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Mining of Egypt’s Red Sea invertebrates for potential bioactive producers

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Abstract

Objective

The objective of this work was to isolate bacteria from Red Sea invertebrates, determine their antimicrobial activity, and screen for the biosynthetic gene clusters [polyketides (PKs) and nonribosomal peptides (NRPs)] which could be involved in the production of bioactive secondary metabolites.

Result

Eleven different samples of marine invertebrates’ were collected from Egypt’s Red Sea (El-Tor-Sharm El-Sheikh and Hurghada) by scuba diving, and a total 80 isolates of the associated microorganisms were obtained from the cultivation on six different cultural medium. Seven isolates of them showed an antimicrobial activity against five pathogenic reference strains, while the most active antimicrobial agent was isolate number HFF-8 which was 99% identical to Bacillus amyloliquefaciens. HFF-8’s extract showed positive results against Gram negative bacteria, Gram positive bacteria and yeast. Moreover, the isolates gave positive bands when screened for the presence of PK synthase (PKS) I and II and NRP synthetase (NRPS) I and II biosynthetic genes, those biosynthetic fragments when cloned and sequenced were primitively predicted as biosynthetic fragments for kirromycin and leinamycin production by NaPDoS program with 56 and 55%, respectively.

Conclusion

The Red Sea can provide a sustainable solution to combat bacterial resistance. The contribution of this work is that B. amyloliquefaciens was isolated from Heteroxenia fuscescens, Red Sea, Egypt. Moreover, the bacterial extract showed a broad spectrum with a potent antimicrobial activity.

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Acknowledgements

We would like to thank Dr. Hossamuldin Elfeqy for his help in collecting marine invertebrates samples from Eltor- Sharm El sheikh.

Supporting information

Supplementary Table 1—Identification score criteria by Bruker Daltonics.

Supplementary Table 2—The primers used in screening or biosynthetic genes PKS I, II and NRPS I, II genes.

Supplementary Table 3—The amplification protocol of the primers used in screening for biosynthetic PKS I, II and NRPS I, II genes.

Supplementary Table 4—Classification of the isolates according to the type of the invertebrate and the type of the medium.

Supplementary Table 5—Identification of characteristic isolates using MALDI-TOF MS.

Supplementary Table 6—Screening of the isolates for biosynthetic gene fragments PKS I&II and NRPS I&II.

Supplementary Figure 1—Screening of the isolates for biosynthetic gene fragments NRPS I (primer 1) and NRPS II (primer 2).

Supplementary Figure 2—Screening of the isolates for biosynthetic gene fragments PKS I (primer 3) and PKS I (primer 4).

Supplementary Figure 3—Screening of the isolates for biosynthetic gene fragments PKS II (primer 5).

Author information

Authors and Affiliations

  1. Microbiology and Public Health Department, Faculty of Pharmacy, Heliopolis University for Sustainable Development, Cairo, Egypt

    Hala S. Helal

  2. Microbiology and Immunology Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt

    Amro Hanora

  3. Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Kasr Al-Aini, Cairo, 11562, Egypt

    Rania Abdelmonem Khattab & Hamdallah Zedan

  4. National Organization for Drug Control and Research, Giza, Egypt

    Hala S. Helal & Hayam Hamouda

Authors
  1. Hala S. Helal
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  2. Amro Hanora
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  3. Rania Abdelmonem Khattab
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  4. Hayam Hamouda
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  5. Hamdallah Zedan
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Corresponding author

Correspondence to Amro Hanora.

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Conflict of interest

None.

Additional information

Accession numbers: for 16S sequences: MG757672.1–MG757678.1; for PKS and NRPS sequences: MG975081–MG975083.

Electronic supplementary material

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Supplementary material 1 (DOCX 674 kb)

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Helal, H.S., Hanora, A., Khattab, R.A. et al. Mining of Egypt’s Red Sea invertebrates for potential bioactive producers. Biotechnol Lett 40, 1519–1530 (2018). https://doi.org/10.1007/s10529-018-2600-x

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  • DOI: https://doi.org/10.1007/s10529-018-2600-x

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