Abstract
In this study, citric acid (CA) production by autochthonous Candida zeylanoides 7.12 was investigated and optimized. Response surface methodology (RSM) was used for the analysis of simultaneous effects of the chosen factors and 2 experiment designs were applied. In the first experimental design, the effects of initial pH value (5.5, 6.0 and 6.5), fermentation time (4, 5 and 6 days) and initial glucose concentration (125, 150 and 175 g/L) on CA production were investigated. Initial pH value was adjusted periodically with NaOH. Results of the statistical analysis showed that the model was found to be not applicable sufficiently to the chosen data. A second experimental design was employed at the same levels of glucose concentration and fermentation time by disabling the pH factor. pH level was kept at 6.5 with CaCO3. Results of the statistical analysis showed that the fit of the model was good and the lack of fit was not significant (P > 0.05). The highest CA concentration of 11.36 g/L was obtained after 6 days of fermentation with an initial glucose concentration of 125 g/L. The results indicated that initial glucose concentration and fermentation time were important parameters for CA production by C. zeylanoides 7.12 and this strain could be used for future studies.
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Abbreviations
- Adj MS:
-
Adjusted mean square
- Adj SS:
-
Adjusted sum of square
- CA:
-
Citric acid
- CA/ICA:
-
Citric acid/isocitric acid ratio
- DF:
-
Degrees of freedom
- GR :
-
Residual glucose concentration
- ICA:
-
Isocitric acid
- pH0 :
-
Initial pH value
- qCA :
-
Specific citric acid production rate
- SCA :
-
Selectivity of the bioprocess
- SE coefficient:
-
Standard error of the coefficient
- Seq SS:
-
Sequential sum of squares
- t:
-
Fermentation time
- X:
-
Biomass
- YCA/glucose :
-
Mass yield coefficient
- YCA/t :
-
Volumetric yield
- YCA/X :
-
Citric acid yield on biomass
- YX/glucose :
-
Biomass yield
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Acknowledgements
This project was supported by TUBITAK (Turkish Scientific and Technical Research Council), Project Number: 119O739. The authors would also like to thank Atatürk University Eastern Anatolia High Technology Application and Research Center (DAYTAM) Directorate.
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Funding was provided by Türkiye Bilimsel ve Teknolojik Araştirma Kurumu (Grant No.: 119O739).
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Sayın Börekçi, B., Kaya, M., Göksungur, Y. et al. Citric acid production by a novel autochthonous Candida zeylanoides isolate: optimization of process parameters. Biotechnol Lett 44, 803–812 (2022). https://doi.org/10.1007/s10529-022-03260-z
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DOI: https://doi.org/10.1007/s10529-022-03260-z