Abstract
Objective
A correlation among different volumetric oxygen transfer coefficients (kLa) and the oxireduction potential (ORP) in batch fermentations using Scheffersomyces stipitis was evaluated. Experiments were performed using a mixture of xylose and glucose as the substrates.
Results
Microaerophilic condition (kLa = 4.9 h−1) have shown to be suitable when compared to complete anaerobiosis (kLa = 0), providing an ethanol yield and a productivity after 48 h of 64.3% and 0.45 g ethanol L−1 h−1, respectively; the maximum ethanol titer obtained was 21.50 g ethanol L−1. Values of ORP varying from − 270 to − 330 mV resulted in high ethanol production from xylose by S. stipitis.
Conclusions
Different ORP values were found in anaerobiosis and kLa 4.9 h−1, suggesting that for ethanol production by S. stipitis, values from − 270 to − 330 mV are favorable under the studied circumstances. In this ORP range, the greatest rates of xylose consumption and ethanol production were registered. ORP monitoring was demonstrated to be a suitable option for online control throughout the fermentation processes, which may provide a more efficient bioprocess operation with a very low O2 concentration.
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Acknowledgements
The authors acknowledge the Brazilian Bioethanol Science and Technology Laboratory - CTBE/CNPEM. This study was funded by FAPESP (Grant Numbers 2016/06142-0 and 2017/049970).
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Bonan, C.I.D.G., Biazi, L.E., Santos, S.C. et al. Online monitoring of the redox potential in microaerobic and anaerobic Scheffersomyces stipitis fermentations. Biotechnol Lett 41, 753–761 (2019). https://doi.org/10.1007/s10529-019-02674-6
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DOI: https://doi.org/10.1007/s10529-019-02674-6