Abstract
Objectives
Although abnormal expression of early growth response-1 (Egr1) has been revealed in various human solid tumors, the functions and potential mechanisms of Egr1 in the progression of salivary gland pleomorphic adenoma (SGPA) are not entirely understood.
Results
An elevated expression of Egr1 was observed both in the human salivary gland pleomorphic adenoma tissues and tumor-initiating cell (TIC) cells, when compared with control group. By loss-of-function assay, the proliferation and invasion capacities of TICs were inhibited, while the cell apoptosis was promoted, which were further evidenced by the protein expression analysis of several key apoptosis-related regulators. Furthermore, TICs with Mithramycin A (an Egr1 inhibitor) treatment achieved the same effects of endogenous Egr1 knockdown.
Conclusions
All these data collectively suggest that Egr1 act as an oncogenic factor in salivary gland pleomorphic adenoma, which may be a potential target for the treatment of SGPA.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (81302359), Fundamental research program funding of Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine (JYZZ086B) and the 6th session of hospital outstanding Youth Funding of Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine.
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Supplementary Table 1—The sequences used in this article.
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Shen, S., Zhu, Y., Lu, H. et al. Transcription factor early growth response-1 plays an oncogenic role in salivary gland pleomorphic adenoma. Biotechnol Lett 42, 197–207 (2020). https://doi.org/10.1007/s10529-019-02776-1
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DOI: https://doi.org/10.1007/s10529-019-02776-1