Abstract
Objectives
To enhance the productivity of foreign protein in culture cells using baculovirus expression system.
Results
A low concentration of MβCD, with the optimal application concentration of 0.25 mM and the appropriate preincubation time range from 10 to 120 min, can efficiently enhance expression levels in both the AcMNPV and BmNPV expression systems.
Conclusions
Preincubation with a low concentration MβCD enhance baculovirus infection and foreign protein expression productivity.
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Acknowledgements
This study was funded by the National Science Foundation of China (Grant No. 31670152); and the Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry (Grant No. 2015).
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Huang, J., Liu, N., Shen, X. et al. Preincubation with a low concentration of methyl-β-cyclodextrin enhances baculovirus expression system productivity. Biotechnol Lett 41, 921–928 (2019). https://doi.org/10.1007/s10529-019-02708-z
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DOI: https://doi.org/10.1007/s10529-019-02708-z