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Biological Invasions

, Volume 18, Issue 8, pp 2137–2151 | Cite as

Persistence, dispersal and genetic evolution of recently formed Spartina homoploid hybrids and allopolyploids in Southern England

  • Dalibor Huska
  • Ilia J. Leitch
  • Julie Ferreira de Carvalho
  • Andrew R. Leitch
  • Armel Salmon
  • Malika Ainouche
  • Ales KovarikEmail author
Invasive spartina

Abstract

In Southampton Water, UK, the recent (c. 150 years ago) interspecific hybridisation between Spartina alterniflora (2n = 6x = 62; A-genome) and S. maritima (2n = 6x = 60; M-genome) gave rise to the homoploid hybrid (S. × townsendii, 2n = 6x = 62), and subsequently to the invasive allododecaploid species S. anglica (2n = 12x = 120–124) that has since spread worldwide. To address the question of dynamics of mixed ploidy populations involving these plants, we analysed several Spartina populations (fifty one individuals) in Southern England, UK, one of which was the presumed place of origin of the homoploid hybrid (Hythe). Using a combination of flow cytometry and ribosomal DNA (rDNA) genotyping we were able to identify the genomic composition and ploidy level of each individual analysed. The data show that the homoploid hybrid still dominates the population at Hythe (82 % of individuals collected in that locality) since its origin in the nineteenth century. We also identified S. × townsendii for the first time on Hayling Island (66 % individuals), indicating dispersal beyond its likely origin. The fertile allododecaploid S. anglica was mainly found in populations outside the initial hybridisation site, on Hayling Island and at Eling Marchwood. Quantification of the rDNA contributions from each parental genome showed that the ratios were mostly balanced in S. × townsendii. However, two (3 %) S. anglica individuals analysed have lost nearly all M-genome homeologs, indicating extensive repeat loss. Such variation indicates that despite the presumed single allopolyploid origin of S. anglica and genetic uniformity at other loci, it has undergone substantial changes at the rDNA loci following genome duplication.

Keywords

Spartina Allopolyploidy Cytotypes Genetic markers rDNA Biodiversity 

Notes

Acknowledgments

We thank the New Forest District Council, Associated British Ports, and Northney Marina for granting us permission to collect Spartina plants. We thank Dr Sonia Garcia (University of Barcelona for helpful discussion in the course of manuscript preparation. The work was funded by the Czech Science Foundation (P501/13/10057S), the French CNRS—University of Rennes 1 International Associated Laboratory Ecological Genomics of Polyploidy (ECOGEN), the Partner University Funds, NERC (UK), and the Czech-French research Barrande program.

Supplementary material

10530_2015_956_MOESM1_ESM.pdf (1.6 mb)
Supplementary material 1 (PDF 1637 kb)

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Dalibor Huska
    • 1
  • Ilia J. Leitch
    • 2
  • Julie Ferreira de Carvalho
    • 3
  • Andrew R. Leitch
    • 4
  • Armel Salmon
    • 3
  • Malika Ainouche
    • 3
  • Ales Kovarik
    • 1
    Email author
  1. 1.Institute of BiophysicsCzech Academy of Sciences, v.v.i.BrnoCzech Republic
  2. 2.Jodrell LaboratoryRoyal Botanic Gardens, KewRichmondUK
  3. 3.UMR 6553 ECobio Université de Rennes 1RennesFrance
  4. 4.School of Biological and Chemical SciencesQueen Mary University of LondonLondonUK

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