Autochthonous lactic acid bacteria—presentation of potential probiotics application
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The objective of this study was to evaluate the probiotic potential as well as the ability of adhesion and aggregation of natural and autochthonous lactic acid bacteria, isolated from traditionally made cheese.
Lactic acid bacteria from natural food sources can be promising probiotic candidates and they can be used in natural food preservation or like starter cultures. Tested autochthonous isolates showed tolerance to the simulated gastrointestinal condition as well as the sensitivity to clinically relevant antibiotics, especially to ampicillin (MIC at 0.195 μg mL−1 for lactobacilli and from 0.195 to 3.125 μg mL−1 for lactococci). Among isolates, the highest percentage of adhesion was detected with chloroform, while the adhesion ability of selected isolates to pig intestinal epithelium was in the correlation with the results of adhesion ability with solvents. The auto-aggregation ability of isolates was demonstrated, while co-aggregation with Escherichia coli was strain specific.
The results indicated the potential probiotic properties of the isolates and give evidence for further investigation and potential application in the dairy industry.
KeywordsAntibiotic Adhesion Aggregation ability Lactic acid bacteria Probiotics Pig intestinal epithelium
This investigation was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 41010).
MŽG conceived and designed the experiments; DDN designed and photographed samples by florescent microscope and interpreted the results of adhesion; MŽG and KGM processed the results; KGM and LRČ take charge of the preparation of the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflicts of interest
The authors declare that they have no conflicts of interest with the current work or its publication.
Research involving human and animal participants
This article does not contain any studies with human participants or animals performed by any of the authors.
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