Abstract
Objective
Asthma is a chronic immune disease that has become a serious public health problem. The currently available medications are not ideal because of their limitations and side effects; hence, new target proteins and signaling cascades for precise and safe therapy treatment are needed. This work established an ovalbumin-induced asthma rat model and treated it with total flavonoid extract from the Xinjiang chamomile. The proteins that were differentially expressed in the chamomile extract-treated asthmatic rats and the asthma and healthy rat groups were identified using isobaric tagging followed by LC-MS/MS. Kyoto encyclopedia of genes and genomes pathway analysis of the differentially expressed proteins was performed.
Results
Pathways involved in purine metabolism, herpes simplex infection, and JNK phosphorylation and activation mediated by activated human TAK1 were enriched, indicating the intrinsic links between the mechanism of asthma development and treatment effects. Furthermore, we constructed a protein–protein interaction network and identified KIF3A as a potential target protein of chamomile extract that affected the Hedgehog signaling pathway.
Conclusions
This study may provide new insights into the pathogenesis of asthma and reveal several proteins and pathways that could be exploited to develop novel treatment approaches.
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Data availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
This work was supported by Xinjiang Uygur Autonomous Region Natural Science Fund (2018D01C284).
Supporting information
Fig. S1—Lung tissues proteins identified by LC-MS/MS. (A) is peptides length distribution and (B) is protein molecular weight and coverage distribution.
Fig S2—Quantitative analysis of protein expression between G and M group. (A) as well as M and N group (B) by Image J software. The expression was normalized to β-actin. The data were reported as mean ± SD, n = 3. **significant difference, P < 0.01; *significant difference, P < 0.05.
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QL and JZ made substantial contributions to conception and design, acquisition of data, analysis and interpretation of data; JL, XK and HZ performed the experiments; GZ and FZ have been involved in drafting the manuscript or revising it critically for important intellectual content; JN, XY and XX given final approval of the version to be published. HAA agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. (Laboratory Animal Ethics Committee of Xinjiang Medical University, NO: IACUC20161018-01).
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10529_2020_2825_MOESM1_ESM.tif
Electronic supplementary material 1 Fig S1. Lung tissues proteins identified by LC-MS/MS. (A) is peptides length distribution and (B) is protein molecular weight and coverage distribution. (TIF 1530 kb)
10529_2020_2825_MOESM2_ESM.tif
Electronic supplementary material 2 Fig. S2. Quantitative analysis of protein expression between G and M group. (A) as well as M and N group (B) by Image J software. The expression was normalized to β-actin. The data were reported as mean ± SD, n = 3. **significant difference, P < 0.01; *significant difference, P < 0.05. (TIF 79 kb)
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Li, Q., Zhao, S., Lu, J. et al. Quantitative proteomics analysis of the treatment of asthma rats with total flavonoid extract from chamomile. Biotechnol Lett 42, 905–916 (2020). https://doi.org/10.1007/s10529-020-02825-0
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DOI: https://doi.org/10.1007/s10529-020-02825-0