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Physicochemical and histological analysis of an experimental endodontic repair material containing 45S5 bioactive glass

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Abstract

This study aimed to evaluate the maximum compressive strength, the modulus of elasticity, pH variation, ionic release, radiopacity and biological response of an experimental endodontic repair cement based on 45S5 Bioglass®. An in vitro and in vivo study with an experimental endodontic repair cement containing 45S5 bioactive glass was conducted. There were three endodontic repair cement groups: 45S5 bioactive glass-based (BioG), zinc oxide-based (ZnO), and mineral trioxide aggregate (MTA). In vitro tests were used to evaluate their physicochemical properties: compressive strength, modulus of elasticity, radiopacity, pH variation, and the ionic release of Ca+ and PO4. An animal model was used to evaluate the bone tissue response to endodontic repair cement. Statistical analysis included the unpaired t-test, one-way ANOVA and Tukey’s test. BioG showed the lowest compressive strength and ZnO showed the highest radiopacity among the groups, respectively (p < 0.05). There were no significant differences in the modulus of elasticity among the groups. BioG and MTA maintained an alkaline pH during the 7 days of evaluation, both at pH 4 and in a pH 7 buffered solutions. PO4 was elevated in BioG, peaking at 7 days (p < 0.05). Histological analysis showed less intense inflammatory reactions and new bone formation in MTA. BioG showed inflammatory reactions that decreased over time. These findings suggest that the BioG experimental cement had good physicochemical characteristics and biocompatibility required for bioactive endodontic repair cement.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the Foundation for Research and Scientific and Technological Development of Maranhão (FAPEMA) [Grant number 01451/16], and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Brasil (CAPES) [Finance Code 001].

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Authors and Affiliations

  1. School of Dentistry, CEUMA University, Imperatriz, Brazil

    Michael Ranniery Garcia Ribeiro

  2. Dentistry Graduate Program, Federal University of Maranhão, Avenida dos Portugueses, 1966, Campus Universitário do Bacanga, São Luís, MA, 65085-580, Brazil

    Michael Ranniery Garcia Ribeiro, Vandilson Rodrigues, José Bauer & Soraia de Fátima Carvalho Souza

  3. School of Dentistry, Federal University of Maranhão, São Luís, Brazil

    Hudson Guterres Guilherme, Alina Neres Braga & Érika Martins Pereira

  4. Graduate Program in Microbial Biology, CEUMA University, São Luis, Brazil

    Afonso Gomes Abreu

Authors
  1. Michael Ranniery Garcia Ribeiro
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  2. Hudson Guterres Guilherme
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  3. Alina Neres Braga
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  4. Afonso Gomes Abreu
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  5. Érika Martins Pereira
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  6. Vandilson Rodrigues
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  7. José Bauer
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  8. Soraia de Fátima Carvalho Souza
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Contributions

MRGR: conceptualization, data curation, formal analysis, investigation, methodology, software, supervision, writing—review and editing. HGG: data curation, formal analysis, investigation. ANB: data curation, formal analysis, investigation. AGAJ: conceptualization, data curation, formal analysis, investigation, writing—original draft. ÉMP: formal analysis, investigation, methodology. VR: conceptualization, data curation, formal analysis, investigation, methodology, software, supervision, writing—review and editing. JB: formal analysis, investigation, methodology. SdFCS: conceptualization, data curation, formal analysis, funding acquisition, investigation, methodology, project administration, writing—review and editing.

Corresponding author

Correspondence to Vandilson Rodrigues.

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Conflict of interest

The authors declare that they do not have any conflict of interest.

Ethics approval

This study was approved by the Ethics Committee on Animal Use of CEUMA University (no. 06/2017).

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Ribeiro, M.R.G., Guilherme, H.G., Braga, A.N. et al. Physicochemical and histological analysis of an experimental endodontic repair material containing 45S5 bioactive glass. Biotechnol Lett 45, 799–809 (2023). https://doi.org/10.1007/s10529-023-03391-x

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  • DOI: https://doi.org/10.1007/s10529-023-03391-x

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