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Stimulation of mouse vibrissal follicle growth by recombinant human fibroblast growth factor 20

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Abstract

Objectives

To explore potential effects of recombinant human fibroblast growth factor 20 (rhFGF20) in the growth of cultured mouse vibrissal follicles.

Results

The growth of cultured mouse vibrissal follicles was significantly induced by rhFGF20 in a dose dependent pattern in the in vitro vibrissal follicle organ culture model. However, too high concentration of rhFGF20 could inhibit the growth of vibrissal follicles. We further demonstrated that rhFGF20 stimulated the proliferation of hair matrix cells and activated Wnt/β-catenin signaling pathway.

Conclusions

The rhFGF20 might be a potential therapeutic agent to treat hair loss disorders.

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References

  • Beenken A, Mohammadi M (2009) The FGF family: biology, pathophysiology and therapy. Nat Rev Drug Discov 8:235–253

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Gay D, Kwon O, Zhang Z, Spata M, Plikus MV, Holler PD, Ito M, Yang Z, Treffeisen E, Kim CD, Nace A, Zhang X, Baratono S, Wang F, Ornitz DM, Millar SE, Cotsarelis G (2013) Fgf9 from dermal gammadelta T cells induces hair follicle neogenesis after wounding. Nat Med 19:916–923

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Guan L, Zhu S, Han Y, Yang C, Liu Y, Qiao L, Li X, Li H, Lin J (2017) Knockout of CTNNB1 by CRISPR-Cas9 technology inhibits cell proliferation through the Wnt/beta-catenin signaling pathway. Biotechnol Lett. https://doi.org/10.1007/s10529-017-2491-2

    Article  PubMed  Google Scholar 

  • Huh SH, Narhi K, Lindfors PH, Haara O, Yang L, Ornitz DM, Mikkola ML (2013) Fgf20 governs formation of primary and secondary dermal condensations in developing hair follicles. Genes Dev 27:450–458

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Langan EA, Philpott MP, Kloepper JE, Paus R (2015) Human hair follicle organ culture: theory, application and perspectives. Exp Dermatol 24:903–911

    Article  PubMed  Google Scholar 

  • Lim X, Nusse R (2013) Wnt signaling in skin development, homeostasis, and disease. Cold Spring Harb Perspect Biol. https://doi.org/10.1101/cshperspect.a008029

    Article  PubMed  PubMed Central  Google Scholar 

  • Merrill BJ, Gat U, DasGupta R, Fuchs E (2001) Tcf3 and Lef1 regulate lineage differentiation of multipotent stem cells in skin. Genes Dev 15:1688–1705

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Muller-Rover S, Handjiski B, van der Veen C, Eichmuller S, Foitzik K, McKay IA, Stenn KS, Paus R (2001) A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J Investig Dermatol 117:3–15

    Article  PubMed  CAS  Google Scholar 

  • Ornitz DM, Itoh N (2015) The Fibroblast Growth Factor signaling pathway. Wiley Interdiscip Rev Dev Biol 4:215–266

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Paus R, Cotsarelis G (1999) The biology of hair follicles. N Engl J Med 341:491–497

    Article  PubMed  CAS  Google Scholar 

  • Philpott M, Green MR, Kealey T (1989) Studies on the biochemistry and morphology of freshly isolated and maintained rat hair follicles. J Cell Sci 93(Pt 3):409–418

    PubMed  CAS  Google Scholar 

  • Philpott MP, Westgate GE, Kealey T (1991) An in vitro model for the study of human hair growth. Ann N Y Acad Sci 642:148–164; discussion 164–146

  • Tian H, Zhao Y, Chen N, Wu M, Gong W, Zheng J, Fernig DG, Jungbauer A, Wang D, Li X, Jiang C (2016) High production in E. coli of biologically active recombinant human fibroblast growth factor 20 and its neuroprotective effects. Appl Microbiol Biotechnol 100:3023–3034

    Article  PubMed  CAS  Google Scholar 

  • Valenta T, Hausmann G, Basler K (2012) The many faces and functions of beta-catenin. EMBO J 31:2714–2736

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Varothai S, Bergfeld WF (2014) Androgenetic alopecia: an evidence-based treatment update. Am J Clin Dermatol 15:217–230

    Article  PubMed  Google Scholar 

  • Wang TL, Zhou C, Shen YW, Wang XY, Ding XL, Tian S, Liu Y, Peng GH, Xue SQ, Zhou JE, Wang RL, Meng XM, Pei GD, Bai YH, Liu Q, Li H, Zhang JZ (2010) Prevalence of androgenetic alopecia in China: a community-based study in six cities. Br J Dermatol 162:843–847

    Article  PubMed  CAS  Google Scholar 

  • Whitehead GG, Makino S, Lien CL, Keating MT (2005) fgf20 is essential for initiating zebrafish fin regeneration. Science 310:1957–1960

    Article  PubMed  CAS  Google Scholar 

  • Xing Y, Xu W, Yang K, Lian X, Yang T (2011) Immunolocalization of Wnt5a during the hair cycle and its role in hair shaft growth in mice. Acta Histochem 113:608–612

    Article  PubMed  CAS  Google Scholar 

  • Xu XG, Chen HD (2017) Prostanoids and hair follicles: implications for therapy of hair disorders. Acta Dermatovenereol. https://doi.org/10.2340/00015555-2843

    Article  Google Scholar 

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Acknowledgements

This work was supported by National Natural Science Funding of China (81602740).

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Authors and Affiliations

  1. Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang, China

    Xue-Gang Xu, Lin Gong, Tian-Ling Jiang, Yuan-Hong Li, Xing-Hua Gao & Hong-Duo Chen

  2. School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, 325035, China

    Haishan Tian

Authors
  1. Xue-Gang Xu
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  2. Lin Gong
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  3. Tian-Ling Jiang
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  4. Yuan-Hong Li
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  5. Xing-Hua Gao
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  6. Haishan Tian
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  7. Hong-Duo Chen
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Corresponding authors

Correspondence to Haishan Tian or Hong-Duo Chen.

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Xu, XG., Gong, L., Jiang, TL. et al. Stimulation of mouse vibrissal follicle growth by recombinant human fibroblast growth factor 20. Biotechnol Lett 40, 1009–1014 (2018). https://doi.org/10.1007/s10529-018-2554-z

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  • DOI: https://doi.org/10.1007/s10529-018-2554-z

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