Biotechnology Letters

, Volume 40, Issue 6, pp 999–1007 | Cite as

Conditioned medium from umbilical cord mesenchymal stem cells improves nasal mucosa damage by radiation

  • Hong-Gang Duan
  • Fang Ji
  • Chun-Quan Zheng
  • Jing Li
  • Jing Wang
Original Research Paper



To explore therapeutic effects of conditioned medium from human umbilical cord mesenchymal stem cells (hUC-MSCs) on nasal mucosa radiation damage both in vivo and in vitro.


The mucus cilia clearance time (7 and 30 days), degree of mucosal edema (7, 30, 90 and 180 days), cilia coverage (180 days) of concentrated conditioned medium group improved compared with radiotherapy control group. The proliferation and migration abilities of irradiated and non-irradiated nasal epithelial cells significantly increased after culture in bronchial epithelial cell growth medium (BEGM) containing 10% conditioned medium of hUC-MSCs compared to cells cultured in BEGM alone.


Soluble factors secreted by hUC-MSCs may promote nasal epithelial cell proliferation and migration. Intranasal administration of hUC-MSC conditioned medium effectively repairs nasal mucosa radiation damage.


Cytokines Conditioned medium Mesenchymal stem cells Nasal mucosa Radiation damage 



This study was supported by Medicine and Health General Research Program of Zhejiang Province, No. 2016155085.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.


  1. Ando Y, Matsubara K, Ishikawa J, Fujio M, Shohara R, Hibi H, Ueda M, Yamamoto A (2014) Stem cell-conditioned medium accelerates distraction osteogenesis through multiple regenerative mechanisms. Bone 61:82–90. CrossRefPubMedGoogle Scholar
  2. Chen L, Tredget EE, Wu PY, Wu Y (2008) Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound healing. PLoS ONE 3:e1886. CrossRefPubMedPubMedCentralGoogle Scholar
  3. Dominici MLBK, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini FC, Krause DS et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8:315–317CrossRefPubMedGoogle Scholar
  4. Duan HG, Ji F, Zheng CQ, Wang CH, Li J (2014) Human umbilical cord mesenchymal stem cells alleviate nasal mucosa radiation damage in a guinea pig model. J Cell Biochem. Google Scholar
  5. Fan CG, Zhang QJ, Zhou JR (2011) Therapeutic potentials of mesenchymal stem cells derived from human umbilical cord. Stem Cell Rev 7:195–207. CrossRefPubMedGoogle Scholar
  6. Fong CY, Tam K, Cheyyatraivendran S, Gan SU, Gauthaman K, Armugam A, Jeyaseelan K, Choolani M, Biswas A, Bongso A (2014) Human Wharton’s jelly stem cells and its conditioned medium enhance healing of excisional and diabetic wounds. J Cell Biochem 115:290–302. CrossRefPubMedGoogle Scholar
  7. Ji F, Duan HG, Zheng CQ, Li J (2015) Comparison of chloromethyl-dialkylcarbocyanine and green fluorescent protein for labeling human umbilical mesenchymal stem cells. Biotechnol Lett 37:437–447. CrossRefPubMedGoogle Scholar
  8. Liang CC, Park AY, Guan JL (2007) In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2:329–333. CrossRefPubMedGoogle Scholar
  9. Lin CY, Chang FH, Chen CY, Huang CY, Hu FC, Huang WK, Ju SS, Chen MH (2011) Cell therapy for salivary gland regeneration. J Dent Res 90:341–346. CrossRefPubMedGoogle Scholar
  10. Liu J, Han Z, Han Z, He Z (2016) Mesenchymal stem cell-conditioned media suppresses inflammation-associated overproliferation of pulmonary artery smooth muscle cells in a rat model of pulmonary hypertension. Exp Ther Med 11:467–475. CrossRefPubMedGoogle Scholar
  11. Phulpin B, Dolivet G, Marie PY, Poussier S, Huger S, Bravetti P, Graff P, Merlin JL, Tran N (2011) Feasibility of treating irradiated bone with intramedullary delivered autologous mesenchymal stem cells. J Biomed Biotechnol 2011:560257. CrossRefPubMedPubMedCentralGoogle Scholar
  12. Shao D, Massoud E, Clarke D, Cowley E, Renton K, Agu RU (2013) Optimization of human nasal epithelium primary culture conditions for optimal proton oligopeptide and organic cation transporters expression in vitro. Int J Pharm 441:334–342. CrossRefPubMedGoogle Scholar
  13. Singer NG, Caplan AI (2011) Mesenchymal stem cells: mechanisms of inflammation. Annu Rev Pathol 6:457–478. CrossRefPubMedGoogle Scholar
  14. Song SW, Kim KE, Choi JW, Lee CY, Lee J, Seo HH, Lim KH, Lim S, Lee S, Kim SW, Hwang KC (2016) Proteomic analysis and identification of paracrine factors in mesenchymal stem cell-conditioned media under hypoxia. Cell Physiol Biochem 40:400–410. CrossRefPubMedGoogle Scholar
  15. Sumitsawan Y, Chaiyasate S, Chitapanarux I, Anansuthiwara M, Roongrotwattanasiri K, Vaseenon V, Tooncam H (2009) Late complications of radiotherapy for nasopharyngeal carcinoma. Auris Nasus Larynx 36:205–209. CrossRefPubMedGoogle Scholar
  16. Zhang J, Lv S, Liu X, Song B, Shi L (2018) Umbilical cord mesenchymal stem cell treatment for Crohn’s disease: a randomized controlled clinical trial. Gut Liver 12:73–78. CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Otolaryngology, Second Affiliated Hospital, College of MedicineZhejiang UniversityHangzhouChina
  2. 2.Department of NeurologyThe First Affiliated Hospital, Zhejiang University School of MedicineHangzhouChina
  3. 3.Department of Otolaryngology, Affiliated Eye and Ear, Nose and Throat Hospital, Shanghai Medical CollegeFudan UniversityShanghaiChina
  4. 4.Department of OtolaryngologyHangzhou First People’s HospitalHangzhouChina

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