Skip to main content

Advertisement

Log in

Recent confirmation of a single haplotype in the Italian population of Vespa velutina

  • Invasion Note
  • Published:
Biological Invasions Aims and scope Submit manuscript

Abstract

Vespa velutina Lepeletier, 1836 (Hymenoptera: Vespidae), the yellow-legged hornet, is a social wasp native to North of India, China and tropical and subtropical areas of Southeast Asia. Adults are predators of a wide variety of arthropods, including honey bees. Vespa velutina has been unintentionally introduced in southwestern France before 2004 and its widely spreading across the country was followed by an impressive colonization of several areas within mainland Europe and overseas. In Italy, V. velutina has been officially reported in June 2013 in Imperia province, Liguria region (northern Italy) and is now well established in the western part of this region and occasionally reported in Piedmont region. More recently, it has been spotted in Veneto, Lombardy and Tuscany regions. In order to investigate the phylogenetic relationships and the origin of Italian V. velutina nigrithorax specimens, we performed a phylogenetic analysis of thirty-three samples, collected in 2012, 2016, 2017 and 2018 from different sites (Veneto, Piedmont and Liguria regions) based on mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequence analysis. The phylogenetic analysis as well the haplotypes study confirmed the placement of 33 Italian specimens within a cluster containing the other European samples plus Chinese specimens collected in Jiangsu and Zhejiang. Our results strongly supported the view that the Italian specimens of V. velutina nigrithorax derived from the spreading southward of the V. velutina nigrithorax population initially established in France, which is also moving northward (Belgium, The Netherlands, Germany, United Kingdom).

Graphic abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  • Arca M (2012) Caractérisation génétique et étude comportementale d’une espèce envahissante en France: Vespa velutina Lepeletier (Hymenoptera, Vespidae). In: Ph.D. dissertation. Université Pierre et Marie Curie, Paris

  • Arca M, Papachristoforou A, Mougel F, Rortais A, Monceau K, Bonnard O, Tardy P, Thiéry D, Silvain JF, Arnold G (2014) Defensive behaviour of Apis mellifera against Vespa velutina in France: testing whether European honeybees can develop an effective collective defence against a new predator. Behav Proc 106:122–129. https://doi.org/10.1016/j.beproc.2014.05.002

    Article  Google Scholar 

  • Arca M, Mougel F, Guillemaud T, Dupas S, Rome Q, Perrard A, Muller F, Fossund A, Capdevielledula C, Torres-Leguizamon M, Chen XX, Tan JL, Jung C, Villemant C, Arnold G, Silvain JF (2015) Reconstructing the invasion and the demographic history of the yellow-legged hornet, Vespa velutina, in Europe. Biol Invasions 17:2357–2371. https://doi.org/10.1007/s10530-015-0880-9

    Article  Google Scholar 

  • Bandelt HJ, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:37–48

    Article  CAS  Google Scholar 

  • Bertolino S, Lioy S, Laurino D, Manino A, Porporato M (2016) Spread of the invasive yellow-legged hornet Vespa velutina (Hymenoptera: Vespidae) in Italy. Appl Entomol Zool 51(4):589–597. https://doi.org/10.1007/s13355-016-0435-2

    Article  Google Scholar 

  • Budge GE, Hodgetts J, Jones EP, Ostojá-Starzewski JC, Hall J, Tomkies JV, Semmence N, Brown M, Wakefield M, Stainton K (2017) The invasion, provenance and diversity of Vespa velutina Lepeletier (Hymenoptera: Vespidae) in Great Britain. PLoS One 12(9):e0185172. https://doi.org/10.1371/journal.pone.0185172

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Carpenter JM, Kojima J (1997) Checklist of the Species in the Subfamily Vespinae (Insecta: Hymenoptera: Vespidae). Nat Hist Bull Ibaraki Univ 1:51–92

    Google Scholar 

  • Demichelis S, Manino A, Porporato M (2013) Trovato il primo nido di Vespa velutina a Vallecrosia (IM). In: Comunicato Stampa. Università Degli Studi di Torino, Turin. https://www.vespavelutina.eu/Portals/0/Users/152/52/152/2013.10.03%20Comunicato%20Stampa%20-%20La%20Vespa%20velutina%20%C3%A8%20arrivata%20in%20Piemonte.pdf?ver=2016-08-22-143632-943. Accessed 5 July 2019

  • Demichelis S, Manino A, Minuto G, Mariotti M, Porporato M (2014) Social wasp trapping in north west Italy: Comparison of different bait-traps and first detection of Vespa velutina. Bull Insectol 67:307–317

    Google Scholar 

  • EU (2016) Commission Implementing Regulation (EU) 2016/1141 of 13 July 2016 adopting a list of invasive alien species of Union concern pursuant to Regulation (EU) No. 1143/2014 of the European Parliament and of the Council. OJ L 189:14.7.2016, pp 4–8

  • Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3(5):294–299

    CAS  Google Scholar 

  • Haxaire J, Bouguet JP, Tamisier JP (2006) Vespa velutina Lepeletier, 1836, une redoutable nouveauté pour la faune de France (Hym., Vespidae). Bull Soc Entomol Fr 111:194

  • Hoang DT, Chernomor O, von Haeseler A, Minh BQ, Vinh LS (2018) UFBoot2: improving the ultrafast bootstrap approximation. Mol Biol Evol 35:518–522. https://doi.org/10.1093/molbev/msx281

    Article  CAS  PubMed  Google Scholar 

  • Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nat Methods 14:587–589. https://doi.org/10.1038/nmeth.4285

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ken T, Hepburn HR, Radloff SE, Yusheng Y, Yiqiu L, Danyin Z, Neumann P (2005) Heat-balling wasps by honeybees. Naturwissenschaften 92:492–495. https://doi.org/10.1007/s00114-005-0026-5

    Article  CAS  Google Scholar 

  • Matsuura M, Yamane S (1990) Biology of vespine wasps. Springer, Berlin

    Book  Google Scholar 

  • Monceau K, Thiery D (2016) Vespa velutina: current situation and perspectives. Atti Acc Naz Ital Entomol LXIV:137–142

    Google Scholar 

  • Monceau K, Arca M, Leprêtre L, Florence M, Bonnard O, Silvain J-F, Maher N, Thiéry D (2013) Native prey and invasive predator patterns of foraging activity: the case of the yellow-legged hornet predation at European honeybee hives. PLoS One 8:1–9. https://doi.org/10.1371/journal.pone.0066492

    Article  CAS  Google Scholar 

  • Monceau K, Bonnard O, Moreau J, Thiéry D (2014a) Spatial distribution of Vespa velutina individuals hunting at domestic honeybee hives: heterogeneity at a local scale. Insect Sci 21:765–774. https://doi.org/10.1111/1744-7917.12090

    Article  Google Scholar 

  • Monceau K, Bonnard O, Thiéry D (2014b) Vespa velutina: a new invasive predator of honeybees in Europe. J Pest Sci 87:1–16. https://doi.org/10.1007/s10340-013-0537-3

    Article  Google Scholar 

  • Nguyen L-T, Schmidt HA, von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Mol Biol Evol 32:268–274. https://doi.org/10.1093/molbev/msu300

    Article  CAS  PubMed  Google Scholar 

  • Pagliano G, Negrisolo E (2005) Hymenoptera Sphecidae. Calderini, p 559

  • Perrard A, Haxaire J, Rortais A, Villemant C (2009) Observations on the colony activity of the Asian hornet Vespa velutina Lepeletier 1836 (Hymenoptera: Vespidae: Vespinae) in France. Ann Soc Entomol Fr 45:119–127

    Article  Google Scholar 

  • Perrard A, Arca M, Rome Q, Muller F, Tan J, Bista S, Nugroho H, Baudoin R, Baylac M, Silvain JF, Carpenter JM, Villemant C (2014) Geographic variation of melanisation patterns in a hornet species: genetic differences, climatic pressures or aposematic constraints? PLoS One 9(4):e94162. https://doi.org/10.1371/journal.pone.0094162

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Quaranta M, Sommaruga A, Balzarini P, Felicioli A (2014) A new species for the bee fauna of Italy: Megachile sculpturalis continues its colonization of Europe. Bull Insectol 67(2):287–293

    Google Scholar 

  • Requier F, Rome Q, Chiron G, Decante D, Marion S, Menard M, Muller F, Villemant C, Henry M (2019) Predation of the invasive Asian hornet affects foraging activity and survival probability of honey bees in Western Europe. J Pest Sci 92(2):567–578. https://doi.org/10.1007/s10340-018-1063-0

    Article  Google Scholar 

  • Rome Q, Muller F, Théry T, Andrivot J, Haubois S, Rosenstiehl E, Villemant C (2011) Monitoring and control modalities of a honeybee predator, the Yellow-legged hornet Vespa velutina nigrithorax (Hymenoptera: Vespidae). Newsletter of the IUCN/SSC Invasive Species Specialist Group. Aliens 31:7–15

    Google Scholar 

  • Takahashi J, Okuyama H, Kiyoshi T, Takeuchi T, Martin SJ (2018) Origins of Vespa velutina hornets that recently invaded Iki Island, Japan and Jersey Island, UK. Mitochondrial DNA Part A, https://doi.org/10.1080/24701394.2018.1538366

  • Takeuchi T, Takahashi R, Kiyoshi T, Nakamura M, Minoshima YN, Takahashi J (2017) The origin and genetic diversity of the yellow-legged hornet, Vespa velutina introduced in Japan. Insect Soc 64:313–320. https://doi.org/10.1080/23802359.2018.1437823

    Article  Google Scholar 

  • Tan K, Radloff SE, Li JJ, Hepburn HR, Yang MX, Zhang LJ, Neumann P (2007) Bee-hawking by the wasp, Vespa velutina, on the honeybees Apis cerana and A. mellifera. Naturwissenschaften 94:469–472. https://doi.org/10.1007/s00114-006-0210-2

    Article  CAS  Google Scholar 

  • Villemant C, Barbet-Massin M, Perrard A, Muller F, Gargominy O, Jiguet F, Rome Q (2011a) Predicting the invasion risk by the alien bee-hawking Yellow-legged hornet Vespa velutina nigrithorax across Europe and other continents with niche models. Biol Conserv 44(9):2142–2150. https://doi.org/10.1016/j.biocon.2011.04.009

    Article  Google Scholar 

  • Villemant C, Muller F, Haubois S, Perrard A, Darrouzet E, Rome Q (2011b) Bilan des travaux (MNHN et IRBI) sur l’invasion en France de Vespa velutina, le frelon asiatique prédateur d’abeilles. In: Barbançon J-M, L’Hostis M (eds) Proceedings of the Journée Scientifique Apicole, Arles, ONIRISFNOSAD, Nantes, 11 February 2011, pp 3–12

Download references

Funding

The work was carried out in the framework of Commission Implementing Regulation (EU) No. 1308/2013 of the European Parliament and of the Council and project ALIEM (Action pour Limiter les risques de diffusion des espèces Introduites Envahissantes en Méditerranée), partially funded by the ‘Programma Interreg Italia-Francia Marittimo 2014–2020. La cooperazione al cuore del Mediterraneo’, axe II.

Author information

Authors and Affiliations

Authors

Contributions

FM conceived the study; FM, FC and LB provided Vespa velutina samples; AG designed the experiments; JB, LZ and AG performed the experiments and analysed the data; EN performed phylogenetic analyses and analysed the data; FM, AG and EN wrote the original draft and all authors reviewed draft of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Anna Granato.

Ethics declarations

Conflict of interest

The authors declare that they have no potential conflict of interest in relation to the study in this paper.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 213 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Granato, A., Negrisolo, E., Bonomi, J. et al. Recent confirmation of a single haplotype in the Italian population of Vespa velutina. Biol Invasions 21, 2811–2817 (2019). https://doi.org/10.1007/s10530-019-02051-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10530-019-02051-4

Keywords

Navigation