Fig. 5

Spot assay used in viability assay performed with Schizosaccharomyces pombe in the presence of bisphenol A and non-fermented or fermented oilseed cake extracts. Cells were grown at 30 °C for 6 h at 200 rpm. Positive control contained yeast cells grown in YES medium; Genotoxic control contained yeast cells in YES medium and 0.75 mM of bisphenol A; the Treatment contained yeast cells in YES medium, the genotoxic agent, and 0.75 g l−1 of extract. Each figure represents the test of a different extract, and each column represents serial dilutions from 10–1 until 10–4. The photographs shown are representative of three independent experiments