Abstract
Objectives
To determine the secondary metabolite content, antioxidant and phenylalanine ammonia-lyase (PAL) activity as well as essential oil composition of in vitro cultures and field grown rhizomes of Zingiber montanum.
Results
Methyl jasmonate-treated cell cultures showed the highest total phenolic content and steroid content of 22.23 mg gallic acid equivalent/g dry weight (DW) and 41.67 mg/g DW, respectively. Callus cultures exhibited the highest tannin content (39.53 mg tannic acid equivalent/g DW) and strongest antioxidant activity (91.05% inhibition of 2,2-Diphenyl-1-picrylhydrazyl or DPPH). The highest saponin (81.76 mg/g DW) and alkaloid (113.97 mg/g DW) contents were obtained in in vitro microrhizomes induced on Murashige and Skoog (MS) medium supplemented with 6% sucrose and 5 mg/l 6-Benzylaminopurine (BAP), and MS medium supplemented with 7% sucrose, respectively. The essential oil content varied in cell cultures and microrhizomes and mainly consisted of fatty acid esters, which are precursors of many secondary metabolites. Trace amounts of terpinen-4-ol (0.21 and 0.27 mg/g) and zerumbone (0.0107 mg/g) were also detected in the in vitro microrhizomes.
Conclusion
The results obtained indicate the potential of in vitro cultures of Z. montanum for the production of secondary metabolites.
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Acknowledgements
The authors are grateful to Dr. Upendra Nongthomba (Department of molecular reproduction, development and genetics, IISC, Bangalore) and Mr. Muralidhar Nayak (Spectroscopy Analytical Test Facility, Society for innovation and development, IISC, Bangalore) for their kind assistance and technical support in GC/MS analysis. The authors would also like to thank Dr. Joel James (Structural Biology Lab, Center for Bio-Medical Research, VIT University) for his technical support in HPLC analysis.
Funding
This research was supported by Department of Biotechnology (DBT), New Delhi, under Grant No. BT/Bio-CARE/05/880/2010-11.
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Rajkumari, S., Sanatombi, K. Secondary metabolites content and essential oil composition of in vitro cultures of Zingiber montanum (Koenig) Link ex A. Dietr.. Biotechnol Lett 42, 1237–1245 (2020). https://doi.org/10.1007/s10529-020-02872-7
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DOI: https://doi.org/10.1007/s10529-020-02872-7