Abstract
Objectives
A redox potential-driven fermentation, maintaining dissolved oxygen at a prescribed level while simultaneously monitoring the changes of fermentation redox potential, was developed to guide the cultivation progress of recombinant protein expression.
Results
A recombinant E. coli harboring prolinase-expressing plasmid (pKK-PepR2) was cultivated using the developed process. Two distinct ORP valleys were noticeable based on recorded profile. The first ORP valley is equivalent to the timing for the addition of inducing agent, and the second ORP valley serves to guide the timing for cell harvesting. The final prolinase activity is 0.172 μmol/mg/min as compared to that of 0.154 μmol/mg/min where the optical density was employed to guide the timing of inducer addition and an empirically determined length of the cultivation.
Conclusion
The developed process can be further modified to become an automatic operation.
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Abbreviations
- DO:
-
Dissolved oxygen
- IPTG:
-
Isopropyl β-d-1-thiogalactopyranoside
- OD:
-
Optical density
- ORP:
-
Fermentation redox potential
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Funding
The authors acknowledge the financial support from the Natural Sciences and Engineering Research Council of Canada.
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Guo, J., Wu, Y., Tanaka, T. et al. Development of redox potential-driven fermentation process for recombinant protein expression. Biotechnol Lett 43, 99–103 (2021). https://doi.org/10.1007/s10529-020-03030-9
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DOI: https://doi.org/10.1007/s10529-020-03030-9