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Different thermal tolerance and hsp gene expression in invasive and indigenous sibling species of Bemisia tabaci

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Abstract

Temperature is a primary factor that determines geographic insect boundaries and population developments. Insect responses to temperature stress are particularly important in invasive species. Bemisia tabaci is a genetic complex of closely related whiteflies. Invasive and indigenous species were used to test genotype responses to temperature stress. To determine whether thermal tolerance is related to differential expression of heat shock proteins (hsp) under temperature stress in two invasive and native sibling species, commonly referred to as the biotype B and biotype Q of B. tabaci, we analyzed the expression profiles of hsp90 and hsp70 and hsp20 across temperature gradients using real-time quantitative polymerase chain reaction. The B. tabaci invasive species Q is more tolerant to short temperature stress than the indigenous species ZHJ1, and the onset (Ton) or maximal (Tmax) temperature induction of hsp expression in the B. tabaci invasive species Q was generally 2–4°C higher than that found in the indigenous species ZHJ1. Our results demonstrate differences in gene expression between these closely related and sympatric B. tabaci sibling species, suggesting that these differences account for the better adaptation of invasive species over indigenous species. These differences may eventually lead to changes in the local and global distribution of both species in a warmer world.

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Acknowledgments

We appreciate the helpful comments provided by the anonymous reviewers. This work was supported by the National Basic Research and Development Program (Grant No. 2009CB119200) and National Natural Science Foundation of China (No.30800722), Ministry of Science and Technology, China.

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Authors and Affiliations

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, #12, Zhong-Guan-Cun Nan-Da-Jie, Haidian, Beijing, 100081, China

    Hao Yu, Fang Hao Wan & Jan Ying Guo

  2. Department of Entomology, Henan Institute of Science and Technology, Xinxiang, Henan Province, China

    Hao Yu

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  1. Hao Yu
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  2. Fang Hao Wan
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  3. Jan Ying Guo
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Correspondence to Fang Hao Wan.

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Yu, H., Wan, F.H. & Guo, J.Y. Different thermal tolerance and hsp gene expression in invasive and indigenous sibling species of Bemisia tabaci . Biol Invasions 14, 1587–1595 (2012). https://doi.org/10.1007/s10530-012-0171-7

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  • DOI: https://doi.org/10.1007/s10530-012-0171-7

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