Abstract
Methanotrophs of the genus Methylocystis are frequently found in rice paddies. Although more than ten facultative methanotrophs have been reported since 2005, none of these strains was isolated from paddy soil. Here, a facultative methane-oxidizing bacterium, Methylocystis iwaonis SD4, was isolated and characterized from rhizosphere samples of rice plants in Nanjing, China. This strain grew well on methane or methanol but was able to grow slowly using acetate or ethanol. Moreover, strain SD4 showed sustained growth at low concentrations of methane (100 and 500 ppmv). M. iwaonis SD4 could utilize diverse nitrogen sources, including nitrate, urea, ammonium as well as dinitrogen. Strain SD4 possessed genes encoding both the particulate methane monooxygenase and the soluble methane monooxygenase. Simple and rapid genetic manipulation methods were established for this strain, enabling vector transformation and unmarked genetic manipulation. Fast growth rate and efficient genetic tools make M. iwaonis SD4 an ideal model to study facultative methanotrophs, and the ability to grow on low concentration of methane implies its potential in methane removal.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. 32270127). The authors are grateful to Chun Qin for his help in the operation of electron microscopy.
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This work was supported by the National Natural Science Foundation of China (No. 32270127, 32170126) and the Fundamental Research Funds for the Central Universities (KYCYXT2022003).
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YW: Strain isolation and characterization, Writing. YW: Genetic tools development, Writing. KZ: Electroporation method, Writing. HZ: Genome analysis, Writing. MC: Writing – review & editing. BW: Writing – review & editing. XY: Design of this work, Writing – review & editing.
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Wang, Y., Wang, Y., Zhou, K. et al. Isolation of a facultative methanotroph Methylocystis iwaonis SD4 from rice rhizosphere and establishment of rapid genetic tools for it. Biotechnol Lett (2024). https://doi.org/10.1007/s10529-024-03495-y
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DOI: https://doi.org/10.1007/s10529-024-03495-y