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Metabolomics on serum levels and liver of male Tupaia belangeri from 12 locations in China by GC–MS

Biotechnology Letters volume 42, pages 2561–2567 (2020)Cite this article

Abstract

Objectives

To investigate adaptive strategies of Tupaia belangeri to environmental factors in different populations, 12 locations were selected, including higher and lower altitude areas.

Results

Total of 96 and 90 metabolites were annotated in serum and liver respectively, which were mainly concentrated in primary metabolites. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) showed that the locations were divided into two groups in serum metabolites, but each group had a few samples overlap. The samples of each group overlap to some degree in the liver metabolites. The tricarboxylic acid (TCA) cycle occupies a central position in metabolism. The concentrations of TCA intermediates, lipid metabolites and amino acid metabolites were higher in higher altitude areas, and the concentrations of carbohydrate and glycolysis intermediates were higher in lower altitude areas.

Conclusions

Different areas adapted to the changes of environmental and altitude by regulating the concentration of metabolites in serum and liver, and revealed the adaptive mechanism of T. belangeri in different living environments.

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Acknowledgements

This work was supported by the National Science Foundation of China (31760118), and Young and Middle-aged Academic and Technical Leaders Reserve Talents Project of Yunnan Province (2019HB013). We wish to thank Pro. Burkart Engesser at Historisches Museum Basel, Switzerland for correcting the English usage in the draft. Thank you for the anonymous reviewers and the editor of the journal for their valuable comments.

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Affiliations

  1. Key Laboratory of Adaptive Evolution and Ecological Conservation on Plants and Animals in Southwest Mountain Ecosystem of Yunnan Higher Education Institutes, School of Life Sciences, Yunnan Normal University, Kunming, 650500, Yunnan, People’s Republic of China

    Dongmin Hou, Wenrong Gao, Wanlong Zhu & Zhengkun Wang

  2. Yunnan College of Business Management, Kunming, 650106, Yunnan, People’s Republic of China

    Ting Jia & Di Zhang

Authors
  1. Dongmin Hou
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  2. Ting Jia
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  3. Di Zhang
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  4. Wenrong Gao
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  5. Wanlong Zhu
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  6. Zhengkun Wang
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by DH, TJ, DZ, WG and WZ. The first draft of the manuscript was written by DH and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Wanlong Zhu.

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Conflict of interest

The authors declare no conflict of interest.

Ethical approval

All applicable the Animal Care and Use Committee of the School of Life Sciences, Yunnan Normal University for the care and use of animals were followed (No. 13-0901-011).

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Hou, D., Jia, T., Zhang, D. et al. Metabolomics on serum levels and liver of male Tupaia belangeri from 12 locations in China by GC–MS. Biotechnol Lett 42, 2561–2567 (2020). https://doi.org/10.1007/s10529-020-02988-w

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  • DOI: https://doi.org/10.1007/s10529-020-02988-w

Keywords

  • Tupaia belangeri
  • Metabolomics
  • Serum levels
  • Liver
  • Altitude
  • Temperature