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Recent developments in miRNA based recombinant protein expression in CHO

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Abstract

It is widely accepted that the growing demand for recombinant therapeutic proteins has led to the expansion of the biopharmaceutical industry and the development of strategies to increase recombinant protein production in mammalian cell lines such as SP2/0 HEK and particularly Chinese hamster ovary cells. For a long time now, most investigations have been focused on increasing host cell productivity using genetic manipulating of cellular processes like cell cycle, apoptosis, cell growth, protein secretory and other pathways. In recent decades MicroRNAs beside different genetic engineering tools (e.g., TALEN, ZFN, and Crisper/Cas) have attracted further attention as a tool in the genetic engineering of host cells to increase protein expression levels. Their ability to simultaneously target multiple mRNAs involved in one or more cellular processes made them a favorable tool in this field. Accordingly, this study aimed to review the methods of selecting target miRNA for cell line engineering, miRNA gain- or loss-of-function strategies, examples of laboratory and pilot studies in this field and discussed advantages and disadvantages of this technology.

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Acknowledgements

We also thank Dr. Morteza Karimipour and Dr.Marjan Mohammadi (PhD) for their general support and editorial assistance.

Funding

The authors acknowledge the financial support received from the National Science Foundation (INSF) Development Program (INSF ID No.92006929), for their support and encouragement in carrying out this work.

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Authors and Affiliations

  1. Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

    Masoume Bazaz, Ahmad Adeli, Mohammad Azizi, Fereidoun Mahboudi & Noushin Davoudi

  2. Hematology and Cell Therapy Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Masoud Soleimani

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  1. Masoume Bazaz
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  2. Ahmad Adeli
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Bazaz, M., Adeli, A., Azizi, M. et al. Recent developments in miRNA based recombinant protein expression in CHO. Biotechnol Lett 44, 671–681 (2022). https://doi.org/10.1007/s10529-022-03250-1

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