Abstract
Objective
Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolactone (PCL), an aliphatic polyester, as an effective solid organic substrate for the enrichment of marine Mn-oxidizing microorganisms.
Results
We successfully obtained marine microbial enrichment with the capacity for dissolved Mn removal and MnOx production using PCL as a solid organic substrate. The removal of dissolved Mn by the Mn-oxidizing enrichment culture followed first-order kinetics with a rate constant of 0.014 h−1. 16S rRNA gene amplicon sequencing analysis revealed that the Mn-oxidizing enrichment culture was highly dominated by operational taxonomic units related to the bacterial phyla Cyanobacteria, Planctomycetes, and Proteobacteria.
Conclusions
Our data demonstrate that PCL can serve as a potential substrate to enrich Mn-oxidizing microorganisms with the ability to produce MnOx under marine conditions.
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This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 20K15222.
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MA, TW, and TY contributed to the study conception and design. Material preparation, data collection, and analysis were performed by MA, YM, PTT, and YO. The first draft of the manuscript was written by MA, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Aoki, M., Miyashita, Y., Tran, P.T. et al. Enrichment of marine manganese-oxidizing microorganisms using polycaprolactone as a solid organic substrate. Biotechnol Lett 43, 813–823 (2021). https://doi.org/10.1007/s10529-021-03088-z
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DOI: https://doi.org/10.1007/s10529-021-03088-z