Abstract
Objective
The degradation activity of two bacteriophages UPMK_1 and UPMK_2 against methicillin-resistant Staphylococcus aureus phages were examined using gel zymography.
Methods
The analysis was done using BLASTP to detect peptides catalytic domains. Many peptides that are related to several phage proteins were revealed.
Results
UPMK_1 and UPMK_2 custom sequence database were used for peptide identification. The biofilm-degrading proteins in the bacteriophage UPMK_2 revealed the same lytic activity towards polysaccharide intercellular adhesin-dependent and independent of Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers in comparison to UPMK_1, which had lytic activity restricted solely to its host.
Conclusion
Both bacteriophage enzymes were involved in MRSA biofilm degradation during phage infection and they have promising enzybiotics properties against MRSA biofilm formation.
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Acknowledgements
We are grateful to the Ministry of Higher Education, Malaysia for supporting this study by the Fundamental Research Grant Scheme (FRGS/1/2015/SGOS/UPM/01/2). Also to the Mustansiryah University for providing the scholarship of Khulood Hamid Dakheel PhD study.
Funding
Fundamental Research Grant Scheme (FRGS/1/2015/SGOS/UPM/01/2) awarded by the Ministry of Higher Education, Malaysia.
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Conceptualization and experimental design: JRAO, KY, KHD, RAR, VKN; performed the experiments: KHD; data interpretation and manuscript writing: KHD, JRAO; bioinformatic analysis: NR, TGH, MNMI. All authors approved the manuscript.
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Dakheel, K.H., Abdul Rahim, R., Al-Obaidi, J.R. et al. Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus. Biotechnol Lett 44, 513–522 (2022). https://doi.org/10.1007/s10529-022-03229-y
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DOI: https://doi.org/10.1007/s10529-022-03229-y