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Extraction of Eucommia ulmoides gum and microbial lipid from Eucommia ulmoides Oliver leaves by dilute acid hydrolysis

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Abstract

Objectives

Eucommia ulmoides gum (EUG) is an important natural biomass rubber material, which is usually extracted from Eucommia ulmoides Oliver (EUO). In the extraction process of EUG, pretreatment is the most important step which can efficiently damage EUG-containing cell wall and improve yield of EUG.

Results

The FT-IR, XRD, DSC and TG results showed that the thermal properties and structure of the EUG from the dilute acids hydrolysis residue are similar with that of the EUG directly extracted from EUO leaves (EUGD). EUO leaves hydrolysis with AA had the highest EUG yield (16.1%), which was higher than the EUGD yield (9.5%). In the case of the EUO leaves hydrolysis with 0.33 ~ 0.67 wt% of acetic acid (AA), the total sugar was stable in the range of 26.82–27.67 g/L. Furthermore, the EUO leaves acid hydrolysate (AA as reagent) was used as carbon sources for lipid-producing fermentation by Rhodosporidium toruloides. After 120 h of fermentation, the biomass, lipid content and lipid yield were 12.13 g/L, 30.16% and 3.64 g/L, respectively. The fermentation results indicated organic acids were no toxic for Rhodosporidium toruloides and the AA also could be used as carbon source for fermentation.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors acknowledge the financial support of the Henan province-CAS scientific and technical payoffs transformation project (2021114), Key Area R&D Project of Guangdong Province (2019B110209003), the Guangzhou Science and Technology Planning Project (202201010229) and the Project of National Natural Science Foundation of China (52006229, 51876207).

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Authors and Affiliations

  1. Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, 510640, People’s Republic of China

    Ruiling Gao, Hairong Zhang, Bo Li, Haijun Guo, Hailong Li, Lian Xiong & Xinde Chen

  2. Guangdong Key Laboratory of New and Renewable Energy Research and Development, Guangzhou, 510640, People’s Republic of China

    Ruiling Gao, Hairong Zhang, Bo Li, Haijun Guo, Hailong Li, Lian Xiong & Xinde Chen

  3. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People’s Republic of China

    Ruiling Gao, Hairong Zhang, Bo Li, Haijun Guo, Hailong Li, Lian Xiong & Xinde Chen

  4. R&D Center of Xuyi Attapulgite Applied Technology, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Xuyi, 211700, People’s Republic of China

    Ruiling Gao, Hairong Zhang, Bo Li, Haijun Guo, Hailong Li, Lian Xiong & Xinde Chen

  5. University of Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China

    Bo Li

Authors
  1. Ruiling Gao
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  2. Hairong Zhang
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  3. Bo Li
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Contributions

HZ: Investigation, Conceptualization, Methodology, Writing-review & editing. RG: Investigation, Methodology, Validation, Writing-review & editing. BL: Investigation and Formal analysis. HG: Data curation, Formal analysis. HL: Formal analysis and Validation. LX: Validation. XC: Project administration, Resources and Supervision.

Corresponding authors

Correspondence to Hairong Zhang or Xinde Chen.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Gao, R., Zhang, H., Li, B. et al. Extraction of Eucommia ulmoides gum and microbial lipid from Eucommia ulmoides Oliver leaves by dilute acid hydrolysis. Biotechnol Lett 45, 619–628 (2023). https://doi.org/10.1007/s10529-023-03377-9

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  • DOI: https://doi.org/10.1007/s10529-023-03377-9

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