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Lactobacillus spp. impair the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells

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Abstract

Objectives

The objective of this study was to evaluate the ability of Lactobacillus curvatus CRL705, CRL1532, and CRL1533 and Lactobacillus sakei CRL1613 to survive under simulated gastrointestinal conditions. Moreover, a microencapsulation approach was proposed to improve gastrointestinal survival. Finally, experiments were performed to demonstrate that Lactobacillus spp. can modulate the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells.

Results

Lactobacillus strains were encapsulated in alginate beads to enhance the survival of bacteria under in vitro gastrointestinal conditions. All strains hydrolyzed bile salts using chenodeoxycholic acid as a substrate and adhered to Caco-2 cells. Cell-free supernatants (CFSs) showed antimicrobial activity against L. monocytogenes as demonstrated by agar diffusion assays. The average percentages of L. monocytogenes adhesion decreased from 67.74 to 41.75 and 38.7% in the presence of 50 and 90% (v/v), respectively, for all CFSs tested. The highest concentrations of CFSs completely inhibited the L. monocytogenes invasion of Caco-2 cells.

Conclusions

The studied Lactobacillus strains have protective effects against the adhesion and invasion of L. monocytogenes FBUNT. Alginate encapsulation of these bacteria improved gastrointestinal tolerance such that they could be further studied as potential probiotics against intestinal pathogenic bacteria.

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Acknowledgements

We are grateful to The Bilateral Cooperation Program CONICET-FAPESP (2013/50498-5) for financial support of this study. We also thank Lucas Oliveira Sousa and Vanessa Maciel de Souza from FCFRP-USP for their excellent technical assistance with the cell line assays.

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Authors and Affiliations

  1. Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, 4000, Tucumán, Argentina

    P. Castellano & M. Pérez Ibarreche

  2. Faculdade de Ciências Farmacêuticas de Ribeirão Preto – USP, Av. do Café s/n, Monte Alegre, Ribeirão Preto, SP, 14040-903, Brazil

    L. Longo Borges, F. C. Niño Arias & E. C. Pereira De Martinis

  3. Instituto de Biotecnología farmacéutica y alimentaria (INBIOFAL-CONICET), Av Kichner, 4000, Tucumán, Argentina

    G. R. Ross

Authors
  1. P. Castellano
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  2. M. Pérez Ibarreche
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  3. L. Longo Borges
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  4. F. C. Niño Arias
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  5. G. R. Ross
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  6. E. C. Pereira De Martinis
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Corresponding author

Correspondence to P. Castellano.

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Castellano, P., Pérez Ibarreche, M., Longo Borges, L. et al. Lactobacillus spp. impair the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells. Biotechnol Lett 40, 1237–1244 (2018). https://doi.org/10.1007/s10529-018-2572-x

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  • DOI: https://doi.org/10.1007/s10529-018-2572-x

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