Abstract
The virile crayfish (Orconectes virilis) represents a cryptic species complex with several lineages known in the USA, and a wide introduced range. In Europe, O. virilis is an emerging invader, established during the last decade in at least two areas—one in the United Kingdom and another in the Netherlands. We assessed the position of both known European populations within the species complex by sequencing part of the mitochondrial gene for cytochrome c oxidase subunit I. Tested UK and Dutch individuals did not belong to any mitochondrial lineage recorded in North America so far but formed a separate clade, the original distribution area of which is unknown. Additionally sequenced virile crayfish from Iowa (USA) also represented a new clade, suggesting that undiscovered lineage variation within O. virilis remains high. This exemplifies that genetic analyses of invading populations may provide new insights into diversity of a taxon in its original range.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahern D, England J, Ellis A (2008) The virile crayfish, Orconectes virilis (Hagen, 1870) (Crustacea: Decapoda: Cambaridae), identified in the UK. Aquat Inv 3:102–104. doi:10.3391/ai.2008.3.1.18
Arrignon JCV, Gérard P, Krier A et al (1999) The situation in Belgium, France, and Luxembourg. In: Gherardi F, Holdich DM (eds) Crayfish in Europe as alien species. How to make the best of a bad situation? AA Balkema, Rotterdam, Brookfield, pp 129–140
Chucholl C, Daudey T (2008) First record of Orconectes juvenilis (Hagen, 1870) in eastern France: update to the species identity of a recently introduced orconectid crayfish (Crustacea: Astacida). Aquat Inv 3:105–107. doi:10.3391/ai.2008.3.1.19
Costa FO, deWaard JR, Boutillier J et al (2007) Biological identifications through DNA barcodes: the case of the Crustacea. Can J Fish Aquat Sci 64:272–295. doi:10.1139/F07-008
Fetzner JW, Sheehan RJ, Seeb LW (1997) Genetic implications of broodstock selection for crayfish aquaculture in the Midwestern United States. Aquaculture 154:39–55. doi:10.1016/S0044-8486(97)00026-4
Folmer O, Black M, Hoeh W et al (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3:294–299
Hamr P (2002) Orconectes. In: Holdich DM (ed) Biology of freshwater crayfish. Blackwell Science, Oxford, pp 585–608
Hasegawa M, Kishino K, Yano T (1985) Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol 22:160–174. doi:10.1007/BF02101694
Hebert PDN, Cywinska A, Ball SL et al (2003) Biological identifications through DNA barcodes. Proc R Soc Lond B Biol Sci 270:313–321. doi:10.1098/rspb.2002.2218
Henttonen P, Huner JV (1999) The introduction of alien species of crayfish in Europe: a historical introduction. In: Gherardi F, Holdich DM (eds) Crayfish in Europe as alien species. How to make the best of a bad situation? AA Balkema, Rotterdam, Brookfield, pp 13–22
Holdich DM (1999) The negative effects of established crayfish introductions. In: Gherardi F, Holdich DM (eds) Crayfish in Europe as alien species. How to make the best of a bad situation? AA Balkema, Rotterdam, Brookfield, pp 31–47
Holdich DM, Pöckl M (2007) Invasive crustaceans in European inland waters. In: Gherardi F (ed) Freshwater bioinvaders: profiles, distribution, and threats. Springer, Dordrecht, pp 29–75
Hobbs HH Jr (1989) An illustrated checklist of the American crayfishes (Decapoda: Astacidae, Cambaridae and Parastacidae). Smiths Contrib Zool No. 480, pp 236
Lodge DM, Taylor CA, Holdich DM et al (2000) Nonindigenous crayfishes threaten North American freshwater biodiversity: lessons from Europe. Fisheries 25:7–20. doi:10.1577/1548-8446(2000)025<0007:NCTNAF>2.0.CO;2
Mathews LM, Warren AH (2008) A new crayfish of the genus Orconectes Cope, 1872 from southern New England (Crustacea: Decapoda: Cambariidae). Proc Biol Soc Wash 121:374–381. doi:10.2988/08-02.1
Mathews LM, Adams L, Anderson E et al (2008) Genetic and morphological evidence for substantial hidden biodiversity in a freshwater crayfish species complex. Mol Phylogenet Evol 48:126–135. doi:10.1016/j.ympev.2008.02.006
McAlpine DF, Fletcher TJ, Osepchook MA et al (1999) A range extension for Orconectes virilis (Decapoda, Cambaridae) and a third crayfish species for New Brunswick, Canada. Crustaceana 72:356–358
McAlpine DF, McAlpine AHE, Madden A (2007) Occurrence of the potentially invasive crayfish, Orconectes virilis (Decapoda, Cambaridae) in eastern New Brunswick, Canada. Crustaceana 80:509–511. doi:10.1163/156854007780440939
Page LM (1985) The crayfishes and shrimps (Decapoda) of Illinois. Ill Nat Hist Surv Bull 33:335–448
Pöckl M, Holdich DM, Pennerstorfer J (2006) Identifying native and alien crayfish species in Europe. European Project CRAYNET, Université de Poitiers, France
Posada D, Crandall KA (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817–818. doi:10.1093/bioinformatics/14.9.817
Pryor CW, Leone CA (1952) Serological comparisons of astacuran Crustacea. Biol Bull 103:433–445. doi:10.2307/1538425
Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. doi:10.1093/bioinformatics/btg180
Scholtz G, Braband A, Tolley L et al (2003) Parthenogenesis in an outsider crayfish. Nature 421:806. doi:10.1038/421806a
Skurdal J, Taugbøl T, Burba A et al (1999) Crayfish introductions in the Nordic and Baltic countries. In: Gherardi F, Holdich DM (eds) Crayfish in Europe as alien species. How to make the best of a bad situation? AA Balkema, Rotterdam, Brookfield, pp 193–219
Soes M (2007) Orconectes virilis: a North American crayfish conquering the Netherlands. Abstract book, 15th international conference on aquatic invasive species, Nijmegen, the Netherlands, p 85
Souty-Grosset C, Holdich DM, Noël PY et al. (2006) Atlas of crayfish in Europe. Muséum national d’Histoire naturelle, Patrimoines naturels, 64, Paris
Tamura K, Dudley J, Nei M et al (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599. doi:10.1093/molbev/msm092
Taylor CA, Hardman M (2002) Phylogenetics of the crayfish subgenus Crockerinus, genus Orconectes (Decapoda: Cambaridae), based on cytochrome oxidase I. J Crustacean Biol 22:874–881. doi:10.1651/0278-0372(2002)022[0874:POTCSC]2.0.CO;2
Taylor CA, Knouft JH (2006) Historical influences on genital morphology among sympatric species: gonopod evolution and reproductive isolation in the crayfish genus Orconectes (Cambaridae). Biol J Linn Soc Lond 89:1–12. doi:10.1111/j.1095-8312.2006.00637.x
Taylor CA, Schuster GA, Cooper JE et al (2007) A reassessment of the conservation status of crayfishes of the United States and Canada after 10+ years of increased awareness. Fisheries 32:372–389. doi:10.1577/1548-8446(2007)32[372:AROTCS]2.0.CO;2
Trontelj P, Machino Y, Sket B (2005) Phylogenetic and phylogeographic relationships in the crayfish genus Austropotamobius inferred from mitochondrial COI gene sequences. Mol Phylogenet Evol 34:212–226. doi:10.1016/j.ympev.2004.09.010
Acknowledgments
This study has been funded by the Grant Agency of the Academy of Sciences of the Czech Republic (project IAA601870701) and the Czech Ministry of Education (project MSM0021620828). Zdeněk Ďuriš provided the sample of O. virilis from Iowa, Bram Koese samples from the Netherlands, and Adam Ellis from the River Lee (UK). We thank Daniel Simberloff for corrections improving the previous version of the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Filipová, L., Holdich, D.M., Lesobre, J. et al. Cryptic diversity within the invasive virile crayfish Orconectes virilis (Hagen, 1870) species complex: new lineages recorded in both native and introduced ranges. Biol Invasions 12, 983–989 (2010). https://doi.org/10.1007/s10530-009-9526-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10530-009-9526-0