 1128–1137PubMedCrossRefGoogle Scholar
  13. Forzza R.C. (2005). Revisão taxonômica de Encholirium Mart. ex. Schult. & Schult. f. (Pitcairnioideae – Bromeliaceae). Boletim de Botânica da Universidade de São Paulo 23: 1–60Google Scholar
  14. Forzza R.C., Christianini A.V. and Wanderley M.G.L. (2003). Encholirium (Pitcairnioideae – Bromeliaceae): conhecimento atual e sugestões para conservação. Vidalia 1: 7–20Google Scholar
  15. Gentry A.H. (1986). Endemism in tropical versus temperate plant communities. In: Soulé, M.E. (eds) Conservation Biology – The Science of Scarcity and Diversity, Sinauer Associates, Sunderland Massachusetts 153–181Google Scholar
  16. Giulietti A.M., Pirani J.R. and Harley R.M. (1997). Espinhaço range region. In: Davis, S.D., Heywood, V.H., Herrera-Macbryde, O., Villa-Lobos, J., and Hamilton, A. (eds) Centres of Plant Diversity: A Guide and Strategy for Their Conservation. Volume 3: The Americas, IUCN Publications Unit, Cambridge 397–404Google Scholar
  17. Hamrick J.L. and Godt J.W. (1989). Allozyme diversity in plant species. In: Brown, A.H.D., Clegg, M.T. and Kahler, A.L. (eds) Plant Population Genetics, Breeding, and Genetic Resources, Sinauer Associates, Sunderland, Massachusetts 43–63Google Scholar
  18. Holsinger K.E. and Gottlieb L.D. (1991). Conservation of rare and endangered plants: principles and prospects. In: Falk, D.A. and Holsinger, K.E. (eds) Genetics and Conservation of Rare Plants, Oxford, Oxford University Press 195–223Google Scholar
  19. Huff D.R., Peakall R. and Smouse P.E. (1993). RAPD variation within and among natural populations of outcrossing buffalograss [Buchloe dactyloides (Nutt.) Engelm.]. Theor. Appl. Genet. 86: 927–934CrossRefGoogle Scholar
  20. James E.A. and Ashburner G.R. (1997). Intraspecific variation in Astelia australiana (Liliaceae) and implications for the conservation of this Australian species. Biol. Conserv. 82: 253–261CrossRefGoogle Scholar
  21. Kreher S.A., Foré S.A. and Collins B.S. (2000). Genetic variation within and among patches of the clonal species Vaccinium stamineum L. Mol. Ecol. 9: 1247–1252PubMedCrossRefGoogle Scholar
  22. Lacerda D.R., Acedo M.D.P., Lemos-Filho J.P. and Lovato M.B. (2001). Genetic diversity and structure of natural populations of Plathymenia reticulata (Mimosoideae), a tropical tree from Brazilian Cerrado. Mol. Ecol. 10: 1143–1152PubMedCrossRefGoogle Scholar
  23. Lewontin R.C. (1972). The Apportionment of Human Diversity. Evol. Biol. 6: 381–494Google Scholar
  24. Maki M. and Horie S. (1999). Random amplified polymorphic DNA (RAPD) markers reveal less genetic variation in the endangered plant Cerastium fischerianum var. molle than in the widespread conspecific C. fischerianum var. fischerianum (Caryophyllaceae). Mol. Ecol. 8: 145–150CrossRefGoogle Scholar
  25. Persson H.A. and Gustavsson B.A. (2001). The extent of clonality and genetic diversity in lingonberry (Vaccinium vitis- idaea L.) revealed by RAPDs and leaf-shape analysis. Mol. Ecol. 10: 1385–1397PubMedCrossRefGoogle Scholar
  26. Rohlf F.J. (1989). NTSYS-Pc: Numerical Taxonomy and Multivariate Analysis System. Exerter Publisher, New York, 210Google Scholar
  27. Sazima I., Vogel S. and Sazima M. (1989). Bat pollination of Encholirium glazioviia terrestrial bromeliad. Plant Syst. Evol. 168: 167–179CrossRefGoogle Scholar
  28. Schneider S., Roessli D. and Excoffier L. (2000). Arlequin ver. 2000: A Software for Population Data Analysis (software). Genetic and Biometry Laboratory, University of Geneva, GenevaGoogle Scholar
  29. Tansley S.A. and Brown C.R. (2000). RAPD variation in the rare and endangered Leucadendron elimense (Proteaceae): implications for their conservation. Biol. Conserv. 95: 39–48CrossRefGoogle Scholar
  30. Welsh J. and McClelland M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 303–306Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Marcelo Mattos Cavallari
    • 1
    Email author
  • Rafaela Campostrini Forzza
    • 2
  • Elizabeth Ann Veasey
    • 1
  • Maria Imaculada Zucchi
    • 1
  • Giancarlo Conde Xavier Oliveira
    • 1
  1. 1.Departamento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz’ (ESALQ)Universidade de São PauloPiracicabaBrazil
  2. 2.Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)Rio de JaneiroBrazil

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