Abstract
Objectives
To evaluate the effects of the surface modification of 316L stainless steel (SS) by low-temperature plasma nitriding on endothelial cells for stent applications.
Results
X-ray diffraction (XRD) confirmed the incorporation of nitrogen into the treated steel. The surface treatment significantly increased SS roughness and hydrophilic characteristics. After 4 h the cells adhered to the nitride surfaces and formed clusters. During the 24 h incubation period, cell viability on the nitrided surface was higher compared to the polished surface. Nitriding reduced late apoptosis of rabbit aorta endothelial cell (RAEC) on the SS surface.
Conclusion
Low temperature plasma nitriding improved the biocompatible of stainless steel for use in stents.
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Acknowledgements
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. The authors wish to acknowledge the professional efforts of team of the Laboratory of Structural Characterization of Materials at UFRN and Dr. Helena B. Nader of UNIFESP, São Paulo, Brazil for contributing with the endothelial rabbit aorta cell line.
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Braz, J.K.F.S., Martins, G.M., Sabino, V. et al. Plasma nitriding under low temperature improves the endothelial cell biocompatibility of 316L stainless steel. Biotechnol Lett 41, 503–510 (2019). https://doi.org/10.1007/s10529-019-02657-7
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DOI: https://doi.org/10.1007/s10529-019-02657-7