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Effects of labeling human mesenchymal stem cells with superparamagnetic zinc–nickel ferrite nanoparticles on cellular characteristics and adipogenesis/osteogenesis differentiation

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Abstract

Objective

An attractive cell source for stem cell-based therapy are WJ-MSCs. Hence, tracking WJ-MSCs using non-invasive imaging procedures (such as MRI) and contrast agents (Zn0.5Ni0.5Fe2O4, NFNPs) are required to evaluate cell distribution, migration, and differentiation.

Results

Results showed that the bare and dextrin-coated NFNPs were internalized inside the WJ-MSCs and had no effect on the cell viability, proliferation, apoptosis, karyotyping, and morphology of WJ-MSCs up to 125 µg/mL. Besides, treated WJ-MSCs were differentiated into osteo/adipocyte-like cells. The expression of RUNX 2, SPP 1 (P < 0.05), and OCN (P > 0.05) genes in the WJ-MSCs treated with dextrin-coated NFNPs was higher than the untreated WJ-MSCs; and the expression of CFD, LPL, and PPAR-γ genes was reduced in WJ-MSCs treated with both NFNPs in comparison with the untreated WJ-MSCs (P > 0.05).

Conclusion

Overall, results showed that dextrin-coated NFNPs had no adverse effect on the cellular characteristics, proliferation, and differentiation of WJ-MSCs, and suggesting their potential clinical efficacy.

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Data availability

All data is made available and presented in the manuscript.

Abbreviations

WJ-MSCs:

Wharton’s jelly mesenchymal stem cells

LPL:

Lipoprotein lipase

CFD:

Complement factor D,

PPARγ:

Peroxisome proliferator-activated receptor-gamma,

OCN:

Bone gamma-carboxyglutamate protein,

SPP:

Secreted phosphoprotein 1

RUNX 2:

Runt related transcription factor 2, and

NFNPs:

Zn0.5Ni0.5Fe2O4 nanoparticles

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Acknowledgement

The present article was supported by the Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran and Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Nanomedicine and Nanobiology Research Center, Shiraz University of Medical. The study team would like to gratefully acknowledge to staff of this center for their sincere cooperation.

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Authors and Affiliations

  1. Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran

    Solaleh Ghanbarei

  2. Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Solaleh Ghanbarei, Farzaneh Zarghampoor, Negar Azarpira & Mahdokht Hossein-Aghdaie

  3. Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

    Naghmeh Sattarahmady

  4. The Nanobiology and Nanomedicine Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Naghmeh Sattarahmady

  5. Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Khalili St, Mohamad Rasoolalah Research Tower, 7th floor, Shiraz, Iran

    Farzaneh Zarghampoor & Negar Azarpira

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  1. Solaleh Ghanbarei
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Correspondence to Farzaneh Zarghampoor or Negar Azarpira.

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This research was approved by the council and ethics committee of Shiraz University of medical Sciences-Iran with the ethical gridlines of the Helsinki Declaration of 1975 (the ethics code IR.SUMS.REC. 1397.752).

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Ghanbarei, S., Sattarahmady, N., Zarghampoor, F. et al. Effects of labeling human mesenchymal stem cells with superparamagnetic zinc–nickel ferrite nanoparticles on cellular characteristics and adipogenesis/osteogenesis differentiation. Biotechnol Lett 43, 1659–1673 (2021). https://doi.org/10.1007/s10529-021-03134-w

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