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Protective effect of Lactobacillus casei HY2782 against particulate matter toxicity in human intestinal CCD-18Co cells and Caenorhabditis elegans

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Abstract

Objectives

To investigate the preventive effect of Lactobacillus casei HY2782 on toxicity induced by particulate matter (PM, inhalable particles less than 10 μm in diameter) in human intestinal CCD-18Co cells and a model animal Caenorhabditis elegans.

Results

L. casei HY2782 treatment prevented PM-induced intestinal cell death via cellular reactive oxygen species production and membrane disruption attenuation. PM significantly decreased the total number of eggs laid and the body bending activity of C. elegans, demonstrating PM toxicity. L. casei HY2782 treatment restored the reproductive toxicity and decline in locomotion activity induced by PM in C. elegans. Overall, L. casei HY2782 attenuated PM toxicity in vitro in cultured intestinal cells and in vivo in the model nematode.

Conclusion

Our study provides a potential clue for developing L. casei HY2782 probiotics that attenuate PM-induced cellular and physiological toxicity; however, further in-depth preclinical trials using mammalian animal models and clinical trials are required.

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Acknowledgements

This research was funded by intramural grants from the Korea Institute of Science and Technology (KIST; 2Z05650, 2Z06220) and Korea Yakult. We thank Ms. Seemi Tasnim Alam and Ms. Tram Anh Ngoc Le (KIST, South Korea) for technical assistance related to C. elegans experiments

Supporting information

Supplementary Fig. 1 Effect of L. casei HY2782 feeding on the growth retardation induced by PM in C. elegans.

Author information

Authors and Affiliations

  1. Natural Product Informatics Research Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do, 25451, Republic of Korea

    Joo Yeon Kim, So Young Lee, Mi Ri Kim, Cheol-Ho Pan & Kyungsu Kang

  2. R&BD Center, Korea Yakult, Yongin, Gyeonggi-do, 17086, Republic of Korea

    Seung-Hee Jung, Il-Dong Choi, Jung-Lyoul Lee & Jae-Hun Sim

  3. Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology (UST), Gangneung, Gangwon-do, Republic of Korea

    Cheol-Ho Pan & Kyungsu Kang

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  1. Joo Yeon Kim
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  2. So Young Lee
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  3. Seung-Hee Jung
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  4. Mi Ri Kim
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  9. Kyungsu Kang
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Corresponding author

Correspondence to Kyungsu Kang.

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Conflict of interest

A portion of the technology described in this article (Korea patent, 10–2017-0177415) has been transferred from KIST to Korea Yakurt Co., Ltd. The funding sponsors had no roles in the design of the study; in the collection, analyses, or interpretation of data; or in the writing of the manuscript.

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This study does not contain any experiments involving human or mammalian subjects.

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Supplementary Fig. 1

Effect of L. casei HY2782 feeding on the growth retardation induced by PM in C. elegans. (PDF 89 kb)

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Kim, J.Y., Lee, S.Y., Jung, SH. et al. Protective effect of Lactobacillus casei HY2782 against particulate matter toxicity in human intestinal CCD-18Co cells and Caenorhabditis elegans. Biotechnol Lett 42, 519–528 (2020). https://doi.org/10.1007/s10529-020-02814-3

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  • DOI: https://doi.org/10.1007/s10529-020-02814-3

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